Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions

The aim of this study was to evaluate the extraction of dental DNA exposed to different chemical solutions. The sample was composed by 15 subjects, from which 5 samples of oral mucosal cells (reference population) and 15 teeth (experimental population) were collected. The experimental population was...

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Autores principales:	Damascena,Nicole Prata, Santos Filho,Moezio de Vasconcellos Costa, de Souza,Gustavo Reis Branco, da Silva,Luiz Antonio Ferreira, Estevam,Charles dos Santos, Franco,Ademir, Paranhos,Luiz Renato, Musse,Jamilly de Oliveira
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2017
Materias:	forensic sciences forensic dentistry tooth DNA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2017000200009
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id	oai:scielo:S0718-381X2017000200009
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spelling	oai:scielo:S0718-381X20170002000092017-07-24Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical SolutionsDamascena,Nicole PrataSantos Filho,Moezio de Vasconcellos Costade Souza,Gustavo Reis Brancoda Silva,Luiz Antonio FerreiraEstevam,Charles dos SantosFranco,AdemirParanhos,Luiz RenatoMusse,Jamilly de Oliveira forensic sciences forensic dentistry tooth DNA The aim of this study was to evaluate the extraction of dental DNA exposed to different chemical solutions. The sample was composed by 15 subjects, from which 5 samples of oral mucosal cells (reference population) and 15 teeth (experimental population) were collected. The experimental population was divided in three equal parts, which were exposed to different chemical solutions, namely hydrochloric acid (HCl) at 37 %, formaldehyde (CH2O) at 10 % and sodium hypochlorite (NaOCl) at 2.5 %. The DNA from the oral mucosa was extracted using organic method, while the dental DNA was extracted using the AFDIL method, including amplification by PCR and sequencing through capillary electrophoresis. The DNA exposed to hydrochloric acid dissolved, lacking extraction. The exposure of teeth to formaldehyde and sodium hypochlorite did not interfere in the extraction of DNA, once the amplification was visible in both experimental populations. The present outcomes demonstrated that DNA extraction may be limited under exposure to chemical solutions.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.11 n.2 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2017000200009en10.4067/S0718-381X2017000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	forensic sciences forensic dentistry tooth DNA
spellingShingle	forensic sciences forensic dentistry tooth DNA Damascena,Nicole Prata Santos Filho,Moezio de Vasconcellos Costa de Souza,Gustavo Reis Branco da Silva,Luiz Antonio Ferreira Estevam,Charles dos Santos Franco,Ademir Paranhos,Luiz Renato Musse,Jamilly de Oliveira Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions
description	The aim of this study was to evaluate the extraction of dental DNA exposed to different chemical solutions. The sample was composed by 15 subjects, from which 5 samples of oral mucosal cells (reference population) and 15 teeth (experimental population) were collected. The experimental population was divided in three equal parts, which were exposed to different chemical solutions, namely hydrochloric acid (HCl) at 37 %, formaldehyde (CH2O) at 10 % and sodium hypochlorite (NaOCl) at 2.5 %. The DNA from the oral mucosa was extracted using organic method, while the dental DNA was extracted using the AFDIL method, including amplification by PCR and sequencing through capillary electrophoresis. The DNA exposed to hydrochloric acid dissolved, lacking extraction. The exposure of teeth to formaldehyde and sodium hypochlorite did not interfere in the extraction of DNA, once the amplification was visible in both experimental populations. The present outcomes demonstrated that DNA extraction may be limited under exposure to chemical solutions.
author	Damascena,Nicole Prata Santos Filho,Moezio de Vasconcellos Costa de Souza,Gustavo Reis Branco da Silva,Luiz Antonio Ferreira Estevam,Charles dos Santos Franco,Ademir Paranhos,Luiz Renato Musse,Jamilly de Oliveira
author_facet	Damascena,Nicole Prata Santos Filho,Moezio de Vasconcellos Costa de Souza,Gustavo Reis Branco da Silva,Luiz Antonio Ferreira Estevam,Charles dos Santos Franco,Ademir Paranhos,Luiz Renato Musse,Jamilly de Oliveira
author_sort	Damascena,Nicole Prata
title	Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions
title_short	Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions
title_full	Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions
title_fullStr	Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions
title_full_unstemmed	Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions
title_sort	testing the extraction of dna from human teeth exposed to different chemical solutions
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2017000200009
work_keys_str_mv	AT damascenanicoleprata testingtheextractionofdnafromhumanteethexposedtodifferentchemicalsolutions AT santosfilhomoeziodevasconcelloscosta testingtheextractionofdnafromhumanteethexposedtodifferentchemicalsolutions AT desouzagustavoreisbranco testingtheextractionofdnafromhumanteethexposedtodifferentchemicalsolutions AT dasilvaluizantonioferreira testingtheextractionofdnafromhumanteethexposedtodifferentchemicalsolutions AT estevamcharlesdossantos testingtheextractionofdnafromhumanteethexposedtodifferentchemicalsolutions AT francoademir testingtheextractionofdnafromhumanteethexposedtodifferentchemicalsolutions AT paranhosluizrenato testingtheextractionofdnafromhumanteethexposedtodifferentchemicalsolutions AT mussejamillydeoliveira testingtheextractionofdnafromhumanteethexposedtodifferentchemicalsolutions
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Testing the Extraction of DNA from Human Teeth Exposed to Different Chemical Solutions

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