Cytotoxicity of Dental Alginates

Alginate, or irreversible hydrocolloid, is one of the most accepted impression materials used in dentistry. However, some substances existing in these materials can be toxic. The aim of this study was to assess the cytotoxicity of alginates for dental applications. Fourteen different alginates were...

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Autores principales:	Pithon,Matheus Melo, dos Santos,Rogério Lacerda, Martins,Fernanda Otaviano, Romanos,Maria Teresa Villela
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2010
Materias:	cytotoxicity dental impression materials cell culture techniques
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2010000300015
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spelling	oai:scielo:S0718-381X20100003000152012-01-19Cytotoxicity of Dental AlginatesPithon,Matheus Melodos Santos,Rogério LacerdaMartins,Fernanda OtavianoRomanos,Maria Teresa Villela cytotoxicity dental impression materials cell culture techniques Alginate, or irreversible hydrocolloid, is one of the most accepted impression materials used in dentistry. However, some substances existing in these materials can be toxic. The aim of this study was to assess the cytotoxicity of alginates for dental applications. Fourteen different alginates were assessed: Jeltrate, Jeltrate Plus, Jeltrate Chromatic, Alga Gel, Printer Gel, Ava Gel, New Print, Kromopan 100, Tropicalgin, Cavex Orthotrace, Hydrogum, Orthoprint, Cavex Color Change, and Qualitygel. Three control groups were also used in this study: positive control group (C+) consisting of cell detergent Tween 80, negative control group (C-) consisting of PBS, and cell control group (CC) consisting of non-exposed cells. After manipulating the materials according to the manufacturer’s instructions, samples were made by using silicone rings. Next, the samples were immersed into Eagle’s minimum essential medium (MEM) for 2 minutes followed by removal of supernatants and contact with L929 fibroblasts. After contact with the medium, the cells were incubated for further 24 hours in which 100? l of 0.01% neutral red stain were added. Cells were incubated again for 3 hours so that the stain could be absorbed. After this period, the cells were fixed and viable cell counting was performed by using a spectrophotometer (BioTek, Winooski, Vermont, USA) at wavelength of 492 nm. The results demonstrated statistical differences between CC and C- groups in relation to other ones (p<0.05). No statistical differences were observed between Jeltrate Plus and Hydrogum groups, between Jeltrate and Jeltrate Chromatic, Printer Gel, Tropicalgin, and Qualitygel groups, and between Jeltrate Chromatic and Alga Gel, Ava Gel, New Print, Kromopan 100, Cavex Orthotrace, Hydrogum, Orhtoprint, and Cavex Color Change groups. One can conclude, based on the results of this study, that all alginate materials were found to be cytotoxic.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.4 n.3 20102010-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2010000300015en10.4067/S0718-381X2010000300015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cytotoxicity dental impression materials cell culture techniques
spellingShingle	cytotoxicity dental impression materials cell culture techniques Pithon,Matheus Melo dos Santos,Rogério Lacerda Martins,Fernanda Otaviano Romanos,Maria Teresa Villela Cytotoxicity of Dental Alginates
description	Alginate, or irreversible hydrocolloid, is one of the most accepted impression materials used in dentistry. However, some substances existing in these materials can be toxic. The aim of this study was to assess the cytotoxicity of alginates for dental applications. Fourteen different alginates were assessed: Jeltrate, Jeltrate Plus, Jeltrate Chromatic, Alga Gel, Printer Gel, Ava Gel, New Print, Kromopan 100, Tropicalgin, Cavex Orthotrace, Hydrogum, Orthoprint, Cavex Color Change, and Qualitygel. Three control groups were also used in this study: positive control group (C+) consisting of cell detergent Tween 80, negative control group (C-) consisting of PBS, and cell control group (CC) consisting of non-exposed cells. After manipulating the materials according to the manufacturer’s instructions, samples were made by using silicone rings. Next, the samples were immersed into Eagle’s minimum essential medium (MEM) for 2 minutes followed by removal of supernatants and contact with L929 fibroblasts. After contact with the medium, the cells were incubated for further 24 hours in which 100? l of 0.01% neutral red stain were added. Cells were incubated again for 3 hours so that the stain could be absorbed. After this period, the cells were fixed and viable cell counting was performed by using a spectrophotometer (BioTek, Winooski, Vermont, USA) at wavelength of 492 nm. The results demonstrated statistical differences between CC and C- groups in relation to other ones (p<0.05). No statistical differences were observed between Jeltrate Plus and Hydrogum groups, between Jeltrate and Jeltrate Chromatic, Printer Gel, Tropicalgin, and Qualitygel groups, and between Jeltrate Chromatic and Alga Gel, Ava Gel, New Print, Kromopan 100, Cavex Orthotrace, Hydrogum, Orhtoprint, and Cavex Color Change groups. One can conclude, based on the results of this study, that all alginate materials were found to be cytotoxic.
author	Pithon,Matheus Melo dos Santos,Rogério Lacerda Martins,Fernanda Otaviano Romanos,Maria Teresa Villela
author_facet	Pithon,Matheus Melo dos Santos,Rogério Lacerda Martins,Fernanda Otaviano Romanos,Maria Teresa Villela
author_sort	Pithon,Matheus Melo
title	Cytotoxicity of Dental Alginates
title_short	Cytotoxicity of Dental Alginates
title_full	Cytotoxicity of Dental Alginates
title_fullStr	Cytotoxicity of Dental Alginates
title_full_unstemmed	Cytotoxicity of Dental Alginates
title_sort	cytotoxicity of dental alginates
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2010000300015
work_keys_str_mv	AT pithonmatheusmelo cytotoxicityofdentalalginates AT dossantosrogeriolacerda cytotoxicityofdentalalginates AT martinsfernandaotaviano cytotoxicityofdentalalginates AT romanosmariateresavillela cytotoxicityofdentalalginates
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Cytotoxicity of Dental Alginates

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