Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target

Infectious pancreatic necrosis virus (IPNV) is the causal agent of a highly prevalent disease that affects salmonid fish, mostly during their fresh water life period. Like many other viruses, IPNV produces highly heterogeneous populations. Therefore, diagnostic methods need to be checked constantly...

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Autores principales:	Eissler,Yoanna, Pavlov,María Soledad, Conejeros,Pablo, Espinoza,Juan Carlos, Kuznar,Juan
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2011
Materias:	infectious pancreatic necrosis virus (IPNV) real-time RT-PCR assay VP1 gene phylogenetic tree
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000300014
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spelling	oai:scielo:S0718-560X20110003000142014-11-05Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a targetEissler,YoannaPavlov,María SoledadConejeros,PabloEspinoza,Juan CarlosKuznar,Juan infectious pancreatic necrosis virus (IPNV) real-time RT-PCR assay VP1 gene phylogenetic tree Infectious pancreatic necrosis virus (IPNV) is the causal agent of a highly prevalent disease that affects salmonid fish, mostly during their fresh water life period. Like many other viruses, IPNV produces highly heterogeneous populations. Therefore, diagnostic methods need to be checked constantly so that no variants of the virus escape detection. The IPNV genome is composed of two double-stranded RNA segments: A and B, polymerase chain reaction (PCR) methods normally use segment A as a target. In order to develop an optimized protocol to diagnose IPNV, we present a real-time RT-PCR (reverse transcription) technique, using primers designed to recognize segment B of the virus. To validate the ubiquity of the primers used, the IPNV isolates tested were sequenced and compared with previously published cladograms, which include a wide spectrum of genogroups. These primers made it possible to detect viral isolates belonging to genogroups 1 and 5, which were obtained from different locations linked to fish farming. As expected, we were able to detect the virus from distant Aquabirnavirus genogroups.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.39 n.3 20112011-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000300014en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	infectious pancreatic necrosis virus (IPNV) real-time RT-PCR assay VP1 gene phylogenetic tree
spellingShingle	infectious pancreatic necrosis virus (IPNV) real-time RT-PCR assay VP1 gene phylogenetic tree Eissler,Yoanna Pavlov,María Soledad Conejeros,Pablo Espinoza,Juan Carlos Kuznar,Juan Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target
description	Infectious pancreatic necrosis virus (IPNV) is the causal agent of a highly prevalent disease that affects salmonid fish, mostly during their fresh water life period. Like many other viruses, IPNV produces highly heterogeneous populations. Therefore, diagnostic methods need to be checked constantly so that no variants of the virus escape detection. The IPNV genome is composed of two double-stranded RNA segments: A and B, polymerase chain reaction (PCR) methods normally use segment A as a target. In order to develop an optimized protocol to diagnose IPNV, we present a real-time RT-PCR (reverse transcription) technique, using primers designed to recognize segment B of the virus. To validate the ubiquity of the primers used, the IPNV isolates tested were sequenced and compared with previously published cladograms, which include a wide spectrum of genogroups. These primers made it possible to detect viral isolates belonging to genogroups 1 and 5, which were obtained from different locations linked to fish farming. As expected, we were able to detect the virus from distant Aquabirnavirus genogroups.
author	Eissler,Yoanna Pavlov,María Soledad Conejeros,Pablo Espinoza,Juan Carlos Kuznar,Juan
author_facet	Eissler,Yoanna Pavlov,María Soledad Conejeros,Pablo Espinoza,Juan Carlos Kuznar,Juan
author_sort	Eissler,Yoanna
title	Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target
title_short	Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target
title_full	Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target
title_fullStr	Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target
title_full_unstemmed	Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target
title_sort	detection and quantification of chilean strains of infectious pancreatic necrosis virus by real-time rt-pcr assays using segment b as a target
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2011000300014
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Detection and quantification of Chilean strains of infectious pancreatic necrosis virus by real-time RT-PCR assays using segment B as a target

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