Molecular Typing of Enterococcus faecalis from Persistent Endodontic Infections

ABSTRACT: Molecular techniques that provide valuable information about the epidemiology of oral strains. The purpose of this study was to determine the genetic relatedness of 83 Enterococcus faecalis strains isolated from treated root canals. These strains were obtained from patients who were treate...

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Autores principales:	Sánchez-Sanhueza,Gabriela, Villegas,Catherine, Ulloa,Mª Ignacia, Pineda,Carla, González-Rocha,Gerardo, Domínguez,Mariana, Bello-Toledo,Helia
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2018
Materias:	Enterococcus faecalis pulsed field gel electrophoresis apical periodontitis nonsuppurative.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2018000100113
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spelling	oai:scielo:S0718-381X20180001001132018-05-03Molecular Typing of Enterococcus faecalis from Persistent Endodontic InfectionsSánchez-Sanhueza,GabrielaVillegas,CatherineUlloa,Mª IgnaciaPineda,CarlaGonzález-Rocha,GerardoDomínguez,MarianaBello-Toledo,Helia Enterococcus faecalis pulsed field gel electrophoresis apical periodontitis nonsuppurative. ABSTRACT: Molecular techniques that provide valuable information about the epidemiology of oral strains. The purpose of this study was to determine the genetic relatedness of 83 Enterococcus faecalis strains isolated from treated root canals. These strains were obtained from patients who were treated for persistent endodontic infections. E. faecalis isolates were molecular typed by Pulsed Field Gel Electrophoresis using Smal. Ten clonal groups and 13 pulse types with 38.7 % similarity for the less related strains were identified. Genetic heterogeneity among strains from different patients and a high level of genetic homogeneity among intrapatient strains were observed. Therefore, restriction endonuclease fingerprinting of genomic DNA from E. faecalis strains confirmed the polyclonality of the isolates obtained from the root canals of patients diagnosed with persistent endodontic infections, compared with other reports. These results provide additional data for a better understanding of the epidemiological aspects of root canal infections by E. faecalis.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.12 n.1 20182018-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2018000100113en10.4067/S0718-381X2018000100113
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Enterococcus faecalis pulsed field gel electrophoresis apical periodontitis nonsuppurative.
spellingShingle	Enterococcus faecalis pulsed field gel electrophoresis apical periodontitis nonsuppurative. Sánchez-Sanhueza,Gabriela Villegas,Catherine Ulloa,Mª Ignacia Pineda,Carla González-Rocha,Gerardo Domínguez,Mariana Bello-Toledo,Helia Molecular Typing of Enterococcus faecalis from Persistent Endodontic Infections
description	ABSTRACT: Molecular techniques that provide valuable information about the epidemiology of oral strains. The purpose of this study was to determine the genetic relatedness of 83 Enterococcus faecalis strains isolated from treated root canals. These strains were obtained from patients who were treated for persistent endodontic infections. E. faecalis isolates were molecular typed by Pulsed Field Gel Electrophoresis using Smal. Ten clonal groups and 13 pulse types with 38.7 % similarity for the less related strains were identified. Genetic heterogeneity among strains from different patients and a high level of genetic homogeneity among intrapatient strains were observed. Therefore, restriction endonuclease fingerprinting of genomic DNA from E. faecalis strains confirmed the polyclonality of the isolates obtained from the root canals of patients diagnosed with persistent endodontic infections, compared with other reports. These results provide additional data for a better understanding of the epidemiological aspects of root canal infections by E. faecalis.
author	Sánchez-Sanhueza,Gabriela Villegas,Catherine Ulloa,Mª Ignacia Pineda,Carla González-Rocha,Gerardo Domínguez,Mariana Bello-Toledo,Helia
author_facet	Sánchez-Sanhueza,Gabriela Villegas,Catherine Ulloa,Mª Ignacia Pineda,Carla González-Rocha,Gerardo Domínguez,Mariana Bello-Toledo,Helia
author_sort	Sánchez-Sanhueza,Gabriela
title	Molecular Typing of Enterococcus faecalis from Persistent Endodontic Infections
title_short	Molecular Typing of Enterococcus faecalis from Persistent Endodontic Infections
title_full	Molecular Typing of Enterococcus faecalis from Persistent Endodontic Infections
title_fullStr	Molecular Typing of Enterococcus faecalis from Persistent Endodontic Infections
title_full_unstemmed	Molecular Typing of Enterococcus faecalis from Persistent Endodontic Infections
title_sort	molecular typing of enterococcus faecalis from persistent endodontic infections
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2018000100113
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Molecular Typing of Enterococcus faecalis from Persistent Endodontic Infections

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