Dissolution of Connective Tissue in Sodium Hypochlorite Alone and in Combination with 3% Hydrogen Peroxide

The aim was to evaluate the dissolution of porcine muscle using sodium hypochlorite in several concentrations and in combination with 3% hydrogen peroxide. Small pieces of porcine muscle were cut and their weight adjusted at 0.02g to be submerged in 1 ml of each test solution which was changed in 1...

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Autores principales: Echeverri,D, Acuña,C
Lenguaje:English
Publicado: Universidad de La Frontera. Facultad de Medicina 2012
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2012000300003
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spelling oai:scielo:S0718-381X20120003000032013-06-03Dissolution of Connective Tissue in Sodium Hypochlorite Alone and in Combination with 3% Hydrogen PeroxideEcheverri,DAcuña,C sodium hipochlorite irrigating solution hydrogen peroxide The aim was to evaluate the dissolution of porcine muscle using sodium hypochlorite in several concentrations and in combination with 3% hydrogen peroxide. Small pieces of porcine muscle were cut and their weight adjusted at 0.02g to be submerged in 1 ml of each test solution which was changed in 1 minute intervals until complete dissolution of the sample was observed. The 75 pieces of tissue were divided in 5 groups and different irrigant solutions were used as follows: Group 1, 5% sodium hypochlorite (NaOCl 5%). Group 2, 2.5% sodium hypochlorite (NaOCl 2.5%). Group 3, 2.5% sodium hypochlorite and 3% hydrogen peroxide alternately (NaOCl 2.5% + H2O2 3%). Group 4,3% hydrogen peroxide (H2O2), and group 5 used saline (NaCl 0.9%) as a negative control. For 5% sodium hypochlorite the mean time until complete dissolution of the sample was 17.5 minutes. The mean time for group 2 was 18.5, for group 3 was 64.6 minutes and for groups 4 and 5 there was no tissue dissolution after 120 minutes. To evaluate statistical significance ANOVA multi sample test was performed and Tukey post hoc test with significance level at P=0.05. Under the conditions of this study there were no significant differences with the use of sodium hypochlorite at 5% and 2.5% in terms of dissolution speed (P=0.6). The combination of sodium hypochlorite and hydrogen peroxide alternately caused a statistical significant delay in the dissolution of porcine muscle (P<0.05). The combination of sodium hypochlorite and hydrogen peroxide does not offer any benefit in terms of speed of dissolution of connective necrotic tissue.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.6 n.3 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2012000300003en10.4067/S0718-381X2012000300003
institution Scielo Chile
collection Scielo Chile
language English
topic sodium hipochlorite
irrigating solution
hydrogen peroxide
spellingShingle sodium hipochlorite
irrigating solution
hydrogen peroxide
Echeverri,D
Acuña,C
Dissolution of Connective Tissue in Sodium Hypochlorite Alone and in Combination with 3% Hydrogen Peroxide
description The aim was to evaluate the dissolution of porcine muscle using sodium hypochlorite in several concentrations and in combination with 3% hydrogen peroxide. Small pieces of porcine muscle were cut and their weight adjusted at 0.02g to be submerged in 1 ml of each test solution which was changed in 1 minute intervals until complete dissolution of the sample was observed. The 75 pieces of tissue were divided in 5 groups and different irrigant solutions were used as follows: Group 1, 5% sodium hypochlorite (NaOCl 5%). Group 2, 2.5% sodium hypochlorite (NaOCl 2.5%). Group 3, 2.5% sodium hypochlorite and 3% hydrogen peroxide alternately (NaOCl 2.5% + H2O2 3%). Group 4,3% hydrogen peroxide (H2O2), and group 5 used saline (NaCl 0.9%) as a negative control. For 5% sodium hypochlorite the mean time until complete dissolution of the sample was 17.5 minutes. The mean time for group 2 was 18.5, for group 3 was 64.6 minutes and for groups 4 and 5 there was no tissue dissolution after 120 minutes. To evaluate statistical significance ANOVA multi sample test was performed and Tukey post hoc test with significance level at P=0.05. Under the conditions of this study there were no significant differences with the use of sodium hypochlorite at 5% and 2.5% in terms of dissolution speed (P=0.6). The combination of sodium hypochlorite and hydrogen peroxide alternately caused a statistical significant delay in the dissolution of porcine muscle (P<0.05). The combination of sodium hypochlorite and hydrogen peroxide does not offer any benefit in terms of speed of dissolution of connective necrotic tissue.
author Echeverri,D
Acuña,C
author_facet Echeverri,D
Acuña,C
author_sort Echeverri,D
title Dissolution of Connective Tissue in Sodium Hypochlorite Alone and in Combination with 3% Hydrogen Peroxide
title_short Dissolution of Connective Tissue in Sodium Hypochlorite Alone and in Combination with 3% Hydrogen Peroxide
title_full Dissolution of Connective Tissue in Sodium Hypochlorite Alone and in Combination with 3% Hydrogen Peroxide
title_fullStr Dissolution of Connective Tissue in Sodium Hypochlorite Alone and in Combination with 3% Hydrogen Peroxide
title_full_unstemmed Dissolution of Connective Tissue in Sodium Hypochlorite Alone and in Combination with 3% Hydrogen Peroxide
title_sort dissolution of connective tissue in sodium hypochlorite alone and in combination with 3% hydrogen peroxide
publisher Universidad de La Frontera. Facultad de Medicina
publishDate 2012
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2012000300003
work_keys_str_mv AT echeverrid dissolutionofconnectivetissueinsodiumhypochloritealoneandincombinationwith3hydrogenperoxide
AT acunac dissolutionofconnectivetissueinsodiumhypochloritealoneandincombinationwith3hydrogenperoxide
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