Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil

The shrimp Litopenaeus vannamei, one of the most important species in world aquaculture, has seriously affected by infectious myonecrosis virus (IMNV) that causes up to 70% mortalities. With the aim of improving the development of new strategies for rapid and reliable diagnosis, we isolated IMNV, fr...

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Autores principales:	Coelho-Melo,Maria Verônyca, Florindo-Guedes,Maria Izabel, Rodriguez-Málaga,Sergio, Magalhães de Almeida,Lia, de Freitas Moreira,Mariana, Rodrigues de Oliveira,Tatiane
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2014
Materias:	Litopenaeus vannamei IMNV RNA RT-PCR sequencing aquaculture Brazil
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2014000300022
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spelling	oai:scielo:S0718-560X20140003000222014-10-10Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, BrazilCoelho-Melo,Maria VerônycaFlorindo-Guedes,Maria IzabelRodriguez-Málaga,SergioMagalhães de Almeida,Liade Freitas Moreira,MarianaRodrigues de Oliveira,Tatiane Litopenaeus vannamei IMNV RNA RT-PCR sequencing aquaculture Brazil The shrimp Litopenaeus vannamei, one of the most important species in world aquaculture, has seriously affected by infectious myonecrosis virus (IMNV) that causes up to 70% mortalities. With the aim of improving the development of new strategies for rapid and reliable diagnosis, we isolated IMNV, from L. vannamei farmed in Brazil, through a discontinuous sucrose gradient, and sequenced cDNA fragment encoding the major capsid protein from this virus. Nucleotides sequences corresponding to the viral capsid protein was obtained by RT-PCR and confirmed by automatic sequencing. Comparison with sequences which encode the capsid protein obtained from Indonesia isolates showed a high identity.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.42 n.3 20142014-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2014000300022en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Litopenaeus vannamei IMNV RNA RT-PCR sequencing aquaculture Brazil
spellingShingle	Litopenaeus vannamei IMNV RNA RT-PCR sequencing aquaculture Brazil Coelho-Melo,Maria Verônyca Florindo-Guedes,Maria Izabel Rodriguez-Málaga,Sergio Magalhães de Almeida,Lia de Freitas Moreira,Mariana Rodrigues de Oliveira,Tatiane Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil
description	The shrimp Litopenaeus vannamei, one of the most important species in world aquaculture, has seriously affected by infectious myonecrosis virus (IMNV) that causes up to 70% mortalities. With the aim of improving the development of new strategies for rapid and reliable diagnosis, we isolated IMNV, from L. vannamei farmed in Brazil, through a discontinuous sucrose gradient, and sequenced cDNA fragment encoding the major capsid protein from this virus. Nucleotides sequences corresponding to the viral capsid protein was obtained by RT-PCR and confirmed by automatic sequencing. Comparison with sequences which encode the capsid protein obtained from Indonesia isolates showed a high identity.
author	Coelho-Melo,Maria Verônyca Florindo-Guedes,Maria Izabel Rodriguez-Málaga,Sergio Magalhães de Almeida,Lia de Freitas Moreira,Mariana Rodrigues de Oliveira,Tatiane
author_facet	Coelho-Melo,Maria Verônyca Florindo-Guedes,Maria Izabel Rodriguez-Málaga,Sergio Magalhães de Almeida,Lia de Freitas Moreira,Mariana Rodrigues de Oliveira,Tatiane
author_sort	Coelho-Melo,Maria Verônyca
title	Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil
title_short	Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil
title_full	Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil
title_fullStr	Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil
title_full_unstemmed	Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil
title_sort	molecular characterization of infectious myonecrosis virus (imnv) isolated from the shrimp litopenaeus vannamei farmed in ceará state, brazil
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2014000300022
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Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil

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