Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru
ABSTRACT Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its...
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Universidad de Tarapacá. Facultad de Ciencias Agronómicas
2018
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oai:scielo:S0718-342920180002002032019-04-04Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of PeruMedina Medina,FiorellaLazo Rodríguez,Herbert Cantua volcánica micropropagation shoot proliferation ABSTRACT Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its massive propagation in vitro. For the establishment stage, ½ MS was determined to be the best culture medium. In the proliferation stage, the growth regulator balance that showed the highest survival rate was MS + IBA 1 ppm + BA 0.5 ppm. Different combinations of NAA-AIB were tested for rooting; MS + NAA 0.005 ppm + IBA 0.005 ppm was determined as the best medium. In the last stage, 65% of in vitro plants were acclimatized using vermiculite as substrate and irrigating with 50% Hoagland mineral solution.info:eu-repo/semantics/openAccessUniversidad de Tarapacá. Facultad de Ciencias AgronómicasIdesia (Arica) v.36 n.2 20182018-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000200203en10.4067/S0718-34292018005000501 |
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Scielo Chile |
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Scielo Chile |
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English |
topic |
Cantua volcánica micropropagation shoot proliferation |
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Cantua volcánica micropropagation shoot proliferation Medina Medina,Fiorella Lazo Rodríguez,Herbert Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru |
description |
ABSTRACT Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its massive propagation in vitro. For the establishment stage, ½ MS was determined to be the best culture medium. In the proliferation stage, the growth regulator balance that showed the highest survival rate was MS + IBA 1 ppm + BA 0.5 ppm. Different combinations of NAA-AIB were tested for rooting; MS + NAA 0.005 ppm + IBA 0.005 ppm was determined as the best medium. In the last stage, 65% of in vitro plants were acclimatized using vermiculite as substrate and irrigating with 50% Hoagland mineral solution. |
author |
Medina Medina,Fiorella Lazo Rodríguez,Herbert |
author_facet |
Medina Medina,Fiorella Lazo Rodríguez,Herbert |
author_sort |
Medina Medina,Fiorella |
title |
Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru |
title_short |
Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru |
title_full |
Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru |
title_fullStr |
Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru |
title_full_unstemmed |
Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru |
title_sort |
establishment of an in vitro micropropagation protocol for cantua volcánica (polemoniaceae): a potential ornamental plant of peru |
publisher |
Universidad de Tarapacá. Facultad de Ciencias Agronómicas |
publishDate |
2018 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000200203 |
work_keys_str_mv |
AT medinamedinafiorella establishmentofaninvitromicropropagationprotocolforcantuavolcanicapolemoniaceaeapotentialornamentalplantofperu AT lazorodriguezherbert establishmentofaninvitromicropropagationprotocolforcantuavolcanicapolemoniaceaeapotentialornamentalplantofperu |
_version_ |
1714203759640313856 |