Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture system

The yellowtail (Seriola lalandi) has been farmed for many years and is becoming a promising aquaculture species. Knowledge of the intestinal microbiota of this species is very limited. Thus, the aim of this study is to describe the bacterial populations associated with the intestinal tract of Seriol...

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Autores principales:	Aguilera,Eduardo, Yany,Gabriel, Romero,Jaime
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2013
Materias:	intestinal microbiota 16S rRNA Seriola lalandi yellowtail seawater aquaculture Chile
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000300003
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spelling	oai:scielo:S0718-560X20130003000032013-10-15Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture systemAguilera,EduardoYany,GabrielRomero,Jaime intestinal microbiota 16S rRNA Seriola lalandi yellowtail seawater aquaculture Chile The yellowtail (Seriola lalandi) has been farmed for many years and is becoming a promising aquaculture species. Knowledge of the intestinal microbiota of this species is very limited. Thus, the aim of this study is to describe the bacterial populations associated with the intestinal tract of Seriola lalandi reared in Chile. The microbiota composition was analyzed at two growth stages distinguished by weight and parameters such as Specific Growth Rate (SGR) and Feed Conversion Ratio (FCR). Juveniles (mean initial weight 7.33 ± 0.30 g) and pre-adults (81.7 ± 19.0 g) were fed with commercial diet for 33 and 50 days, respectively. The first intestinal samples were collected at the end of Trial 1 from specimens weighing approximately 50 g while the second samples were obtained at the end of Trial 2 from specimens weighing approximately 370 g. The microbiota composition was examined using conventional isolation in Tryptic Soy Agar (TSA) followed by 16S rRNA sequencing and identification. In total, 16 genera were identified. Pseudomonas, Vibrio and Staphylococcus were the predominant genera in fish at the 50 g stage, whereas Microbacterium and Francisella were the predominant genera in the 370 g stage. The microbiota composition showed different assemblages, depending on host size, with Bacillus and Vibrio being the only genera that were shared. Knowledge of the intestinal microbiota of Seriola lalandi is the first step in the exploration of microbiota management and the development of probiotics, as well as in the identification of the bacterial populations in healthy fish under cultured conditions.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.41 n.3 20132013-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000300003en103856/vol41-issue3-fulltext-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	intestinal microbiota 16S rRNA Seriola lalandi yellowtail seawater aquaculture Chile
spellingShingle	intestinal microbiota 16S rRNA Seriola lalandi yellowtail seawater aquaculture Chile Aguilera,Eduardo Yany,Gabriel Romero,Jaime Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture system
description	The yellowtail (Seriola lalandi) has been farmed for many years and is becoming a promising aquaculture species. Knowledge of the intestinal microbiota of this species is very limited. Thus, the aim of this study is to describe the bacterial populations associated with the intestinal tract of Seriola lalandi reared in Chile. The microbiota composition was analyzed at two growth stages distinguished by weight and parameters such as Specific Growth Rate (SGR) and Feed Conversion Ratio (FCR). Juveniles (mean initial weight 7.33 ± 0.30 g) and pre-adults (81.7 ± 19.0 g) were fed with commercial diet for 33 and 50 days, respectively. The first intestinal samples were collected at the end of Trial 1 from specimens weighing approximately 50 g while the second samples were obtained at the end of Trial 2 from specimens weighing approximately 370 g. The microbiota composition was examined using conventional isolation in Tryptic Soy Agar (TSA) followed by 16S rRNA sequencing and identification. In total, 16 genera were identified. Pseudomonas, Vibrio and Staphylococcus were the predominant genera in fish at the 50 g stage, whereas Microbacterium and Francisella were the predominant genera in the 370 g stage. The microbiota composition showed different assemblages, depending on host size, with Bacillus and Vibrio being the only genera that were shared. Knowledge of the intestinal microbiota of Seriola lalandi is the first step in the exploration of microbiota management and the development of probiotics, as well as in the identification of the bacterial populations in healthy fish under cultured conditions.
author	Aguilera,Eduardo Yany,Gabriel Romero,Jaime
author_facet	Aguilera,Eduardo Yany,Gabriel Romero,Jaime
author_sort	Aguilera,Eduardo
title	Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture system
title_short	Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture system
title_full	Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture system
title_fullStr	Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture system
title_full_unstemmed	Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture system
title_sort	cultivable intestinal microbiota of yellowtail juveniles (seriola lalandi) in an aquaculture system
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000300003
work_keys_str_mv	AT aguileraeduardo cultivableintestinalmicrobiotaofyellowtailjuvenilesseriolalalandiinanaquaculturesystem AT yanygabriel cultivableintestinalmicrobiotaofyellowtailjuvenilesseriolalalandiinanaquaculturesystem AT romerojaime cultivableintestinalmicrobiotaofyellowtailjuvenilesseriolalalandiinanaquaculturesystem
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Cultivable intestinal microbiota of yellowtail juveniles (Seriola lalandi) in an aquaculture system

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