Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of Brazil

Previous studies demonstrated that the crude extract of the ascidian Eudistoma vannamei, endemic from northeasttern Brazil, strongly hinders growth of tumor cells in vitro by inducing apoptosis due to tryptophan derivatives, which are commonly found in bacteria. This study presents a bioactivity-gui...

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Autores principales:	Jimenez,Paula C, Ferreira,Elthon G, Araújo,Luana A, Guimarães,Larissa A, Sousa,Thiciana S, Pessoa,Otília Deusdenia L, Lotufo,Tito M. C, Costa-Lotufo,Letícia V
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2013
Materias:	marine biotechnology Eudistoma vannamei marine microorganisms Micromonospora sp Brazil
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000200012
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spelling	oai:scielo:S0718-560X20130002000122013-08-13Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of BrazilJimenez,Paula CFerreira,Elthon GAraújo,Luana AGuimarães,Larissa ASousa,Thiciana SPessoa,Otília Deusdenia LLotufo,Tito M. CCosta-Lotufo,Letícia V marine biotechnology Eudistoma vannamei marine microorganisms Micromonospora sp Brazil Previous studies demonstrated that the crude extract of the ascidian Eudistoma vannamei, endemic from northeasttern Brazil, strongly hinders growth of tumor cells in vitro by inducing apoptosis due to tryptophan derivatives, which are commonly found in bacteria. This study presents a bioactivity-guided screening among actinomycetes, associated with E. vannamei, aiming at recognizing active principles with biological relevance. Twenty strains of actinomycetes, designated as EVA 0101 through 0120, were isolated from colonies of E. vannamei among which 11 were selected for cytotoxicity evaluation. The extracts from EVA 0102, 0103, 0106, 0109 and 0113 were the most active, and were further studied for IC50 determination and chemical analysis by ¹H NMR. IC50 values obtained ranged from 3.62 µg mL-1 (for EVA 0109 in leukemia cells) to 84.65 µg/mL (for EVA 0106 in melanoma cells). All active extracts exhibited the same TLC and spectroscopic profiles, suggesting the presence of quinones and other related secondary metabolites. Furthermore, these strains were identified and compared based on their respective 16S rRNA sequences. The results herein identified the five strains as Micromonospora spp. while phylogenetic analysis suggests that they are possibly two different Micromonospora species producing the cytotoxic compounds.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.41 n.2 20132013-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000200012en10.3856/vol41-issue2-fulltext-12
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	marine biotechnology Eudistoma vannamei marine microorganisms Micromonospora sp Brazil
spellingShingle	marine biotechnology Eudistoma vannamei marine microorganisms Micromonospora sp Brazil Jimenez,Paula C Ferreira,Elthon G Araújo,Luana A Guimarães,Larissa A Sousa,Thiciana S Pessoa,Otília Deusdenia L Lotufo,Tito M. C Costa-Lotufo,Letícia V Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of Brazil
description	Previous studies demonstrated that the crude extract of the ascidian Eudistoma vannamei, endemic from northeasttern Brazil, strongly hinders growth of tumor cells in vitro by inducing apoptosis due to tryptophan derivatives, which are commonly found in bacteria. This study presents a bioactivity-guided screening among actinomycetes, associated with E. vannamei, aiming at recognizing active principles with biological relevance. Twenty strains of actinomycetes, designated as EVA 0101 through 0120, were isolated from colonies of E. vannamei among which 11 were selected for cytotoxicity evaluation. The extracts from EVA 0102, 0103, 0106, 0109 and 0113 were the most active, and were further studied for IC50 determination and chemical analysis by ¹H NMR. IC50 values obtained ranged from 3.62 µg mL-1 (for EVA 0109 in leukemia cells) to 84.65 µg/mL (for EVA 0106 in melanoma cells). All active extracts exhibited the same TLC and spectroscopic profiles, suggesting the presence of quinones and other related secondary metabolites. Furthermore, these strains were identified and compared based on their respective 16S rRNA sequences. The results herein identified the five strains as Micromonospora spp. while phylogenetic analysis suggests that they are possibly two different Micromonospora species producing the cytotoxic compounds.
author	Jimenez,Paula C Ferreira,Elthon G Araújo,Luana A Guimarães,Larissa A Sousa,Thiciana S Pessoa,Otília Deusdenia L Lotufo,Tito M. C Costa-Lotufo,Letícia V
author_facet	Jimenez,Paula C Ferreira,Elthon G Araújo,Luana A Guimarães,Larissa A Sousa,Thiciana S Pessoa,Otília Deusdenia L Lotufo,Tito M. C Costa-Lotufo,Letícia V
author_sort	Jimenez,Paula C
title	Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of Brazil
title_short	Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of Brazil
title_full	Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of Brazil
title_fullStr	Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of Brazil
title_full_unstemmed	Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of Brazil
title_sort	cytotoxicity of actinomycetes associated with the ascidian eudistoma vannamei (millar, 1977), endemic of northeastern coast of brazil
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000200012
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Cytotoxicity of actinomycetes associated with the ascidian Eudistoma vannamei (Millar, 1977), endemic of northeastern coast of Brazil

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