In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite

The aim of this study was to observe whether the antibacterial effect of 2% chlorhexidine against Enterococcus faecalis in dentine is altered by previous irrigation with 5% sodium hypochlorite. Dentin discs were prepared with different irrigation protocols: group 1, immersed in 2% CHX for 25 min; gr...

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Autores principales:	Echeverri,Diego, Alderete,Daniela
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2015
Materias:	chlorhexidine digluconate sodium hypochlorite irrigant combination Enterococcus faecalis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2015000100004
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spelling	oai:scielo:S0718-381X20150001000042015-08-11In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium HypochloriteEcheverri,DiegoAlderete,Daniela chlorhexidine digluconate sodium hypochlorite irrigant combination Enterococcus faecalis The aim of this study was to observe whether the antibacterial effect of 2% chlorhexidine against Enterococcus faecalis in dentine is altered by previous irrigation with 5% sodium hypochlorite. Dentin discs were prepared with different irrigation protocols: group 1, immersed in 2% CHX for 25 min; group 2 immersed in 5% NaOCl for 25 minutes; group 3, immersed in 5% NaOCl, dried and irrigated with 3 ml of 2% CHX; group 4 and 5 immersed in 5% NaOCl, rinsed with 5 and 25 ml of distilled water respectively, dried and irrigated with 2% CHX. Group 6, immersed in 0.9% sodium chloride. Discs were then placed in agar plates in which E. faecalis was grown and the inhibition zone around each disc was measured after 24 hours of incubation at 37 C. All experimental groups showed E. faecalis growth inhibition. The most effective irrigant was 2% CHX (P<0.05). Groups in which both NaOCl and CHX were used displayed significantly smaller inhibition halos as compared with 2% CHX. Different volumes of water for rinsing did not cause significant improvement in growth inhibition. The antimicrobial effect of 2% chlorhexidine against E. faecalis was significantly reduced when dentin was previously irrigated with 5% sodium hypochlorite despite of rinsing with different volumes of water.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.9 n.1 20152015-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2015000100004en10.4067/S0718-381X2015000100004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	chlorhexidine digluconate sodium hypochlorite irrigant combination Enterococcus faecalis
spellingShingle	chlorhexidine digluconate sodium hypochlorite irrigant combination Enterococcus faecalis Echeverri,Diego Alderete,Daniela In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite
description	The aim of this study was to observe whether the antibacterial effect of 2% chlorhexidine against Enterococcus faecalis in dentine is altered by previous irrigation with 5% sodium hypochlorite. Dentin discs were prepared with different irrigation protocols: group 1, immersed in 2% CHX for 25 min; group 2 immersed in 5% NaOCl for 25 minutes; group 3, immersed in 5% NaOCl, dried and irrigated with 3 ml of 2% CHX; group 4 and 5 immersed in 5% NaOCl, rinsed with 5 and 25 ml of distilled water respectively, dried and irrigated with 2% CHX. Group 6, immersed in 0.9% sodium chloride. Discs were then placed in agar plates in which E. faecalis was grown and the inhibition zone around each disc was measured after 24 hours of incubation at 37 C. All experimental groups showed E. faecalis growth inhibition. The most effective irrigant was 2% CHX (P<0.05). Groups in which both NaOCl and CHX were used displayed significantly smaller inhibition halos as compared with 2% CHX. Different volumes of water for rinsing did not cause significant improvement in growth inhibition. The antimicrobial effect of 2% chlorhexidine against E. faecalis was significantly reduced when dentin was previously irrigated with 5% sodium hypochlorite despite of rinsing with different volumes of water.
author	Echeverri,Diego Alderete,Daniela
author_facet	Echeverri,Diego Alderete,Daniela
author_sort	Echeverri,Diego
title	In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite
title_short	In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite
title_full	In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite
title_fullStr	In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite
title_full_unstemmed	In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite
title_sort	in vitro antibacterial effect of 2% chlorhexidine against enterococcus faecalis in dentin previously irrigated with 5% sodium hypochlorite
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2015000100004
work_keys_str_mv	AT echeverridiego invitroantibacterialeffectof2chlorhexidineagainstenterococcusfaecalisindentinpreviouslyirrigatedwith5sodiumhypochlorite AT alderetedaniela invitroantibacterialeffectof2chlorhexidineagainstenterococcusfaecalisindentinpreviouslyirrigatedwith5sodiumhypochlorite
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In vitro Antibacterial Effect of 2% Chlorhexidine Against Enterococcus faecalis in Dentin Previously Irrigated with 5% Sodium Hypochlorite

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