Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)

In January 1999, unialgal cultures of the diatom Thalassiosira sp., solate from natural phyto-plankton assemblages from Potter Cove, Antarctica, were exposed to solar ultraviolet radiation (UVR, 280400 nm) in order to study the long-term acclimation of this species. Ultraviolet radiation B (UVBR, 28...

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Autores principales:	Hernando,Marcelo, Malanga,Gabriela, Puntarulo,Susana, Ferreyra,Gustavo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2011
Materias:	β-carotene α-tocopherol growth rate diatoms MAAs photoprotection Antarctica
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000300001
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spelling	oai:scielo:S0718-560X20110003000012014-11-05Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)Hernando,MarceloMalanga,GabrielaPuntarulo,SusanaFerreyra,Gustavo β-carotene α-tocopherol growth rate diatoms MAAs photoprotection Antarctica In January 1999, unialgal cultures of the diatom Thalassiosira sp., solate from natural phyto-plankton assemblages from Potter Cove, Antarctica, were exposed to solar ultraviolet radiation (UVR, 280400 nm) in order to study the long-term acclimation of this species. Ultraviolet radiation B (UVBR, 280-315 nm) inhibited the growth rate during the first and second days of exposure. No UVBR inhibition was observed on the third day. The initial content of α-tocopherol (13 pmol (10(4) cell)-1) showed a marked decrease during the exponential growth phase (4 pmol (10(4) cell-1) by day 3). The initial content of β-carotene (3 pmol (10(4) cell)-1) did not show significant differences over time in cells exposed to UVBR. Two mycosporine-like amino acids (MAAs) were identified: porphyra-334 and shinorine. Cellular concentrations of MAAs increased significantly on days 2 and 3, and exposure of the algae to UVBR significantly enhanced this value. The relative importance of MAAs concentration was significant (P < 0.05) in relation to the α-tocopherol content. A positive correlation was shown between cellular MAAs concentration and growth rate. Our results suggest that photoprotection against UV-induced damage is characterized by short-term consumption of α-tocopherol and longer-term synthesis of MAAs. The UVBR damage/repair ratio during long-term exposure involves the combined action of several endogenous factors within the cell, with MAAs synthesis being the most effective factor related to photoprotection.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.39 n.3 20112011-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000300001en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	β-carotene α-tocopherol growth rate diatoms MAAs photoprotection Antarctica
spellingShingle	β-carotene α-tocopherol growth rate diatoms MAAs photoprotection Antarctica Hernando,Marcelo Malanga,Gabriela Puntarulo,Susana Ferreyra,Gustavo Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)
description	In January 1999, unialgal cultures of the diatom Thalassiosira sp., solate from natural phyto-plankton assemblages from Potter Cove, Antarctica, were exposed to solar ultraviolet radiation (UVR, 280400 nm) in order to study the long-term acclimation of this species. Ultraviolet radiation B (UVBR, 280-315 nm) inhibited the growth rate during the first and second days of exposure. No UVBR inhibition was observed on the third day. The initial content of α-tocopherol (13 pmol (10(4) cell)-1) showed a marked decrease during the exponential growth phase (4 pmol (10(4) cell-1) by day 3). The initial content of β-carotene (3 pmol (10(4) cell)-1) did not show significant differences over time in cells exposed to UVBR. Two mycosporine-like amino acids (MAAs) were identified: porphyra-334 and shinorine. Cellular concentrations of MAAs increased significantly on days 2 and 3, and exposure of the algae to UVBR significantly enhanced this value. The relative importance of MAAs concentration was significant (P < 0.05) in relation to the α-tocopherol content. A positive correlation was shown between cellular MAAs concentration and growth rate. Our results suggest that photoprotection against UV-induced damage is characterized by short-term consumption of α-tocopherol and longer-term synthesis of MAAs. The UVBR damage/repair ratio during long-term exposure involves the combined action of several endogenous factors within the cell, with MAAs synthesis being the most effective factor related to photoprotection.
author	Hernando,Marcelo Malanga,Gabriela Puntarulo,Susana Ferreyra,Gustavo
author_facet	Hernando,Marcelo Malanga,Gabriela Puntarulo,Susana Ferreyra,Gustavo
author_sort	Hernando,Marcelo
title	Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)
title_short	Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)
title_full	Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)
title_fullStr	Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)
title_full_unstemmed	Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)
title_sort	fotoprotección antioxidante no-enzimática contra el potencial daño inducido por uvbr en una diatomea antártica (thalassiosira sp.)
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000300001
work_keys_str_mv	AT hernandomarcelo fotoproteccionantioxidantenoenzimaticacontraelpotencialdanoinducidoporuvbrenunadiatomeaantarticathalassiosirasp AT malangagabriela fotoproteccionantioxidantenoenzimaticacontraelpotencialdanoinducidoporuvbrenunadiatomeaantarticathalassiosirasp AT puntarulosusana fotoproteccionantioxidantenoenzimaticacontraelpotencialdanoinducidoporuvbrenunadiatomeaantarticathalassiosirasp AT ferreyragustavo fotoproteccionantioxidantenoenzimaticacontraelpotencialdanoinducidoporuvbrenunadiatomeaantarticathalassiosirasp
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Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.)

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