Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru
ABSTRACT Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its...
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| Autores principales: | , |
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| Lenguaje: | English |
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Universidad de Tarapacá. Facultad de Ciencias Agronómicas
2018
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| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000200203 |
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| Sumario: | ABSTRACT Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its massive propagation in vitro. For the establishment stage, ½ MS was determined to be the best culture medium. In the proliferation stage, the growth regulator balance that showed the highest survival rate was MS + IBA 1 ppm + BA 0.5 ppm. Different combinations of NAA-AIB were tested for rooting; MS + NAA 0.005 ppm + IBA 0.005 ppm was determined as the best medium. In the last stage, 65% of in vitro plants were acclimatized using vermiculite as substrate and irrigating with 50% Hoagland mineral solution. |
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