Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar Ridge

ABSTRACT: The present study investigated the healing response of 12 fresh post-extraction alveolous grafted with particulate autologous teeth to achieve preservation of the post-extraction alveolar ridge. The objective is to elucidate the osteoconductive and osteoinductive properties of the autologo...

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Autores principales:	Moreno-Villagrana,Ana Patricia, Gutiérrez-Valdés,Dulce Haydeé, Flores-Luna,María Guadalupe
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2021
Materias:	teeth graft tooth graft alveolar bone grafting alveolar ridge preservation osteopontin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000300616
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spelling	oai:scielo:S0718-381X20210003006162021-09-21Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar RidgeMoreno-Villagrana,Ana PatriciaGutiérrez-Valdés,Dulce HaydeéFlores-Luna,María Guadalupe teeth graft tooth graft alveolar bone grafting alveolar ridge preservation osteopontin ABSTRACT: The present study investigated the healing response of 12 fresh post-extraction alveolous grafted with particulate autologous teeth to achieve preservation of the post-extraction alveolar ridge. The objective is to elucidate the osteoconductive and osteoinductive properties of the autologous dental graft used as a bone substitute in the alveolar ridge preservation technique. Five patients were included, with at least one hopeless tooth and in need of extraction and preservatio n of the ridge, to receive in the same place a dental implant in prosthetic replacement. In the first surgical stage, dental extractions and preservation of the alveolar ridge were performed, using the teeth extracted and processed with an automatic system as bone substitutes. In the second surgical stage, an incisional bone biopsy was performed in each grafted site, the bone beds were recapitulated in a drilling protocol that allowed the placement of the dental implant, and the harvested bone specimens were prepared for analysis. The histological results of the bone biopsies in all cases showed remnant particles of the dental graft, made up of dentin, partially resorbed, with irregular superficial edges and in close contact with newly forme d bone in transition to mature lamellar bone, in which well differentiated osteocytes were observed. The immunohistochemical results showed a moderate positive expression of osteopontin at the edges of the integrated teeth particles, inside the peritubular dentin space and at the osteodental contact interfaces. In conclusion, the evidence from the study shows that the autologous dental graft is a biocompatible bone substitute, that provides an osteoconductive scaffold that promotes bone cell adhesion and migration for local osteogenesis and that it is associated with moderate in situ expression of osteopontin, which showed a high affinity with mineralized dental tissue, suggesting osteoinductive properties in situ.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.15 n.3 20212021-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000300616en10.4067/S0718-381X2021000300616
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	teeth graft tooth graft alveolar bone grafting alveolar ridge preservation osteopontin
spellingShingle	teeth graft tooth graft alveolar bone grafting alveolar ridge preservation osteopontin Moreno-Villagrana,Ana Patricia Gutiérrez-Valdés,Dulce Haydeé Flores-Luna,María Guadalupe Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar Ridge
description	ABSTRACT: The present study investigated the healing response of 12 fresh post-extraction alveolous grafted with particulate autologous teeth to achieve preservation of the post-extraction alveolar ridge. The objective is to elucidate the osteoconductive and osteoinductive properties of the autologous dental graft used as a bone substitute in the alveolar ridge preservation technique. Five patients were included, with at least one hopeless tooth and in need of extraction and preservatio n of the ridge, to receive in the same place a dental implant in prosthetic replacement. In the first surgical stage, dental extractions and preservation of the alveolar ridge were performed, using the teeth extracted and processed with an automatic system as bone substitutes. In the second surgical stage, an incisional bone biopsy was performed in each grafted site, the bone beds were recapitulated in a drilling protocol that allowed the placement of the dental implant, and the harvested bone specimens were prepared for analysis. The histological results of the bone biopsies in all cases showed remnant particles of the dental graft, made up of dentin, partially resorbed, with irregular superficial edges and in close contact with newly forme d bone in transition to mature lamellar bone, in which well differentiated osteocytes were observed. The immunohistochemical results showed a moderate positive expression of osteopontin at the edges of the integrated teeth particles, inside the peritubular dentin space and at the osteodental contact interfaces. In conclusion, the evidence from the study shows that the autologous dental graft is a biocompatible bone substitute, that provides an osteoconductive scaffold that promotes bone cell adhesion and migration for local osteogenesis and that it is associated with moderate in situ expression of osteopontin, which showed a high affinity with mineralized dental tissue, suggesting osteoinductive properties in situ.
author	Moreno-Villagrana,Ana Patricia Gutiérrez-Valdés,Dulce Haydeé Flores-Luna,María Guadalupe
author_facet	Moreno-Villagrana,Ana Patricia Gutiérrez-Valdés,Dulce Haydeé Flores-Luna,María Guadalupe
author_sort	Moreno-Villagrana,Ana Patricia
title	Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar Ridge
title_short	Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar Ridge
title_full	Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar Ridge
title_fullStr	Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar Ridge
title_full_unstemmed	Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar Ridge
title_sort	immunohistochemical analysis of alveolar bone preserved with autologous teeth graft. osteopontin expression and its regulatory functions in preserved alveolar ridge
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000300616
work_keys_str_mv	AT morenovillagranaanapatricia immunohistochemicalanalysisofalveolarbonepreservedwithautologousteethgraftosteopontinexpressionanditsregulatoryfunctionsinpreservedalveolarridge AT gutierrezvaldesdulcehaydee immunohistochemicalanalysisofalveolarbonepreservedwithautologousteethgraftosteopontinexpressionanditsregulatoryfunctionsinpreservedalveolarridge AT floreslunamariaguadalupe immunohistochemicalanalysisofalveolarbonepreservedwithautologousteethgraftosteopontinexpressionanditsregulatoryfunctionsinpreservedalveolarridge
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Immunohistochemical Analysis of Alveolar Bone Preserved with Autologous Teeth Graft. Osteopontin Expression and its Regulatory Functions in Preserved Alveolar Ridge

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