Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic Infections

ABSTRACT: Enterococci are important nosocomial pathogens due to their intrinsic multiresistance and the acquisition of new antibiotic resistance genes (ARG). Enterococcus faecalis has been shown to be one of the main pathogens in persistent endodontic infections, therefore, the main objective of thi...

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Autores principales:	Seguel,Natalia, Quezada-Aguiluz,Mario, González-Rocha,Gerardo, Bello-Toledo,Helia, Sánchez-Sanhueza,Gabriela
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2020
Materias:	E. faecalis antibiotic resistance persistent endodontic lesions antibiotic resistance genes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2020000300448
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spelling	oai:scielo:S0718-381X20200003004482020-05-27Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic InfectionsSeguel,NataliaQuezada-Aguiluz,MarioGonzález-Rocha,GerardoBello-Toledo,HeliaSánchez-Sanhueza,Gabriela E. faecalis antibiotic resistance persistent endodontic lesions antibiotic resistance genes ABSTRACT: Enterococci are important nosocomial pathogens due to their intrinsic multiresistance and the acquisition of new antibiotic resistance genes (ARG). Enterococcus faecalis has been shown to be one of the main pathogens in persistent endodontic infections, therefore, the main objective of this study was to evaluate the phenotype and resistance genotype of strains of E. faecalis isolated from teeth with persistent endodontic lesions, to the most commonly prescribed antibiotics in dentistry. Thirteen strains of E. faecalis of different pulsotype were analyzed to evaluate the susceptibility to antibiotics, amoxicillin, amoxicillin/clavulanic acid, tetracycline, erythromycin and metronidazole, using the Epsilometer test (E- test) and the presence of beta-lactamases with nitrocefin test. Finally, the detection of ARG was performed with a molecular polymerase chain reaction (PCR) technique and confirmed by the sequencing of the amplification products. Fisher's exact test was used, using 95 % confidence. Regarding the phenotype of resistance, the evaluated strains, independent of the pulsotype, were totally resistant to the action of metronidazole. Antibiotics with higher minimum inhibitory concentration (MIC) after metronidazole include tetracycline and erythromycin. In contrast, lower MIC are applied to the combination of amoxicillin with clavulanic acid. The nitrocefin test was positive only in one strain. Genotypically, two genetically distant strains isolated from a single patient, presented a genotype of resistance to erythromycin, determined by the presence of the ermB gene. No statistically significant relationship was found between phenotypic resistance and the presence of ARG in relation to erythromycin (p> 0.05). It was concluded that isolates of E. faecalis from persistent endodontic infections showed phenotypes of resistance to several antimicrobial agents, all of which were susceptible to amoxicillin/clavulanic acid. Periodic evaluation of susceptibility to antibiotics is suggested as an important practice for the surveillance of antibiotic resistance in oral strains.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.14 n.3 20202020-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2020000300448en10.4067/S0718-381X2020000300448
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	E. faecalis antibiotic resistance persistent endodontic lesions antibiotic resistance genes
spellingShingle	E. faecalis antibiotic resistance persistent endodontic lesions antibiotic resistance genes Seguel,Natalia Quezada-Aguiluz,Mario González-Rocha,Gerardo Bello-Toledo,Helia Sánchez-Sanhueza,Gabriela Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic Infections
description	ABSTRACT: Enterococci are important nosocomial pathogens due to their intrinsic multiresistance and the acquisition of new antibiotic resistance genes (ARG). Enterococcus faecalis has been shown to be one of the main pathogens in persistent endodontic infections, therefore, the main objective of this study was to evaluate the phenotype and resistance genotype of strains of E. faecalis isolated from teeth with persistent endodontic lesions, to the most commonly prescribed antibiotics in dentistry. Thirteen strains of E. faecalis of different pulsotype were analyzed to evaluate the susceptibility to antibiotics, amoxicillin, amoxicillin/clavulanic acid, tetracycline, erythromycin and metronidazole, using the Epsilometer test (E- test) and the presence of beta-lactamases with nitrocefin test. Finally, the detection of ARG was performed with a molecular polymerase chain reaction (PCR) technique and confirmed by the sequencing of the amplification products. Fisher's exact test was used, using 95 % confidence. Regarding the phenotype of resistance, the evaluated strains, independent of the pulsotype, were totally resistant to the action of metronidazole. Antibiotics with higher minimum inhibitory concentration (MIC) after metronidazole include tetracycline and erythromycin. In contrast, lower MIC are applied to the combination of amoxicillin with clavulanic acid. The nitrocefin test was positive only in one strain. Genotypically, two genetically distant strains isolated from a single patient, presented a genotype of resistance to erythromycin, determined by the presence of the ermB gene. No statistically significant relationship was found between phenotypic resistance and the presence of ARG in relation to erythromycin (p> 0.05). It was concluded that isolates of E. faecalis from persistent endodontic infections showed phenotypes of resistance to several antimicrobial agents, all of which were susceptible to amoxicillin/clavulanic acid. Periodic evaluation of susceptibility to antibiotics is suggested as an important practice for the surveillance of antibiotic resistance in oral strains.
author	Seguel,Natalia Quezada-Aguiluz,Mario González-Rocha,Gerardo Bello-Toledo,Helia Sánchez-Sanhueza,Gabriela
author_facet	Seguel,Natalia Quezada-Aguiluz,Mario González-Rocha,Gerardo Bello-Toledo,Helia Sánchez-Sanhueza,Gabriela
author_sort	Seguel,Natalia
title	Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic Infections
title_short	Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic Infections
title_full	Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic Infections
title_fullStr	Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic Infections
title_full_unstemmed	Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic Infections
title_sort	antibiotic resistance of enterococcus faecalis from persistent endodontic infections
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2020000300448
work_keys_str_mv	AT seguelnatalia antibioticresistanceofenterococcusfaecalisfrompersistentendodonticinfections AT quezadaaguiluzmario antibioticresistanceofenterococcusfaecalisfrompersistentendodonticinfections AT gonzalezrochagerardo antibioticresistanceofenterococcusfaecalisfrompersistentendodonticinfections AT bellotoledohelia antibioticresistanceofenterococcusfaecalisfrompersistentendodonticinfections AT sanchezsanhuezagabriela antibioticresistanceofenterococcusfaecalisfrompersistentendodonticinfections
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Antibiotic Resistance of Enterococcus faecalis from Persistent Endodontic Infections

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