Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimens
Many of the characteristics used in sea anemone taxonomy can only be examined through histological sections. Since there is no standardized procedure for this purpose, various anesthesia and fixation techniques applied to specimens of the intertidal species Anthopleura hermaphroditica and Bunodactis...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
2013
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oai:scielo:S0718-560X20130005000232014-04-23Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimensSpano,CarlosFlores,Verónica Actiniidae Anthopleura Bunodactis Masson's trichrome histology Many of the characteristics used in sea anemone taxonomy can only be examined through histological sections. Since there is no standardized procedure for this purpose, various anesthesia and fixation techniques applied to specimens of the intertidal species Anthopleura hermaphroditica and Bunodactis hermafroditica are discussed. Additionally, further modifications are proposed to the Masson's trichrome method according to the results obtained on these species. The combined effect of the short application of menthol crystals, together with small doses of MgCl2 were the most satisfactory anesthetics for maintaining the specimens expanded. The best preparations were obtained from samples fixed for several months in 8% seawater formalin; however, in order to achieve a good differentiation of the tissue, mordanting the samples with Bouin's fixative was necessary. Besides being a fast method, the modified Masson's trichrome gives very good contrasts between the epithelia and the mesoglea, and allows controlling the timing of differentiation during staining. The present paper includes suggestions and precautions and thus offers practical help for the histological study of sea anemones.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.41 n.5 20132013-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000500023en103856/vol41-issue5-fulltext-23 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Actiniidae Anthopleura Bunodactis Masson's trichrome histology |
| spellingShingle |
Actiniidae Anthopleura Bunodactis Masson's trichrome histology Spano,Carlos Flores,Verónica Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimens |
| description |
Many of the characteristics used in sea anemone taxonomy can only be examined through histological sections. Since there is no standardized procedure for this purpose, various anesthesia and fixation techniques applied to specimens of the intertidal species Anthopleura hermaphroditica and Bunodactis hermafroditica are discussed. Additionally, further modifications are proposed to the Masson's trichrome method according to the results obtained on these species. The combined effect of the short application of menthol crystals, together with small doses of MgCl2 were the most satisfactory anesthetics for maintaining the specimens expanded. The best preparations were obtained from samples fixed for several months in 8% seawater formalin; however, in order to achieve a good differentiation of the tissue, mordanting the samples with Bouin's fixative was necessary. Besides being a fast method, the modified Masson's trichrome gives very good contrasts between the epithelia and the mesoglea, and allows controlling the timing of differentiation during staining. The present paper includes suggestions and precautions and thus offers practical help for the histological study of sea anemones. |
| author |
Spano,Carlos Flores,Verónica |
| author_facet |
Spano,Carlos Flores,Verónica |
| author_sort |
Spano,Carlos |
| title |
Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimens |
| title_short |
Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimens |
| title_full |
Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimens |
| title_fullStr |
Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimens |
| title_full_unstemmed |
Staining protocol for the histological study of sea anemones (Anthozoa: Actiniaria) with recommendations for anesthesia and fixation of specimens |
| title_sort |
staining protocol for the histological study of sea anemones (anthozoa: actiniaria) with recommendations for anesthesia and fixation of specimens |
| publisher |
Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar |
| publishDate |
2013 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2013000500023 |
| work_keys_str_mv |
AT spanocarlos stainingprotocolforthehistologicalstudyofseaanemonesanthozoaactiniariawithrecommendationsforanesthesiaandfixationofspecimens AT floresveronica stainingprotocolforthehistologicalstudyofseaanemonesanthozoaactiniariawithrecommendationsforanesthesiaandfixationofspecimens |
| _version_ |
1714205119227101184 |