Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study

The disinfection of dental impression materials has become an essential topic of universal concern, as it may be the first instance of microbial contamination during dental care. The purpose of this study is twofold: To determine the effectiveness of disinfection with 0.5% sodium hypochlorite and 2%...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bustos,J, Herrera,R, González,U, Martínez,A, Catalán,A
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2010
Materias:	disinfection irreversible hydrocolloid silicone impression 0.5% sodium NaOCl 2% glutaraldehyde
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2010000200011
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-381X2010000200011
record_format	dspace
spelling	oai:scielo:S0718-381X20100002000112012-01-19Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM StudyBustos,JHerrera,RGonzález,UMartínez,ACatalán,A disinfection irreversible hydrocolloid silicone impression 0.5% sodium NaOCl 2% glutaraldehyde The disinfection of dental impression materials has become an essential topic of universal concern, as it may be the first instance of microbial contamination during dental care. The purpose of this study is twofold: To determine the effectiveness of disinfection with 0.5% sodium hypochlorite and 2% glutaraldehyde solutions in irreversible hydrocolloid (alginate) and silicone impressions and to analyze the effect of disinfection on surface quality with a scanning electronic microscope. A total of 32 impressions (16 made of irreversible hydrocolloid and 16 made of silicone) were taken from maxillary dentate patients. Samples of 1cm2 (80 , irreversible hydrocolloid, 80 silicone) were obtained and distributed in ten groups: alginate in no disinfectant (control group 1, AL), alginate in 0.5% sodium hypochlorite (NaOCl) for 5 (AH5) and 10 minutes (AH10), alginate in 2% glutaraldehyde for 5 (AG5) and 10 minutes (AG10), silicone in no disinfectant (control group 2), silicone in 0.5% NaOCl for 5 (SH5) and 10 minutes (SH10), and silicone in 2% glutaraldehyde for 5 (SG5) and 10 minutes (SG10). Each sample was divided into two segments (one for the microbiological and one for the SEM study). Microbiological samples were planted on blood agar, Mac Conkey agar, and Sabouraud agar, and identification was made by Gram´s stain. The samples were then processed by an SEM. Immersion in the 5% NaOCl and 2% glutaraldehyde for 10 minutes completely eliminated bacteria in the impressions, compared with the control group (P=0.000004). Immersion in 0.5% NaOCl and 2% glutaraldehyde for 5 and 10 minutes significantly inhibited bacterial growth in both the irreversible hydrocolloid and silicone impressions compared with control group (P<0.05). However, in the SEM study, immersion in both disinfectant solutions for 5 and 10 minutes did not significantly affect the surface quality of the irreversible hydrocolloid and silicone impressions compared with immersion in the non-disinfectant samples. Impression materials retain bacteria. Immersion in 0.5% NaOCl solution and 2% glutaraldehyde for 5 minutes can successfully disinfect irreversible hydrocolloid and silicone impressions. The results showed that it is prudent for either the operator or the technician to treat impressions for 5 minutes by immersion in 0.5% NaOCl or 2% glutaraldehyde to reduce the level of bacterial contamination and hence the risk of cross infection.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.4 n.2 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2010000200011en10.4067/S0718-381X2010000200011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	disinfection irreversible hydrocolloid silicone impression 0.5% sodium NaOCl 2% glutaraldehyde
spellingShingle	disinfection irreversible hydrocolloid silicone impression 0.5% sodium NaOCl 2% glutaraldehyde Bustos,J Herrera,R González,U Martínez,A Catalán,A Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study
description	The disinfection of dental impression materials has become an essential topic of universal concern, as it may be the first instance of microbial contamination during dental care. The purpose of this study is twofold: To determine the effectiveness of disinfection with 0.5% sodium hypochlorite and 2% glutaraldehyde solutions in irreversible hydrocolloid (alginate) and silicone impressions and to analyze the effect of disinfection on surface quality with a scanning electronic microscope. A total of 32 impressions (16 made of irreversible hydrocolloid and 16 made of silicone) were taken from maxillary dentate patients. Samples of 1cm2 (80 , irreversible hydrocolloid, 80 silicone) were obtained and distributed in ten groups: alginate in no disinfectant (control group 1, AL), alginate in 0.5% sodium hypochlorite (NaOCl) for 5 (AH5) and 10 minutes (AH10), alginate in 2% glutaraldehyde for 5 (AG5) and 10 minutes (AG10), silicone in no disinfectant (control group 2), silicone in 0.5% NaOCl for 5 (SH5) and 10 minutes (SH10), and silicone in 2% glutaraldehyde for 5 (SG5) and 10 minutes (SG10). Each sample was divided into two segments (one for the microbiological and one for the SEM study). Microbiological samples were planted on blood agar, Mac Conkey agar, and Sabouraud agar, and identification was made by Gram´s stain. The samples were then processed by an SEM. Immersion in the 5% NaOCl and 2% glutaraldehyde for 10 minutes completely eliminated bacteria in the impressions, compared with the control group (P=0.000004). Immersion in 0.5% NaOCl and 2% glutaraldehyde for 5 and 10 minutes significantly inhibited bacterial growth in both the irreversible hydrocolloid and silicone impressions compared with control group (P<0.05). However, in the SEM study, immersion in both disinfectant solutions for 5 and 10 minutes did not significantly affect the surface quality of the irreversible hydrocolloid and silicone impressions compared with immersion in the non-disinfectant samples. Impression materials retain bacteria. Immersion in 0.5% NaOCl solution and 2% glutaraldehyde for 5 minutes can successfully disinfect irreversible hydrocolloid and silicone impressions. The results showed that it is prudent for either the operator or the technician to treat impressions for 5 minutes by immersion in 0.5% NaOCl or 2% glutaraldehyde to reduce the level of bacterial contamination and hence the risk of cross infection.
author	Bustos,J Herrera,R González,U Martínez,A Catalán,A
author_facet	Bustos,J Herrera,R González,U Martínez,A Catalán,A
author_sort	Bustos,J
title	Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study
title_short	Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study
title_full	Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study
title_fullStr	Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study
title_full_unstemmed	Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study
title_sort	effect of inmersion desinfection with 0.5% sodium hypochlorite and 2% glutaraldehyde on alginate and silicone: microbiology and sem study
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2010000200011
work_keys_str_mv	AT bustosj effectofinmersiondesinfectionwith05sodiumhypochloriteand2glutaraldehydeonalginateandsiliconemicrobiologyandsemstudy AT herrerar effectofinmersiondesinfectionwith05sodiumhypochloriteand2glutaraldehydeonalginateandsiliconemicrobiologyandsemstudy AT gonzalezu effectofinmersiondesinfectionwith05sodiumhypochloriteand2glutaraldehydeonalginateandsiliconemicrobiologyandsemstudy AT martineza effectofinmersiondesinfectionwith05sodiumhypochloriteand2glutaraldehydeonalginateandsiliconemicrobiologyandsemstudy AT catalana effectofinmersiondesinfectionwith05sodiumhypochloriteand2glutaraldehydeonalginateandsiliconemicrobiologyandsemstudy
_version_	1714203917907132416

Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study

Ejemplares similares