Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high interti...

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Autores principales:	Contreras-Porcia,Loretto, López-Cristoffanini,Camilo, Lovazzano,Carlos, Flores-Molina,María Rosa, Thomas,Daniela, Núñez,Alejandra, Fierro,Camila, Guajardo,Eduardo, Correa,Juan A, Kube,Michael, Reinhardt,Richard
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2013
Materias:	Pyropia desiccation stress ESTs seaweeds transcriptomics proteins
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000500013
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spelling	oai:scielo:S0718-560X20130005000132014-04-23Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditionsContreras-Porcia,LorettoLópez-Cristoffanini,CamiloLovazzano,CarlosFlores-Molina,María RosaThomas,DanielaNúñez,AlejandraFierro,CamilaGuajardo,EduardoCorrea,Juan AKube,MichaelReinhardt,Richard Pyropia desiccation stress ESTs seaweeds transcriptomics proteins In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.41 n.5 20132013-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000500013en103856/vol41-issue5-fulltext-13
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pyropia desiccation stress ESTs seaweeds transcriptomics proteins
spellingShingle	Pyropia desiccation stress ESTs seaweeds transcriptomics proteins Contreras-Porcia,Loretto López-Cristoffanini,Camilo Lovazzano,Carlos Flores-Molina,María Rosa Thomas,Daniela Núñez,Alejandra Fierro,Camila Guajardo,Eduardo Correa,Juan A Kube,Michael Reinhardt,Richard Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
description	In rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.
author	Contreras-Porcia,Loretto López-Cristoffanini,Camilo Lovazzano,Carlos Flores-Molina,María Rosa Thomas,Daniela Núñez,Alejandra Fierro,Camila Guajardo,Eduardo Correa,Juan A Kube,Michael Reinhardt,Richard
author_facet	Contreras-Porcia,Loretto López-Cristoffanini,Camilo Lovazzano,Carlos Flores-Molina,María Rosa Thomas,Daniela Núñez,Alejandra Fierro,Camila Guajardo,Eduardo Correa,Juan A Kube,Michael Reinhardt,Richard
author_sort	Contreras-Porcia,Loretto
title	Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
title_short	Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
title_full	Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
title_fullStr	Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
title_full_unstemmed	Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions
title_sort	differential gene expression in pyropia columbina (bangiales, rhodophyta) under natural hydration and desiccation conditions
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000500013
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Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

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