Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures

The cell and culture viability of concentrates of the microalga Nannochloropsis oculata were assessed after storage for a period of 16 weeks at -18, 0 and 5°C. The concentrates were obtained from the crop of N. oculata, which was harvested at the start of the seasonal growth period using a process o...

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Autores principales:	Low,Constanza, Toledo,María Isabel
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2015
Materias:	Nannochloropsis oculata shelf-life flocculation cell viability aquaculture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000200007
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spelling	oai:scielo:S0718-560X20150002000072015-06-01Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperaturesLow,ConstanzaToledo,María Isabel Nannochloropsis oculata shelf-life flocculation cell viability aquaculture The cell and culture viability of concentrates of the microalga Nannochloropsis oculata were assessed after storage for a period of 16 weeks at -18, 0 and 5°C. The concentrates were obtained from the crop of N. oculata, which was harvested at the start of the seasonal growth period using a process of flocculation. Flocotac Plus was used as the flocculation agent, achieving flocculation of 90% of the suspended microalgae. It was observed that the chemical process did not affect the number of live cells. The concentrate stored at -18°C presented slow freezing, which deteriorated the cells and therefore reduced cell viability after five weeks (75%). The concentrates stored at 0 and 5°C showed cell viability over 97% after the 16 weeks. Culture viability was only seen in the concentrates stored at 0 and 5°C, which showed specific growth rates similar to those of the control culture. It may be concluded that it is possible to use flocculates stored at 0 and 5°C after 16 weeks as inoculum for mass crops of N. oculata for food, green water and other uses.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.43 n.2 20152015-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000200007en10.3856/vol43-issue2-fulltext-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Nannochloropsis oculata shelf-life flocculation cell viability aquaculture
spellingShingle	Nannochloropsis oculata shelf-life flocculation cell viability aquaculture Low,Constanza Toledo,María Isabel Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures
description	The cell and culture viability of concentrates of the microalga Nannochloropsis oculata were assessed after storage for a period of 16 weeks at -18, 0 and 5°C. The concentrates were obtained from the crop of N. oculata, which was harvested at the start of the seasonal growth period using a process of flocculation. Flocotac Plus was used as the flocculation agent, achieving flocculation of 90% of the suspended microalgae. It was observed that the chemical process did not affect the number of live cells. The concentrate stored at -18°C presented slow freezing, which deteriorated the cells and therefore reduced cell viability after five weeks (75%). The concentrates stored at 0 and 5°C showed cell viability over 97% after the 16 weeks. Culture viability was only seen in the concentrates stored at 0 and 5°C, which showed specific growth rates similar to those of the control culture. It may be concluded that it is possible to use flocculates stored at 0 and 5°C after 16 weeks as inoculum for mass crops of N. oculata for food, green water and other uses.
author	Low,Constanza Toledo,María Isabel
author_facet	Low,Constanza Toledo,María Isabel
author_sort	Low,Constanza
title	Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures
title_short	Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures
title_full	Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures
title_fullStr	Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures
title_full_unstemmed	Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures
title_sort	assessment of the shelf-life of nannochloropsis oculata flocculates stored at different temperatures
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000200007
work_keys_str_mv	AT lowconstanza assessmentoftheshelflifeofnannochloropsisoculataflocculatesstoredatdifferenttemperatures AT toledomariaisabel assessmentoftheshelflifeofnannochloropsisoculataflocculatesstoredatdifferenttemperatures
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Assessment of the shelf-life of Nannochloropsis oculata flocculates stored at different temperatures

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