Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods

We evaluated the effect of three variables (cryoprotectant temperature, light regime, and time of exposure to the cryoprotectant) throughout the equilibrium period during cryopreservation on the viability of the microalga Chaetoceros calcitrans (Paulsen). For this, the cryoprotectant dimethyl sulfox...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Salas-Leiva,Joan Sebastián, Dupré,Enrique
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2011
Materias:	cryopreservation diatoms DMSO temperature light equilibrium period
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000200008
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-560X2011000200008
record_format	dspace
spelling	oai:scielo:S0718-560X20110002000082011-07-26Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periodsSalas-Leiva,Joan SebastiánDupré,Enrique cryopreservation diatoms DMSO temperature light equilibrium period We evaluated the effect of three variables (cryoprotectant temperature, light regime, and time of exposure to the cryoprotectant) throughout the equilibrium period during cryopreservation on the viability of the microalga Chaetoceros calcitrans (Paulsen). For this, the cryoprotectant dimethyl sulfoxide (DMSO) at 5% (v/v) was added at three different temperatures (4, 10, and 25°C) before placing the microalgae in cryobiological straws for freezing. Once inside the cryobiological straws, the microalgal-cryoprotectant suspensions were subjected to the following light regimes for 15 or 45 min: complete light, complete darkness, light/darkness, and darkness/light. Suspensions were then frozen under controlled conditions and stored in liquid nitrogen. The viability index proposed by Cañavate & Lubian (1995b) was used to measure microalgal viability after cryopreservation. Results indicated that it is necessary to use a cryoprotectant to ensure the viable cryopreservation of C. calcitrans. Statistical analyses showed that the temperature of the DMSO influenced the viability of cryopreserved microalgae and that there was no synergistic effect between the other variables studied. The viabilities obtained with DMSO at 25°C, 10°C, and 4°C were 34.9%, 27.8%, and 20.6%, respectively.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.39 n.2 20112011-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000200008en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cryopreservation diatoms DMSO temperature light equilibrium period
spellingShingle	cryopreservation diatoms DMSO temperature light equilibrium period Salas-Leiva,Joan Sebastián Dupré,Enrique Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods
description	We evaluated the effect of three variables (cryoprotectant temperature, light regime, and time of exposure to the cryoprotectant) throughout the equilibrium period during cryopreservation on the viability of the microalga Chaetoceros calcitrans (Paulsen). For this, the cryoprotectant dimethyl sulfoxide (DMSO) at 5% (v/v) was added at three different temperatures (4, 10, and 25°C) before placing the microalgae in cryobiological straws for freezing. Once inside the cryobiological straws, the microalgal-cryoprotectant suspensions were subjected to the following light regimes for 15 or 45 min: complete light, complete darkness, light/darkness, and darkness/light. Suspensions were then frozen under controlled conditions and stored in liquid nitrogen. The viability index proposed by Cañavate & Lubian (1995b) was used to measure microalgal viability after cryopreservation. Results indicated that it is necessary to use a cryoprotectant to ensure the viable cryopreservation of C. calcitrans. Statistical analyses showed that the temperature of the DMSO influenced the viability of cryopreserved microalgae and that there was no synergistic effect between the other variables studied. The viabilities obtained with DMSO at 25°C, 10°C, and 4°C were 34.9%, 27.8%, and 20.6%, respectively.
author	Salas-Leiva,Joan Sebastián Dupré,Enrique
author_facet	Salas-Leiva,Joan Sebastián Dupré,Enrique
author_sort	Salas-Leiva,Joan Sebastián
title	Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods
title_short	Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods
title_full	Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods
title_fullStr	Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods
title_full_unstemmed	Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods
title_sort	cryopreservation of the microalgae chaetoceros calcitrans (paulsen): analysis of the effect of dmso temperature and light regime during different equilibrium periods
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000200008
work_keys_str_mv	AT salasleivajoansebastian cryopreservationofthemicroalgaechaetoceroscalcitranspaulsenanalysisoftheeffectofdmsotemperatureandlightregimeduringdifferentequilibriumperiods AT dupreenrique cryopreservationofthemicroalgaechaetoceroscalcitranspaulsenanalysisoftheeffectofdmsotemperatureandlightregimeduringdifferentequilibriumperiods
_version_	1714205080084807680

Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods

Ejemplares similares