Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines

ABSTRACT: The contamination of the dental units’ waterlines is a reality, which can develop individual and collective disorders. The aim of this study was to evaluate the prevalence and resistance profile of bacteria on the internal surfaces of waterlines in a dental clinic from a Dentistr...

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Autores principales:	Figueiredo-Filho,Altamir Oliveira de, Bem,Jessica Silva Peixoto, Weber Sobrinho,Carlos Roberto, Souza,Fábio Barbosa de
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2019
Materias:	water biofilms equipment contamination infection control
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2019000300357
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spelling	oai:scielo:S0718-381X20190003003572019-08-09Microbiological Water Evaluation from Biofilm Adhered to Dental Unit WaterlinesFigueiredo-Filho,Altamir Oliveira deBem,Jessica Silva PeixotoWeber Sobrinho,Carlos RobertoSouza,Fábio Barbosa de water biofilms equipment contamination infection control ABSTRACT: The contamination of the dental units’ waterlines is a reality, which can develop individual and collective disorders. The aim of this study was to evaluate the prevalence and resistance profile of bacteria on the internal surfaces of waterlines in a dental clinic from a Dentistry school of a Brazilian university. The design was an exploratory, descriptive study with quantitative and qualitative approach. Samples (n=4) were collected for analysis at different points: the portion closest to the water reservoir of the chair, and the portion closest to the triple syringe. After collection the samples were cultured in BHI medium in an oven for 24-48 h at 37 °C. For the quantitative analysis 1 ml of each sample was used for serial dilution up to the dilution value seven. The colonies were counted after pour plate and the results expressed in UFC/cm2. The qualitative analysis was initiated with the cultivation of Agar Blood, EMB-Levine and Cetrimide Agar for 24 h, and the identification of bacteria was performed by microscopic analyses. The resistance profile was verified by classical antibiogram. The internal surfaces of unit waterlines units exhibited a mean of 2.44 x 109 CFU / cm2. Bacillus subtilis and Pseudomonas aeruginosa were identified. The resistance profile of Pseudomonas aeruginosa indicated sensitivity to all tested antibiotics. A large number of microorganisms was quantified from biofilm accumulated in the dental units’ waterlines. However, they were not resistant to classic antibiogram. Better management and application of decontamination protocols for waterlines need to be applied since opportunistic infections may be associated with Pseudomonas aeruginosa.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.13 n.3 20192019-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2019000300357en10.4067/S0718-381X2019000300357
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	water biofilms equipment contamination infection control
spellingShingle	water biofilms equipment contamination infection control Figueiredo-Filho,Altamir Oliveira de Bem,Jessica Silva Peixoto Weber Sobrinho,Carlos Roberto Souza,Fábio Barbosa de Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines
description	ABSTRACT: The contamination of the dental units’ waterlines is a reality, which can develop individual and collective disorders. The aim of this study was to evaluate the prevalence and resistance profile of bacteria on the internal surfaces of waterlines in a dental clinic from a Dentistry school of a Brazilian university. The design was an exploratory, descriptive study with quantitative and qualitative approach. Samples (n=4) were collected for analysis at different points: the portion closest to the water reservoir of the chair, and the portion closest to the triple syringe. After collection the samples were cultured in BHI medium in an oven for 24-48 h at 37 °C. For the quantitative analysis 1 ml of each sample was used for serial dilution up to the dilution value seven. The colonies were counted after pour plate and the results expressed in UFC/cm2. The qualitative analysis was initiated with the cultivation of Agar Blood, EMB-Levine and Cetrimide Agar for 24 h, and the identification of bacteria was performed by microscopic analyses. The resistance profile was verified by classical antibiogram. The internal surfaces of unit waterlines units exhibited a mean of 2.44 x 109 CFU / cm2. Bacillus subtilis and Pseudomonas aeruginosa were identified. The resistance profile of Pseudomonas aeruginosa indicated sensitivity to all tested antibiotics. A large number of microorganisms was quantified from biofilm accumulated in the dental units’ waterlines. However, they were not resistant to classic antibiogram. Better management and application of decontamination protocols for waterlines need to be applied since opportunistic infections may be associated with Pseudomonas aeruginosa.
author	Figueiredo-Filho,Altamir Oliveira de Bem,Jessica Silva Peixoto Weber Sobrinho,Carlos Roberto Souza,Fábio Barbosa de
author_facet	Figueiredo-Filho,Altamir Oliveira de Bem,Jessica Silva Peixoto Weber Sobrinho,Carlos Roberto Souza,Fábio Barbosa de
author_sort	Figueiredo-Filho,Altamir Oliveira de
title	Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines
title_short	Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines
title_full	Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines
title_fullStr	Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines
title_full_unstemmed	Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines
title_sort	microbiological water evaluation from biofilm adhered to dental unit waterlines
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2019000300357
work_keys_str_mv	AT figueiredofilhoaltamiroliveirade microbiologicalwaterevaluationfrombiofilmadheredtodentalunitwaterlines AT bemjessicasilvapeixoto microbiologicalwaterevaluationfrombiofilmadheredtodentalunitwaterlines AT webersobrinhocarlosroberto microbiologicalwaterevaluationfrombiofilmadheredtodentalunitwaterlines AT souzafabiobarbosade microbiologicalwaterevaluationfrombiofilmadheredtodentalunitwaterlines
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Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines

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