Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinus

ABSTRACT: The oral cavity is an ecosystem that provides ideal conditions for the growth of bacteria, the Streptococcus genus is important for the formation of biofilms that lead to the development of dental caries, which affects the population worldwide. The world health organization encourages the...

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Autores principales:	Vaca-Chávez,Maricarmen, Rodríguez-Rojas,Jorge Jesús, Castillo-Velázquez,Uziel, Hernández-Delgadillo,René, Sánchez-Casas,Rosa María, Chávez-Montes,Abelardo, Báez-González,Juan Gabriel, Rodríguez-Rodríguez,José, Martínez-Delgado,Ameyalli Jocelyn, Rodríguez-Luis,Osvelia Esmeralda
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2021
Materias:	biofilms dental plaque Origanum vulgare phytotherapy Streptococcus mutans Streptococcus sobrinus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000100213
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spelling	oai:scielo:S0718-381X20210001002132021-02-16Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinusVaca-Chávez,MaricarmenRodríguez-Rojas,Jorge JesúsCastillo-Velázquez,UzielHernández-Delgadillo,RenéSánchez-Casas,Rosa MaríaChávez-Montes,AbelardoBáez-González,Juan GabrielRodríguez-Rodríguez,JoséMartínez-Delgado,Ameyalli JocelynRodríguez-Luis,Osvelia Esmeralda biofilms dental plaque Origanum vulgare phytotherapy Streptococcus mutans Streptococcus sobrinus ABSTRACT: The oral cavity is an ecosystem that provides ideal conditions for the growth of bacteria, the Streptococcus genus is important for the formation of biofilms that lead to the development of dental caries, which affects the population worldwide. The world health organization encourages the use of plants thanks to its various therapeutic actions. Origanum vulgare L. (oregano), is an aromatic plant with medicinal and culinary properties. The objective of this study was to investigate the in vitro antimicrobial and antibiofilm activity of the ethanolic extract of oregano, against the growth of Streptococcus mutans and Streptococcus sobrinus ATCC. Leaves of the plant were obtained and the ethanolic extract was made by maceration. Antimicrobial activity was evaluated using the Kirby-Bauer method and compared with 2% chlorhexidine, subsequently the extract was incorporated into a hydrogel and its effect on biofilm formation was assessed by fluorescence microscopy and the main compounds were identified. present in the extratco. The study revealed that the extract presented antimicrobial effect against both strains and at 2% it showed high antimicrobial action compared to chlorhexidine at the same concentration, with average inhibition halos of 26.3 mm and 19 mm for each microorganism analyzed, (p < 0.05). Likewise, the hydrogel prepared with 2% extract significantly eliminated the preformed Streptococcus biofilm, at 24 hours of exposure, due to the presence of a variety of chemical groups, such as sterols, triterpenes, flavonoids, flavanones, flavanonol s, lactones. sesquiterpenic, tannins and coumarins. The oregano extract presented high antimicrobial action for both species, with a greater effect towards Streptococcus mutans and an interesting antibiofilm action; These results show the importance of exploring treatment alternatives of plant origin, to be considered as interesting complementary aids in dental therapy.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.15 n.1 20212021-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000100213en10.4067/S0718-381X2021000100213
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	biofilms dental plaque Origanum vulgare phytotherapy Streptococcus mutans Streptococcus sobrinus
spellingShingle	biofilms dental plaque Origanum vulgare phytotherapy Streptococcus mutans Streptococcus sobrinus Vaca-Chávez,Maricarmen Rodríguez-Rojas,Jorge Jesús Castillo-Velázquez,Uziel Hernández-Delgadillo,René Sánchez-Casas,Rosa María Chávez-Montes,Abelardo Báez-González,Juan Gabriel Rodríguez-Rodríguez,José Martínez-Delgado,Ameyalli Jocelyn Rodríguez-Luis,Osvelia Esmeralda Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinus
description	ABSTRACT: The oral cavity is an ecosystem that provides ideal conditions for the growth of bacteria, the Streptococcus genus is important for the formation of biofilms that lead to the development of dental caries, which affects the population worldwide. The world health organization encourages the use of plants thanks to its various therapeutic actions. Origanum vulgare L. (oregano), is an aromatic plant with medicinal and culinary properties. The objective of this study was to investigate the in vitro antimicrobial and antibiofilm activity of the ethanolic extract of oregano, against the growth of Streptococcus mutans and Streptococcus sobrinus ATCC. Leaves of the plant were obtained and the ethanolic extract was made by maceration. Antimicrobial activity was evaluated using the Kirby-Bauer method and compared with 2% chlorhexidine, subsequently the extract was incorporated into a hydrogel and its effect on biofilm formation was assessed by fluorescence microscopy and the main compounds were identified. present in the extratco. The study revealed that the extract presented antimicrobial effect against both strains and at 2% it showed high antimicrobial action compared to chlorhexidine at the same concentration, with average inhibition halos of 26.3 mm and 19 mm for each microorganism analyzed, (p < 0.05). Likewise, the hydrogel prepared with 2% extract significantly eliminated the preformed Streptococcus biofilm, at 24 hours of exposure, due to the presence of a variety of chemical groups, such as sterols, triterpenes, flavonoids, flavanones, flavanonol s, lactones. sesquiterpenic, tannins and coumarins. The oregano extract presented high antimicrobial action for both species, with a greater effect towards Streptococcus mutans and an interesting antibiofilm action; These results show the importance of exploring treatment alternatives of plant origin, to be considered as interesting complementary aids in dental therapy.
author	Vaca-Chávez,Maricarmen Rodríguez-Rojas,Jorge Jesús Castillo-Velázquez,Uziel Hernández-Delgadillo,René Sánchez-Casas,Rosa María Chávez-Montes,Abelardo Báez-González,Juan Gabriel Rodríguez-Rodríguez,José Martínez-Delgado,Ameyalli Jocelyn Rodríguez-Luis,Osvelia Esmeralda
author_facet	Vaca-Chávez,Maricarmen Rodríguez-Rojas,Jorge Jesús Castillo-Velázquez,Uziel Hernández-Delgadillo,René Sánchez-Casas,Rosa María Chávez-Montes,Abelardo Báez-González,Juan Gabriel Rodríguez-Rodríguez,José Martínez-Delgado,Ameyalli Jocelyn Rodríguez-Luis,Osvelia Esmeralda
author_sort	Vaca-Chávez,Maricarmen
title	Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinus
title_short	Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinus
title_full	Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinus
title_fullStr	Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinus
title_full_unstemmed	Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinus
title_sort	antimicrobial and antibiofilm effect of hydrogel with origanum vulgare on culture of streptococcus mutans and streptococcus sobrinus
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000100213
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Antimicrobial and Antibiofilm Effect of Hydrogel with Origanum vulgare on Culture of Streptococcus mutans and Streptococcus sobrinus

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