Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837)
During first gamete interaction, oligosaccharides on the glycoproteins present in oocyte envelopes of the rock shrimp, Rhynchocinetes typus, play an important role in spermatozoon recognition prior to the onset of penetration. These oligosaccharides have terminal monosaccharides that may be recogniz...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
2012
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oai:scielo:S0718-560X20120001000032012-05-16Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837)Dupré,EnriqueGómez,DanielAraya,AndreaGallardo,Cristian gametes oocyte recognition sperm receptors lectins vitelline coat chorion During first gamete interaction, oligosaccharides on the glycoproteins present in oocyte envelopes of the rock shrimp, Rhynchocinetes typus, play an important role in spermatozoon recognition prior to the onset of penetration. These oligosaccharides have terminal monosaccharides that may be recognized by means of specific lectins. A variety of marine species have monosaccharides on their oocyte envelopes, allowing them to interact with the spermatozoa. The number and location of the monosaccharides varies during oocyte maturation. However, this phenomenon has not been studied in shrimp. The present study evaluates the presence and location of monosaccharides on oocyte envelopes during maturation using seven specific lectins conjugated to FITC (GNL, LCA, Con-A BS-1, LTA, GLS-II,DBA); these recognize mannose (α 1-3) mannose, α-mannose, α-glucose, glucose, galactose, fucose, N-acetyl-glucosamine, and N-acetyl-galactosamine, respectively. Participation of these carbohydrates in the fertilization process was determined through the insemination of fresh oocytes with spermatozoa previously incubated with the different monosaccharides that block the active sites of the spermatozoa, inhibiting fertilization. The results showed the presence of N-acetyl-glucosamine and mannose on the oocyte envelopes, indicating that these sugars could be the receptors during the first gamete interaction. The results also suggested that glucose could be a complementary receptor to N-acetyl-glucosamine and mannose since, despite the low concentration of glucose on the envelope, this sugar generated a high degree of fertilization inhibition.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.40 n.1 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2012000100003en |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
gametes oocyte recognition sperm receptors lectins vitelline coat chorion |
| spellingShingle |
gametes oocyte recognition sperm receptors lectins vitelline coat chorion Dupré,Enrique Gómez,Daniel Araya,Andrea Gallardo,Cristian Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837) |
| description |
During first gamete interaction, oligosaccharides on the glycoproteins present in oocyte envelopes of the rock shrimp, Rhynchocinetes typus, play an important role in spermatozoon recognition prior to the onset of penetration. These oligosaccharides have terminal monosaccharides that may be recognized by means of specific lectins. A variety of marine species have monosaccharides on their oocyte envelopes, allowing them to interact with the spermatozoa. The number and location of the monosaccharides varies during oocyte maturation. However, this phenomenon has not been studied in shrimp. The present study evaluates the presence and location of monosaccharides on oocyte envelopes during maturation using seven specific lectins conjugated to FITC (GNL, LCA, Con-A BS-1, LTA, GLS-II,DBA); these recognize mannose (α 1-3) mannose, α-mannose, α-glucose, glucose, galactose, fucose, N-acetyl-glucosamine, and N-acetyl-galactosamine, respectively. Participation of these carbohydrates in the fertilization process was determined through the insemination of fresh oocytes with spermatozoa previously incubated with the different monosaccharides that block the active sites of the spermatozoa, inhibiting fertilization. The results showed the presence of N-acetyl-glucosamine and mannose on the oocyte envelopes, indicating that these sugars could be the receptors during the first gamete interaction. The results also suggested that glucose could be a complementary receptor to N-acetyl-glucosamine and mannose since, despite the low concentration of glucose on the envelope, this sugar generated a high degree of fertilization inhibition. |
| author |
Dupré,Enrique Gómez,Daniel Araya,Andrea Gallardo,Cristian |
| author_facet |
Dupré,Enrique Gómez,Daniel Araya,Andrea Gallardo,Cristian |
| author_sort |
Dupré,Enrique |
| title |
Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837) |
| title_short |
Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837) |
| title_full |
Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837) |
| title_fullStr |
Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837) |
| title_full_unstemmed |
Role of egg surface glycoconjugate in the fertilization of the rock shrimp Rhynchocinetes typus (Milne-Edwards, 1837) |
| title_sort |
role of egg surface glycoconjugate in the fertilization of the rock shrimp rhynchocinetes typus (milne-edwards, 1837) |
| publisher |
Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar |
| publishDate |
2012 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2012000100003 |
| work_keys_str_mv |
AT dupreenrique roleofeggsurfaceglycoconjugateinthefertilizationoftherockshrimprhynchocinetestypusmilneedwards1837 AT gomezdaniel roleofeggsurfaceglycoconjugateinthefertilizationoftherockshrimprhynchocinetestypusmilneedwards1837 AT arayaandrea roleofeggsurfaceglycoconjugateinthefertilizationoftherockshrimprhynchocinetestypusmilneedwards1837 AT gallardocristian roleofeggsurfaceglycoconjugateinthefertilizationoftherockshrimprhynchocinetestypusmilneedwards1837 |
| _version_ |
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