Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)

The nuclear DNA content (2C value) was determined in the commercial fish Galaxias maculatus (Galaxiidae) was determined by microdensitometry of erythrocyte nuclei after Feulgen staining; rainbow trout erythrocytes with a known 2C value were used as a standard. The 2C value of G. maculatus was 2.21 ±...

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Autores principales:	Jara-Seguel,Pedro, Valdebenito,Iván, Palma-Rojas,Claudio, Rebolledo,Christian
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2008
Materias:	Galaxias maculatus nuclear DNA content nucleotypic effect Galaxiidae Osmeriformes Chile
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2008000100007
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id	oai:scielo:S0718-560X2008000100007
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spelling	oai:scielo:S0718-560X20080001000072009-02-03Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)Jara-Seguel,PedroValdebenito,IvánPalma-Rojas,ClaudioRebolledo,Christian Galaxias maculatus nuclear DNA content nucleotypic effect Galaxiidae Osmeriformes Chile The nuclear DNA content (2C value) was determined in the commercial fish Galaxias maculatus (Galaxiidae) was determined by microdensitometry of erythrocyte nuclei after Feulgen staining; rainbow trout erythrocytes with a known 2C value were used as a standard. The 2C value of G. maculatus was 2.21 ± 0.12 pg and its C value was equivalent to 1.105 pg (1,082.9 Mbp). This C value is within the range recorded for other osmeriform species (0.62-3.2 pg). The average sperm head diameter of G. maculatus is lower than the average sperm head diameter of rainbow trout (used as a standard), which coincides with the differences observed in the nuclear DNA content of both species. This information increases the genome data available for G. maculatus and might be useful in future programs dealing with its genetic manipulation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.36 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2008000100007en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Galaxias maculatus nuclear DNA content nucleotypic effect Galaxiidae Osmeriformes Chile
spellingShingle	Galaxias maculatus nuclear DNA content nucleotypic effect Galaxiidae Osmeriformes Chile Jara-Seguel,Pedro Valdebenito,Iván Palma-Rojas,Claudio Rebolledo,Christian Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
description	The nuclear DNA content (2C value) was determined in the commercial fish Galaxias maculatus (Galaxiidae) was determined by microdensitometry of erythrocyte nuclei after Feulgen staining; rainbow trout erythrocytes with a known 2C value were used as a standard. The 2C value of G. maculatus was 2.21 ± 0.12 pg and its C value was equivalent to 1.105 pg (1,082.9 Mbp). This C value is within the range recorded for other osmeriform species (0.62-3.2 pg). The average sperm head diameter of G. maculatus is lower than the average sperm head diameter of rainbow trout (used as a standard), which coincides with the differences observed in the nuclear DNA content of both species. This information increases the genome data available for G. maculatus and might be useful in future programs dealing with its genetic manipulation.
author	Jara-Seguel,Pedro Valdebenito,Iván Palma-Rojas,Claudio Rebolledo,Christian
author_facet	Jara-Seguel,Pedro Valdebenito,Iván Palma-Rojas,Claudio Rebolledo,Christian
author_sort	Jara-Seguel,Pedro
title	Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
title_short	Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
title_full	Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
title_fullStr	Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
title_full_unstemmed	Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)
title_sort	nuclear dna content in galaxias maculatus (teleostei: osmeriformes: galaxiidae)
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2008000100007
work_keys_str_mv	AT jaraseguelpedro nucleardnacontentingalaxiasmaculatusteleosteiosmeriformesgalaxiidae AT valdebenitoivan nucleardnacontentingalaxiasmaculatusteleosteiosmeriformesgalaxiidae AT palmarojasclaudio nucleardnacontentingalaxiasmaculatusteleosteiosmeriformesgalaxiidae AT rebolledochristian nucleardnacontentingalaxiasmaculatusteleosteiosmeriformesgalaxiidae
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Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae)

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