Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin Lytechinus

Marine organisms are rich sources of natural products that, among other activities, help to maintain the species equilibrium. Samples of the marine brown alga Canistrocarpus cervicornis (Kützing) De Paula & De Clerck were collected in Búzios, Rio de Janeiro, Brazil, in June 2006. The extract...

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Autores principales:	Ortiz-Ramírez,Fredy A, Aparecida-Vallim,Magui, Negrão-Cavalcanti,Diana, Laneuville-Teixeira,Valéria
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2013
Materias:	natural products diterpenes brown seaweed dolastanes Lytechinus variegatus Brazil
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000200008
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spelling	oai:scielo:S0718-560X20130002000082013-08-13Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin LytechinusOrtiz-Ramírez,Fredy AAparecida-Vallim,MaguiNegrão-Cavalcanti,DianaLaneuville-Teixeira,Valéria natural products diterpenes brown seaweed dolastanes Lytechinus variegatus Brazil Marine organisms are rich sources of natural products that, among other activities, help to maintain the species equilibrium. Samples of the marine brown alga Canistrocarpus cervicornis (Kützing) De Paula & De Clerck were collected in Búzios, Rio de Janeiro, Brazil, in June 2006. The extract was obtained in CH2Cl2 and subjected to fractionation by chromatographic methods in order to isolate and purify the compound (4R, 7R, 14S)-4α, 7α-diacethoxy-14-hydroxydolastane-1(15), 8-dien. Then, the effects of the extract and the dolastane diterpene on zygotes and gametes of the sea urchin Lytechinus variegatus were evaluated. The exposure of male and female gametes to the C. cervicornis extract promoted, respectively, a reduction of 10-30% in fertilization and a 20 to 70% decrease in the number of eggs. Furthermore, the exposure of zygotes to the extract inhibited their development up to 86.7 ± 1.6% (at a concentration of 250 µg mL-1), as well as generating abnormalities in 39-50% of zygotes. The results of the dolastane diterpene showed no evidence of inhibition in the zygotes' development, thought it was proved to induce anomalies. At higher concentrations (25 and 50 µg mL-1), it was possible to observe cell lyses.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.41 n.2 20132013-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000200008en10.3856/vol41-issue2-fulltext-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	natural products diterpenes brown seaweed dolastanes Lytechinus variegatus Brazil
spellingShingle	natural products diterpenes brown seaweed dolastanes Lytechinus variegatus Brazil Ortiz-Ramírez,Fredy A Aparecida-Vallim,Magui Negrão-Cavalcanti,Diana Laneuville-Teixeira,Valéria Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin Lytechinus
description	Marine organisms are rich sources of natural products that, among other activities, help to maintain the species equilibrium. Samples of the marine brown alga Canistrocarpus cervicornis (Kützing) De Paula & De Clerck were collected in Búzios, Rio de Janeiro, Brazil, in June 2006. The extract was obtained in CH2Cl2 and subjected to fractionation by chromatographic methods in order to isolate and purify the compound (4R, 7R, 14S)-4α, 7α-diacethoxy-14-hydroxydolastane-1(15), 8-dien. Then, the effects of the extract and the dolastane diterpene on zygotes and gametes of the sea urchin Lytechinus variegatus were evaluated. The exposure of male and female gametes to the C. cervicornis extract promoted, respectively, a reduction of 10-30% in fertilization and a 20 to 70% decrease in the number of eggs. Furthermore, the exposure of zygotes to the extract inhibited their development up to 86.7 ± 1.6% (at a concentration of 250 µg mL-1), as well as generating abnormalities in 39-50% of zygotes. The results of the dolastane diterpene showed no evidence of inhibition in the zygotes' development, thought it was proved to induce anomalies. At higher concentrations (25 and 50 µg mL-1), it was possible to observe cell lyses.
author	Ortiz-Ramírez,Fredy A Aparecida-Vallim,Magui Negrão-Cavalcanti,Diana Laneuville-Teixeira,Valéria
author_facet	Ortiz-Ramírez,Fredy A Aparecida-Vallim,Magui Negrão-Cavalcanti,Diana Laneuville-Teixeira,Valéria
author_sort	Ortiz-Ramírez,Fredy A
title	Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin Lytechinus
title_short	Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin Lytechinus
title_full	Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin Lytechinus
title_fullStr	Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin Lytechinus
title_full_unstemmed	Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin Lytechinus
title_sort	effects of the secondary metabolites from canistrocarpus cervicornis (dictyotales, phaeophyceae) on fertilization and early development of the sea urchin lytechinus
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000200008
work_keys_str_mv	AT ortizramirezfredya effectsofthesecondarymetabolitesfromcanistrocarpuscervicornisdictyotalesphaeophyceaeonfertilizationandearlydevelopmentoftheseaurchinlytechinus AT aparecidavallimmagui effectsofthesecondarymetabolitesfromcanistrocarpuscervicornisdictyotalesphaeophyceaeonfertilizationandearlydevelopmentoftheseaurchinlytechinus AT negraocavalcantidiana effectsofthesecondarymetabolitesfromcanistrocarpuscervicornisdictyotalesphaeophyceaeonfertilizationandearlydevelopmentoftheseaurchinlytechinus AT laneuvilleteixeiravaleria effectsofthesecondarymetabolitesfromcanistrocarpuscervicornisdictyotalesphaeophyceaeonfertilizationandearlydevelopmentoftheseaurchinlytechinus
_version_	1714205107539673088

Effects of the secondary metabolites from Canistrocarpus cervicornis (Dictyotales, Phaeophyceae) on fertilization and early development of the sea urchin Lytechinus

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