Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects

ABSTRACT: Periodontium can submit changes that lead to loss of integrity, such as periodontal disease, immune disorders or traumatic brushing. One of the most common consequences resulting from these events is the apical migration of gingival marginal tissue. Among biomaterials used for periodontal...

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Autores principales:	Merizalde,Gabriel, Lopera,Daniela, Villegas,Mauricio, Restrepo,Luz Marina
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2019
Materias:	fibrin periodontium fibroblasts wound healing gingival recession
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2019000100023
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spelling	oai:scielo:S0718-381X20190001000232020-01-16Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession DefectsMerizalde,GabrielLopera,DanielaVillegas,MauricioRestrepo,Luz Marina fibrin periodontium fibroblasts wound healing gingival recession ABSTRACT: Periodontium can submit changes that lead to loss of integrity, such as periodontal disease, immune disorders or traumatic brushing. One of the most common consequences resulting from these events is the apical migration of gingival marginal tissue. Among biomaterials used for periodontal tissue regeneration, fibrin matrices have received significant attention to correct gingival recessions. Five oral mucosa biopsies were extracted, fibroblasts were in vitro cultured and frozen in liquid nitrogen. Three 10 mL glass sterile tubes were filled with patient blood and centrifuged immediately; clots were extracted and compressed to obtain L-PRF membranes. Autologous oral mucosa fibroblasts were added to the membranes and surgical procedures were performed in five patients. L-PRF fibrin network pore size was too small to allow human fibroblasts penetration but they were firmly attached to membrane surface. Gingival fibroblasts from fresh cell culture and recently thawed were used to attach on the L-PRF membranes. It was possible to establish a protocol for blood collection, centrifugation, fibrin clot compression, fibroblast adhesion to the membrane surface and patient application in a relatively short time (1 hour-1 hour and 30 minutes). Two patients expressed pain symptoms and the other ones presented light swelling without pain. In the first week, adjacent tissue showed few inflammation signs. Research efforts are being conducted to develop more conservative surgical techniques and new biomaterials that can promote cellular proliferation. Because of its properties, L-PRF membranes represent a tempting alternative. A combined technique to treat adjacent recession defects with L-PRF membranes and autologous oral mucosa fibroblasts in a coronal displaced flap did not show initial advantage compared with a gold standard surgery that includes an autologous soft tissue graft. Nevertheless, it could be an alternative for clinical application as a new functional cell biomaterial. More clinical evidence is needed.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.13 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2019000100023en10.4067/S0718-381X2019000100023
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	fibrin periodontium fibroblasts wound healing gingival recession
spellingShingle	fibrin periodontium fibroblasts wound healing gingival recession Merizalde,Gabriel Lopera,Daniela Villegas,Mauricio Restrepo,Luz Marina Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects
description	ABSTRACT: Periodontium can submit changes that lead to loss of integrity, such as periodontal disease, immune disorders or traumatic brushing. One of the most common consequences resulting from these events is the apical migration of gingival marginal tissue. Among biomaterials used for periodontal tissue regeneration, fibrin matrices have received significant attention to correct gingival recessions. Five oral mucosa biopsies were extracted, fibroblasts were in vitro cultured and frozen in liquid nitrogen. Three 10 mL glass sterile tubes were filled with patient blood and centrifuged immediately; clots were extracted and compressed to obtain L-PRF membranes. Autologous oral mucosa fibroblasts were added to the membranes and surgical procedures were performed in five patients. L-PRF fibrin network pore size was too small to allow human fibroblasts penetration but they were firmly attached to membrane surface. Gingival fibroblasts from fresh cell culture and recently thawed were used to attach on the L-PRF membranes. It was possible to establish a protocol for blood collection, centrifugation, fibrin clot compression, fibroblast adhesion to the membrane surface and patient application in a relatively short time (1 hour-1 hour and 30 minutes). Two patients expressed pain symptoms and the other ones presented light swelling without pain. In the first week, adjacent tissue showed few inflammation signs. Research efforts are being conducted to develop more conservative surgical techniques and new biomaterials that can promote cellular proliferation. Because of its properties, L-PRF membranes represent a tempting alternative. A combined technique to treat adjacent recession defects with L-PRF membranes and autologous oral mucosa fibroblasts in a coronal displaced flap did not show initial advantage compared with a gold standard surgery that includes an autologous soft tissue graft. Nevertheless, it could be an alternative for clinical application as a new functional cell biomaterial. More clinical evidence is needed.
author	Merizalde,Gabriel Lopera,Daniela Villegas,Mauricio Restrepo,Luz Marina
author_facet	Merizalde,Gabriel Lopera,Daniela Villegas,Mauricio Restrepo,Luz Marina
author_sort	Merizalde,Gabriel
title	Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects
title_short	Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects
title_full	Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects
title_fullStr	Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects
title_full_unstemmed	Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects
title_sort	leucocyte platelet rich fibrin with autologous gingival fibroblasts in the treatment of adjacent recession defects
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2019000100023
work_keys_str_mv	AT merizaldegabriel leucocyteplateletrichfibrinwithautologousgingivalfibroblastsinthetreatmentofadjacentrecessiondefects AT loperadaniela leucocyteplateletrichfibrinwithautologousgingivalfibroblastsinthetreatmentofadjacentrecessiondefects AT villegasmauricio leucocyteplateletrichfibrinwithautologousgingivalfibroblastsinthetreatmentofadjacentrecessiondefects AT restrepoluzmarina leucocyteplateletrichfibrinwithautologousgingivalfibroblastsinthetreatmentofadjacentrecessiondefects
_version_	1714204012149997568

Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects

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