Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root Canals

The incomplete disinfection of root canal system has been reported as the main cause of post-treatment disease, due to the persistence of bacteria. For over thirty years Enterococcus faecalis has been considered the most common bacterial species isolated from persistent root canal infections, resist...

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Autores principales:	Sánchez-Sanhueza,Gabriela, Alcántara-Dufeu,Raúl, Carrillo,Lorena, Mansilla,Hedring, Novoa,Camila, Bello-Toledo,Helia
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2015
Materias:	copper sulfate Enterococcus faecalis bacterial load endodontics
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2015000300024
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id	oai:scielo:S0718-381X2015000300024
record_format	dspace
spelling	oai:scielo:S0718-381X20150003000242016-03-22Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root CanalsSánchez-Sanhueza,GabrielaAlcántara-Dufeu,RaúlCarrillo,LorenaMansilla,HedringNovoa,CamilaBello-Toledo,Helia copper sulfate Enterococcus faecalis bacterial load endodontics The incomplete disinfection of root canal system has been reported as the main cause of post-treatment disease, due to the persistence of bacteria. For over thirty years Enterococcus faecalis has been considered the most common bacterial species isolated from persistent root canal infections, resisting antibacterial agents, such as chlorhexidine and calcium hydroxide. Several studies have indicated that copper has optimal disinfecting capacities in a hospital environment. Aimed to know the ex vivo effect of copper sulfate (CuSO4) on E. faecalis. Thirty-six extracted human tooth root canals were inoculated with E. faecalis ATCC 29212. These root canals had undergone endondontic procedures with a rotatory system. The effect of CuSO4 was determined by plate count method of E. faecalis obtained from one sample of each tooth under three incubation times (4th, 7th and 10th day). The canals medicated with CuSO4, the bacterial count decreased 6 log after 4 days and remained as such without statistically significant change until the tenth day. It is an indisputable fact of its antibacterial action. The bacterial persistence may be due to the ability of E. faecalis to remain viable in root canals up to 12 months without additional nutrients. These preliminary results couldbe used for further scientific work assessing the potential for the use of cooper in dentistry.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.9 n.3 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2015000300024en10.4067/S0718-381X2015000300024
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	copper sulfate Enterococcus faecalis bacterial load endodontics
spellingShingle	copper sulfate Enterococcus faecalis bacterial load endodontics Sánchez-Sanhueza,Gabriela Alcántara-Dufeu,Raúl Carrillo,Lorena Mansilla,Hedring Novoa,Camila Bello-Toledo,Helia Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root Canals
description	The incomplete disinfection of root canal system has been reported as the main cause of post-treatment disease, due to the persistence of bacteria. For over thirty years Enterococcus faecalis has been considered the most common bacterial species isolated from persistent root canal infections, resisting antibacterial agents, such as chlorhexidine and calcium hydroxide. Several studies have indicated that copper has optimal disinfecting capacities in a hospital environment. Aimed to know the ex vivo effect of copper sulfate (CuSO4) on E. faecalis. Thirty-six extracted human tooth root canals were inoculated with E. faecalis ATCC 29212. These root canals had undergone endondontic procedures with a rotatory system. The effect of CuSO4 was determined by plate count method of E. faecalis obtained from one sample of each tooth under three incubation times (4th, 7th and 10th day). The canals medicated with CuSO4, the bacterial count decreased 6 log after 4 days and remained as such without statistically significant change until the tenth day. It is an indisputable fact of its antibacterial action. The bacterial persistence may be due to the ability of E. faecalis to remain viable in root canals up to 12 months without additional nutrients. These preliminary results couldbe used for further scientific work assessing the potential for the use of cooper in dentistry.
author	Sánchez-Sanhueza,Gabriela Alcántara-Dufeu,Raúl Carrillo,Lorena Mansilla,Hedring Novoa,Camila Bello-Toledo,Helia
author_facet	Sánchez-Sanhueza,Gabriela Alcántara-Dufeu,Raúl Carrillo,Lorena Mansilla,Hedring Novoa,Camila Bello-Toledo,Helia
author_sort	Sánchez-Sanhueza,Gabriela
title	Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root Canals
title_short	Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root Canals
title_full	Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root Canals
title_fullStr	Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root Canals
title_full_unstemmed	Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root Canals
title_sort	ex vivo effect of copper sulfate on enterococcus faecalis in root canals
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2015000300024
work_keys_str_mv	AT sanchezsanhuezagabriela exvivoeffectofcoppersulfateonenterococcusfaecalisinrootcanals AT alcantaradufeuraul exvivoeffectofcoppersulfateonenterococcusfaecalisinrootcanals AT carrillolorena exvivoeffectofcoppersulfateonenterococcusfaecalisinrootcanals AT mansillahedring exvivoeffectofcoppersulfateonenterococcusfaecalisinrootcanals AT novoacamila exvivoeffectofcoppersulfateonenterococcusfaecalisinrootcanals AT bellotoledohelia exvivoeffectofcoppersulfateonenterococcusfaecalisinrootcanals
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Ex vivo Effect of Copper Sulfate on Enterococcus faecalis in Root Canals

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