Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru

ABSTRACT Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its...

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Autores principales: Medina Medina,Fiorella, Lazo Rodríguez,Herbert
Lenguaje:English
Publicado: Universidad de Tarapacá. Facultad de Ciencias Agronómicas 2018
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000200203
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spelling oai:scielo:S0718-342920180002002032019-04-04Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of PeruMedina Medina,FiorellaLazo Rodríguez,Herbert Cantua volcánica micropropagation shoot proliferation ABSTRACT Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its massive propagation in vitro. For the establishment stage, ½ MS was determined to be the best culture medium. In the proliferation stage, the growth regulator balance that showed the highest survival rate was MS + IBA 1 ppm + BA 0.5 ppm. Different combinations of NAA-AIB were tested for rooting; MS + NAA 0.005 ppm + IBA 0.005 ppm was determined as the best medium. In the last stage, 65% of in vitro plants were acclimatized using vermiculite as substrate and irrigating with 50% Hoagland mineral solution.info:eu-repo/semantics/openAccessUniversidad de Tarapacá. Facultad de Ciencias AgronómicasIdesia (Arica) v.36 n.2 20182018-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000200203en10.4067/S0718-34292018005000501
institution Scielo Chile
collection Scielo Chile
language English
topic Cantua volcánica
micropropagation
shoot proliferation
spellingShingle Cantua volcánica
micropropagation
shoot proliferation
Medina Medina,Fiorella
Lazo Rodríguez,Herbert
Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru
description ABSTRACT Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its massive propagation in vitro. For the establishment stage, ½ MS was determined to be the best culture medium. In the proliferation stage, the growth regulator balance that showed the highest survival rate was MS + IBA 1 ppm + BA 0.5 ppm. Different combinations of NAA-AIB were tested for rooting; MS + NAA 0.005 ppm + IBA 0.005 ppm was determined as the best medium. In the last stage, 65% of in vitro plants were acclimatized using vermiculite as substrate and irrigating with 50% Hoagland mineral solution.
author Medina Medina,Fiorella
Lazo Rodríguez,Herbert
author_facet Medina Medina,Fiorella
Lazo Rodríguez,Herbert
author_sort Medina Medina,Fiorella
title Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru
title_short Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru
title_full Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru
title_fullStr Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru
title_full_unstemmed Establishment of an in vitro micropropagation protocol for Cantua volcánica (Polemoniaceae): a potential ornamental plant of Peru
title_sort establishment of an in vitro micropropagation protocol for cantua volcánica (polemoniaceae): a potential ornamental plant of peru
publisher Universidad de Tarapacá. Facultad de Ciencias Agronómicas
publishDate 2018
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000200203
work_keys_str_mv AT medinamedinafiorella establishmentofaninvitromicropropagationprotocolforcantuavolcanicapolemoniaceaeapotentialornamentalplantofperu
AT lazorodriguezherbert establishmentofaninvitromicropropagationprotocolforcantuavolcanicapolemoniaceaeapotentialornamentalplantofperu
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