Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutans

ABSTRACT: Antibacterial activity of zinc oxide nanoparticles in self-curing acrylic resin against Streptococcus mutans. The main objective of this study was to investigate whether nanoparticles of zinc oxide (ZnO) in self-curing acrylic resin, hav e antimicrobial properties against Streptococcus mut...

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Autores principales:	Quezada-Morales,Pía, Luengo-Machuca,Luis, Quezada-Aguiluz,Mario, Melendrez-Castro,Manuel, Bello-Toledo,Helia, González-Rocha,Gerardo, Sánchez-Sanhueza,Gabriela
Lenguaje:	English
Publicado:	Universidad de La Frontera. Facultad de Medicina 2021
Materias:	Biofilm Minimum Inhibitory Concentration (MIC) Nanoparticles Streptococcus mutans Zinc oxide
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000300694
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spelling	oai:scielo:S0718-381X20210003006942021-09-21Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutansQuezada-Morales,PíaLuengo-Machuca,LuisQuezada-Aguiluz,MarioMelendrez-Castro,ManuelBello-Toledo,HeliaGonzález-Rocha,GerardoSánchez-Sanhueza,Gabriela Biofilm Minimum Inhibitory Concentration (MIC) Nanoparticles Streptococcus mutans Zinc oxide ABSTRACT: Antibacterial activity of zinc oxide nanoparticles in self-curing acrylic resin against Streptococcus mutans. The main objective of this study was to investigate whether nanoparticles of zinc oxide (ZnO) in self-curing acrylic resin, hav e antimicrobial properties against Streptococcus mutans, one of the microorganisms involved in the development of caries. Self- cured acrylic resin samples were prepared by incorporating ZnO nanoparticles at different concentrations based on the minimum inhibitory concentration (MIC) for Streptococcus mutans ATCC 25175. Antibacterial activity against a biofilm was evaluated in samples that were aged in artificial saliva for different times using spectral confocal laser microscopy and scanning electron microscopy. Kruskal-Wallis test using IBM SPSS Statistics version 23.0 software (SPSS Inc. ®, Chicago, IL, United States) were used, establishing the value of p <0.05 for statistical significance. The volume of the total biomass that formed in the samples aged for one day was significantly lower than the volume of the total biomass that was formed in those aged for additional days (p <0.001). Electron microscopy analysis revealed high porosity surfaces in all samples. Bacterial clusters wer e located next to large pores and irregular surfaces, while smooth surfaces had defined and linear organization cocci or simple chains. Considering the limitations of this study, the results suggest that the antibacterial activity of ZnO nanoparticles add ed to self-curing acrylic (ALIKE) is effective, mainly in fresh 1-day samples, independent of their concentration, and in samples with 16 MIC aged for 14 days, indicating it does not lose its antibacterial activity despite setting for more days. In addition, the ZnO nanoparticles added to ALIKE have the ability to inhibit the formation of biofilms, although they do not minimize the number of viable bacteria.info:eu-repo/semantics/openAccessUniversidad de La Frontera. Facultad de MedicinaInternational journal of odontostomatology v.15 n.3 20212021-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000300694en10.4067/S0718-381X2021000300694
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Biofilm Minimum Inhibitory Concentration (MIC) Nanoparticles Streptococcus mutans Zinc oxide
spellingShingle	Biofilm Minimum Inhibitory Concentration (MIC) Nanoparticles Streptococcus mutans Zinc oxide Quezada-Morales,Pía Luengo-Machuca,Luis Quezada-Aguiluz,Mario Melendrez-Castro,Manuel Bello-Toledo,Helia González-Rocha,Gerardo Sánchez-Sanhueza,Gabriela Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutans
description	ABSTRACT: Antibacterial activity of zinc oxide nanoparticles in self-curing acrylic resin against Streptococcus mutans. The main objective of this study was to investigate whether nanoparticles of zinc oxide (ZnO) in self-curing acrylic resin, hav e antimicrobial properties against Streptococcus mutans, one of the microorganisms involved in the development of caries. Self- cured acrylic resin samples were prepared by incorporating ZnO nanoparticles at different concentrations based on the minimum inhibitory concentration (MIC) for Streptococcus mutans ATCC 25175. Antibacterial activity against a biofilm was evaluated in samples that were aged in artificial saliva for different times using spectral confocal laser microscopy and scanning electron microscopy. Kruskal-Wallis test using IBM SPSS Statistics version 23.0 software (SPSS Inc. ®, Chicago, IL, United States) were used, establishing the value of p <0.05 for statistical significance. The volume of the total biomass that formed in the samples aged for one day was significantly lower than the volume of the total biomass that was formed in those aged for additional days (p <0.001). Electron microscopy analysis revealed high porosity surfaces in all samples. Bacterial clusters wer e located next to large pores and irregular surfaces, while smooth surfaces had defined and linear organization cocci or simple chains. Considering the limitations of this study, the results suggest that the antibacterial activity of ZnO nanoparticles add ed to self-curing acrylic (ALIKE) is effective, mainly in fresh 1-day samples, independent of their concentration, and in samples with 16 MIC aged for 14 days, indicating it does not lose its antibacterial activity despite setting for more days. In addition, the ZnO nanoparticles added to ALIKE have the ability to inhibit the formation of biofilms, although they do not minimize the number of viable bacteria.
author	Quezada-Morales,Pía Luengo-Machuca,Luis Quezada-Aguiluz,Mario Melendrez-Castro,Manuel Bello-Toledo,Helia González-Rocha,Gerardo Sánchez-Sanhueza,Gabriela
author_facet	Quezada-Morales,Pía Luengo-Machuca,Luis Quezada-Aguiluz,Mario Melendrez-Castro,Manuel Bello-Toledo,Helia González-Rocha,Gerardo Sánchez-Sanhueza,Gabriela
author_sort	Quezada-Morales,Pía
title	Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutans
title_short	Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutans
title_full	Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutans
title_fullStr	Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutans
title_full_unstemmed	Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutans
title_sort	antibacterial activity of zinc oxide nanoparticles in self- curing acrylic resin against streptococcus mutans
publisher	Universidad de La Frontera. Facultad de Medicina
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-381X2021000300694
work_keys_str_mv	AT quezadamoralespia antibacterialactivityofzincoxidenanoparticlesinselfcuringacrylicresinagainststreptococcusmutans AT luengomachucaluis antibacterialactivityofzincoxidenanoparticlesinselfcuringacrylicresinagainststreptococcusmutans AT quezadaaguiluzmario antibacterialactivityofzincoxidenanoparticlesinselfcuringacrylicresinagainststreptococcusmutans AT melendrezcastromanuel antibacterialactivityofzincoxidenanoparticlesinselfcuringacrylicresinagainststreptococcusmutans AT bellotoledohelia antibacterialactivityofzincoxidenanoparticlesinselfcuringacrylicresinagainststreptococcusmutans AT gonzalezrochagerardo antibacterialactivityofzincoxidenanoparticlesinselfcuringacrylicresinagainststreptococcusmutans AT sanchezsanhuezagabriela antibacterialactivityofzincoxidenanoparticlesinselfcuringacrylicresinagainststreptococcusmutans
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Antibacterial Activity of Zinc Oxide Nanoparticles in Self- Curing Acrylic Resin Against Streptococcus mutans

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