An evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers

In trout farming males exhibits lower growth rate and precocious sexual maturation in contrast to females. Since these traits are detrimental for intensive production, all females populations are most appreciated by fish farmers. Molecular markers sex-specific have been developed for rainbow trout t...

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Autores principales: López,María E, Araneda,Cristian
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2012
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2012000400024
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spelling oai:scielo:S0718-560X20120004000242013-04-12An evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markersLópez,María EAraneda,Cristian Oncorhynchus mykiss OmyP9 Omy163 sex marker diagnostic test In trout farming males exhibits lower growth rate and precocious sexual maturation in contrast to females. Since these traits are detrimental for intensive production, all females populations are most appreciated by fish farmers. Molecular markers sex-specific have been developed for rainbow trout that can be useful to diagnose the phenotypic sex of individuals. We evaluated the use of two SCAR markers (OmyP9 & Omy163), which show polymorphisms between males and females in rainbow trout as a diagnostic test for sexing farmed trout. Adult trout (n = 131) were genotyped to assess the association of the SCAR markers with phenotypic sex. To evaluate the correct performance of the SCAR marker in sex diagnosis, each marker and both were analyzed to estimate its specificity (the proportion of males that are correctly identified), sensitivity (the proportion of females that are correctly identified) and predictive value (the probability of the correct positive or negative female identification). Significant associations with phenotypic sex of both SCAR markers with sex were found. The sensitivity and predictive (positive and negative) indexes show higher values when both SCAR markers were considered (95.7, 77.4 and 94.4%, respectively). For joined SCAR markers a likelihood ratio (LR+) of 3.43 was obtained indicating its utility to establish a diagnostic test for sexing trout by use of marker-based analysis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.40 n.4 20122012-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2012000400024en103856/vol40-issue4-fulltext-24
institution Scielo Chile
collection Scielo Chile
language English
topic Oncorhynchus mykiss
OmyP9
Omy163
sex marker
diagnostic test
spellingShingle Oncorhynchus mykiss
OmyP9
Omy163
sex marker
diagnostic test
López,María E
Araneda,Cristian
An evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers
description In trout farming males exhibits lower growth rate and precocious sexual maturation in contrast to females. Since these traits are detrimental for intensive production, all females populations are most appreciated by fish farmers. Molecular markers sex-specific have been developed for rainbow trout that can be useful to diagnose the phenotypic sex of individuals. We evaluated the use of two SCAR markers (OmyP9 & Omy163), which show polymorphisms between males and females in rainbow trout as a diagnostic test for sexing farmed trout. Adult trout (n = 131) were genotyped to assess the association of the SCAR markers with phenotypic sex. To evaluate the correct performance of the SCAR marker in sex diagnosis, each marker and both were analyzed to estimate its specificity (the proportion of males that are correctly identified), sensitivity (the proportion of females that are correctly identified) and predictive value (the probability of the correct positive or negative female identification). Significant associations with phenotypic sex of both SCAR markers with sex were found. The sensitivity and predictive (positive and negative) indexes show higher values when both SCAR markers were considered (95.7, 77.4 and 94.4%, respectively). For joined SCAR markers a likelihood ratio (LR+) of 3.43 was obtained indicating its utility to establish a diagnostic test for sexing trout by use of marker-based analysis.
author López,María E
Araneda,Cristian
author_facet López,María E
Araneda,Cristian
author_sort López,María E
title An evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers
title_short An evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers
title_full An evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers
title_fullStr An evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers
title_full_unstemmed An evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers
title_sort evaluation of a diagnostic test to identify the sex of farmed rainbow trout, using sex-specific molecular markers
publisher Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate 2012
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2012000400024
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