Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin

ABSTRACT: The aim of this in vitro study was to investigate the influence of ethylenediaminetetraacetic acid (EDTA) associated with the benzalkonium chloride (BAK) on the adhesion and formation of Enterococcus faecalis biofilms attached to coated dentin. Discs standard bovine dentin blocks were trea...

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Autores principales:	Titato,Pedro Cesar Gomes, Zancan,Rafaela Fernandes, Pedrinha,Victor Feliz, Andrade,Flaviana Bombarda de, Vivan,Rodrigo Ricci, Duarte,Marco Antonio Hungaro
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2020
Materias:	benzalkonium compounds biofilms chelating agents Enterococcus faecalis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2020000400632
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spelling	oai:scielo:S0718-381X20200004006322020-10-05Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to DentinTitato,Pedro Cesar GomesZancan,Rafaela FernandesPedrinha,Victor FelizAndrade,Flaviana Bombarda deVivan,Rodrigo RicciDuarte,Marco Antonio Hungaro benzalkonium compounds biofilms chelating agents Enterococcus faecalis ABSTRACT: The aim of this in vitro study was to investigate the influence of ethylenediaminetetraacetic acid (EDTA) associated with the benzalkonium chloride (BAK) on the adhesion and formation of Enterococcus faecalis biofilms attached to coated dentin. Discs standard bovine dentin blocks were treated with the coating materials evaluated: Saline solution (control), 17 % EDTA, 17 % EDTA associated with 1 % BAK for 5 minutes and subsequently washed with saline solution. Afterwards, biofilms of E. faecalis (ATCC 29212) were grown on the surface of coated dentin blocks for time intervals of 1 hour and 7 days (n = 20) and were subsequently washed with phosphate-buffered saline (PBS). Bacterial viability and total biovolume were analyzed by confocal laser scanning microscopy (CLSM) using the Live/Dead technique. Nonparametric Kruskal-Wallis followed by Dunn tests were used to determine statistical differences (a = 5 %). The 17 % EDTA + 1 % BAK group showed significantly lower biovolume and bacterial viability values at the end of 1 hour (p < 0.05). After 7 days of contamination, the 17 % EDTA and 17 % EDTA + 1 % BAK groups showed similar results that differed statistically from those of the control group (p < 0.05). The saline solution group showed higher values. The use of BAK associated with EDTA on dentin blocks surfaces before exposure to contamination was able to interfere in the adhesion of E. faecalis to dentin. Also, dentin treatment by BAK associated with a chelating agent influences the secondary biofilm formation, which could have important effects on the long-term success of root canal treatment.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.14 n.4 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2020000400632en10.4067/S0718-381X2020000400632
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	benzalkonium compounds biofilms chelating agents Enterococcus faecalis
spellingShingle	benzalkonium compounds biofilms chelating agents Enterococcus faecalis Titato,Pedro Cesar Gomes Zancan,Rafaela Fernandes Pedrinha,Victor Feliz Andrade,Flaviana Bombarda de Vivan,Rodrigo Ricci Duarte,Marco Antonio Hungaro Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin
description	ABSTRACT: The aim of this in vitro study was to investigate the influence of ethylenediaminetetraacetic acid (EDTA) associated with the benzalkonium chloride (BAK) on the adhesion and formation of Enterococcus faecalis biofilms attached to coated dentin. Discs standard bovine dentin blocks were treated with the coating materials evaluated: Saline solution (control), 17 % EDTA, 17 % EDTA associated with 1 % BAK for 5 minutes and subsequently washed with saline solution. Afterwards, biofilms of E. faecalis (ATCC 29212) were grown on the surface of coated dentin blocks for time intervals of 1 hour and 7 days (n = 20) and were subsequently washed with phosphate-buffered saline (PBS). Bacterial viability and total biovolume were analyzed by confocal laser scanning microscopy (CLSM) using the Live/Dead technique. Nonparametric Kruskal-Wallis followed by Dunn tests were used to determine statistical differences (a = 5 %). The 17 % EDTA + 1 % BAK group showed significantly lower biovolume and bacterial viability values at the end of 1 hour (p < 0.05). After 7 days of contamination, the 17 % EDTA and 17 % EDTA + 1 % BAK groups showed similar results that differed statistically from those of the control group (p < 0.05). The saline solution group showed higher values. The use of BAK associated with EDTA on dentin blocks surfaces before exposure to contamination was able to interfere in the adhesion of E. faecalis to dentin. Also, dentin treatment by BAK associated with a chelating agent influences the secondary biofilm formation, which could have important effects on the long-term success of root canal treatment.
author	Titato,Pedro Cesar Gomes Zancan,Rafaela Fernandes Pedrinha,Victor Feliz Andrade,Flaviana Bombarda de Vivan,Rodrigo Ricci Duarte,Marco Antonio Hungaro
author_facet	Titato,Pedro Cesar Gomes Zancan,Rafaela Fernandes Pedrinha,Victor Feliz Andrade,Flaviana Bombarda de Vivan,Rodrigo Ricci Duarte,Marco Antonio Hungaro
author_sort	Titato,Pedro Cesar Gomes
title	Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin
title_short	Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin
title_full	Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin
title_fullStr	Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin
title_full_unstemmed	Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin
title_sort	influence of edta and its association with benzalkonium chloride on enterococcus faecalis adhesion to dentin
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2020000400632
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Influence of EDTA and its Association with Benzalkonium Chloride on Enterococcus faecalis Adhesion to Dentin

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