Sucrose Induced Dentin Demineralization in a Microcosm Biofilm Model

The aim of this in vitro study was to assess the root dentin demineralization caused by a microcosm biofilm model that has been exposed to sucrose in different ways. Materials and Methods: Saliva of two volunteers was inoculated into an artificial medium for biofilm growth and dentin blocks were imm...

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Autores principales:	Bohn,Ana Clarissa Cavalcante Elvas, Vale,Glauber Campos, Padovani,Gislane Cristina, Rodrigues,Lidiany Karla Azevedo, Prado-Júnior,Raimundo Rosendo
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2017
Materias:	biofilm dentin sucrose polysaccharides
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2017000100017
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spelling	oai:scielo:S0718-381X20170001000172017-05-09Sucrose Induced Dentin Demineralization in a Microcosm Biofilm ModelBohn,Ana Clarissa Cavalcante ElvasVale,Glauber CamposPadovani,Gislane CristinaRodrigues,Lidiany Karla AzevedoPrado-Júnior,Raimundo Rosendo biofilm dentin sucrose polysaccharides The aim of this in vitro study was to assess the root dentin demineralization caused by a microcosm biofilm model that has been exposed to sucrose in different ways. Materials and Methods: Saliva of two volunteers was inoculated into an artificial medium for biofilm growth and dentin blocks were immersed into these media. Dentin specimens were randomly exposed to one of the five experimental conditions: C (control group - no saliva inoculum or sucrose), 0S (saliva inoculum without sucrose, negative control), 3S (three daily one-minute immersions in 20 % sucrose), 6S (six daily one-minute immersions in 20 % sucrose), and CS (continuously immersed in 5 % sucrose). After five days, biofilm was collected to determine the concentration of intracellular and extracellular polysaccharides and the dentin surface hardness loss (SHL) was measured. The experiment was carried out in triplicate. Results: The dentin SHL was higher in groups that were exposed to sucrose (3S, 6S and CS) and there was a statistically significant difference between all groups (p<0.001). CS had higher concentrations of polysaccharides (p>0.001) and there was no statistically significant difference between the other groups (0S, 3S and 6S) (p>0.005). Conclusion: The microcosm biofilm model developed has the potential to produce root dentin demineralization at different exposures to sucrose.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.11 n.1 20172017-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2017000100017en10.4067/S0718-381X2017000100017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biofilm dentin sucrose polysaccharides
spellingShingle	biofilm dentin sucrose polysaccharides Bohn,Ana Clarissa Cavalcante Elvas Vale,Glauber Campos Padovani,Gislane Cristina Rodrigues,Lidiany Karla Azevedo Prado-Júnior,Raimundo Rosendo Sucrose Induced Dentin Demineralization in a Microcosm Biofilm Model
description	The aim of this in vitro study was to assess the root dentin demineralization caused by a microcosm biofilm model that has been exposed to sucrose in different ways. Materials and Methods: Saliva of two volunteers was inoculated into an artificial medium for biofilm growth and dentin blocks were immersed into these media. Dentin specimens were randomly exposed to one of the five experimental conditions: C (control group - no saliva inoculum or sucrose), 0S (saliva inoculum without sucrose, negative control), 3S (three daily one-minute immersions in 20 % sucrose), 6S (six daily one-minute immersions in 20 % sucrose), and CS (continuously immersed in 5 % sucrose). After five days, biofilm was collected to determine the concentration of intracellular and extracellular polysaccharides and the dentin surface hardness loss (SHL) was measured. The experiment was carried out in triplicate. Results: The dentin SHL was higher in groups that were exposed to sucrose (3S, 6S and CS) and there was a statistically significant difference between all groups (p<0.001). CS had higher concentrations of polysaccharides (p>0.001) and there was no statistically significant difference between the other groups (0S, 3S and 6S) (p>0.005). Conclusion: The microcosm biofilm model developed has the potential to produce root dentin demineralization at different exposures to sucrose.
author	Bohn,Ana Clarissa Cavalcante Elvas Vale,Glauber Campos Padovani,Gislane Cristina Rodrigues,Lidiany Karla Azevedo Prado-Júnior,Raimundo Rosendo
author_facet	Bohn,Ana Clarissa Cavalcante Elvas Vale,Glauber Campos Padovani,Gislane Cristina Rodrigues,Lidiany Karla Azevedo Prado-Júnior,Raimundo Rosendo
author_sort	Bohn,Ana Clarissa Cavalcante Elvas
title	Sucrose Induced Dentin Demineralization in a Microcosm Biofilm Model
title_short	Sucrose Induced Dentin Demineralization in a Microcosm Biofilm Model
title_full	Sucrose Induced Dentin Demineralization in a Microcosm Biofilm Model
title_fullStr	Sucrose Induced Dentin Demineralization in a Microcosm Biofilm Model
title_full_unstemmed	Sucrose Induced Dentin Demineralization in a Microcosm Biofilm Model
title_sort	sucrose induced dentin demineralization in a microcosm biofilm model
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2017000100017
work_keys_str_mv	AT bohnanaclarissacavalcanteelvas sucroseinduceddentindemineralizationinamicrocosmbiofilmmodel AT valeglaubercampos sucroseinduceddentindemineralizationinamicrocosmbiofilmmodel AT padovanigislanecristina sucroseinduceddentindemineralizationinamicrocosmbiofilmmodel AT rodrigueslidianykarlaazevedo sucroseinduceddentindemineralizationinamicrocosmbiofilmmodel AT pradojuniorraimundorosendo sucroseinduceddentindemineralizationinamicrocosmbiofilmmodel
_version_	1714203990257827840

Sucrose Induced Dentin Demineralization in a Microcosm Biofilm Model

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