Rapid diagnostic PCR method for identification of the genera Sarcocornia and Salicornia

ABSTRACT Plants that belong to different genera sometimes may present close morphological similarity and cannot be distinguished phe notypically by non-specialists. The aim of this study was to develop a simple diagnostic PCR for the identification of plants of Sarcocornia and Salicornia and to test...

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Autores principales:	Contreras,Roberto, Sepúlveda,Bernardo, Aguayo,Fernanda, Porcile,Vincenzo
Lenguaje:	English
Publicado:	Universidad de Tarapacá. Facultad de Ciencias Agronómicas 2018
Materias:	Sarcocornia PCR allele-specific primer SNP
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000300095
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spelling	oai:scielo:S0718-342920180003000952019-04-04Rapid diagnostic PCR method for identification of the genera Sarcocornia and SalicorniaContreras,RobertoSepúlveda,BernardoAguayo,FernandaPorcile,Vincenzo Sarcocornia PCR allele-specific primer SNP ABSTRACT Plants that belong to different genera sometimes may present close morphological similarity and cannot be distinguished phe notypically by non-specialists. The aim of this study was to develop a simple diagnostic PCR for the identification of plants of Sarcocornia and Salicornia and to test this new procedure to identify 82 samples of Sarcocornia neii from coastal and valleys of the Atacama region of Chile. Six primer pairs were designed from ETS sequences of the genera Sarcocornia and Salicornia and evaluated for the identification of both genera. Primers with a mismatch in the 3’ nucleotide indicate the site of the SNP. Four primer pairs (SALI2F-2R, SALI3F-4R, SARCO1F-1R and SARCO3F-3R) were selected to develop an efficient and simple diagnostic PCR for the identification of Sarcocornia and Salicornia. The results show that with this method is possible to identify Sarcocornia and Salicornia. This method may be useful as an approach for genetic traceability of conserved products (sea asparagus). This work provides an applicable and efficient method using only DNA, PCR and electrophoresis.info:eu-repo/semantics/openAccessUniversidad de Tarapacá. Facultad de Ciencias AgronómicasIdesia (Arica) v.36 n.3 20182018-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000300095en10.4067/S0718-34292018005001501
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Sarcocornia PCR allele-specific primer SNP
spellingShingle	Sarcocornia PCR allele-specific primer SNP Contreras,Roberto Sepúlveda,Bernardo Aguayo,Fernanda Porcile,Vincenzo Rapid diagnostic PCR method for identification of the genera Sarcocornia and Salicornia
description	ABSTRACT Plants that belong to different genera sometimes may present close morphological similarity and cannot be distinguished phe notypically by non-specialists. The aim of this study was to develop a simple diagnostic PCR for the identification of plants of Sarcocornia and Salicornia and to test this new procedure to identify 82 samples of Sarcocornia neii from coastal and valleys of the Atacama region of Chile. Six primer pairs were designed from ETS sequences of the genera Sarcocornia and Salicornia and evaluated for the identification of both genera. Primers with a mismatch in the 3’ nucleotide indicate the site of the SNP. Four primer pairs (SALI2F-2R, SALI3F-4R, SARCO1F-1R and SARCO3F-3R) were selected to develop an efficient and simple diagnostic PCR for the identification of Sarcocornia and Salicornia. The results show that with this method is possible to identify Sarcocornia and Salicornia. This method may be useful as an approach for genetic traceability of conserved products (sea asparagus). This work provides an applicable and efficient method using only DNA, PCR and electrophoresis.
author	Contreras,Roberto Sepúlveda,Bernardo Aguayo,Fernanda Porcile,Vincenzo
author_facet	Contreras,Roberto Sepúlveda,Bernardo Aguayo,Fernanda Porcile,Vincenzo
author_sort	Contreras,Roberto
title	Rapid diagnostic PCR method for identification of the genera Sarcocornia and Salicornia
title_short	Rapid diagnostic PCR method for identification of the genera Sarcocornia and Salicornia
title_full	Rapid diagnostic PCR method for identification of the genera Sarcocornia and Salicornia
title_fullStr	Rapid diagnostic PCR method for identification of the genera Sarcocornia and Salicornia
title_full_unstemmed	Rapid diagnostic PCR method for identification of the genera Sarcocornia and Salicornia
title_sort	rapid diagnostic pcr method for identification of the genera sarcocornia and salicornia
publisher	Universidad de Tarapacá. Facultad de Ciencias Agronómicas
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292018000300095
work_keys_str_mv	AT contrerasroberto rapiddiagnosticpcrmethodforidentificationofthegenerasarcocorniaandsalicornia AT sepulvedabernardo rapiddiagnosticpcrmethodforidentificationofthegenerasarcocorniaandsalicornia AT aguayofernanda rapiddiagnosticpcrmethodforidentificationofthegenerasarcocorniaandsalicornia AT porcilevincenzo rapiddiagnosticpcrmethodforidentificationofthegenerasarcocorniaandsalicornia
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Rapid diagnostic PCR method for identification of the genera Sarcocornia and Salicornia

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