Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)

Partial characterizations of digestive proteases were studied in three life stages of spotted rose snapper: early (EJ), middle (MJ) and late juvenile (LJ) with corresponding average weights of 21.3 ± 2.6 g (3 months after hatching, MAH), 190 ± 4.4 g (7 MAH), and 400 ± 11.5 g (12 MAH). At sampling po...

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Autores principales:	Peña,Emyr, Hernández,Crisantema, Álvarez-González,Carlos Alfonso, Ibarra-Castro,Leonardo, Puello-Cruz,Ana, Hardy,Ronald W
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2015
Materias:	Lutjanus guttatus spotted rose snapper digestive enzymes pepsin trypsin protease inhibitors
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400003
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spelling	oai:scielo:S0718-560X20150004000032015-12-04Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)Peña,EmyrHernández,CrisantemaÁlvarez-González,Carlos AlfonsoIbarra-Castro,LeonardoPuello-Cruz,AnaHardy,Ronald W Lutjanus guttatus spotted rose snapper digestive enzymes pepsin trypsin protease inhibitors Partial characterizations of digestive proteases were studied in three life stages of spotted rose snapper: early (EJ), middle (MJ) and late juvenile (LJ) with corresponding average weights of 21.3 ± 2.6 g (3 months after hatching, MAH), 190 ± 4.4 g (7 MAH), and 400 ± 11.5 g (12 MAH). At sampling points, the digestive tract was dissected into the stomach (St), pyloric caeca (PC), and the intestine in three sections (proximal (PI), middle (MI) and distal intestine (DI)). The effect of pH and temperature and specific inhibitors were evaluated for acid and alkaline proteases. Total acid and alkaline protease activity showed a tendency to increase with juvenile life stage of fish while trypsin activity decreased. Differences were found in acid and alkaline protease activities at different pH and temperatures during juvenile stages. Pepstatin A inhibited total activity in the stomach extract in all juvenile stages. Activity in total alkaline protease inhibition was significantly higher in EJ using TLCK, PMSF, SBTI, Phen and Ovo than in MJ and LJ, while no significant differences were found with TPCK inhibition. Therefore increases in protease activities with fish growth through juvenile stages in which a substitution or diversification in the type of alkaline enzymes exist. These results lead a better comprehension of changes in digestive potential of Lutjanidae fish.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.43 n.4 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400003en10.3856/vol43-issue4-fulltext-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lutjanus guttatus spotted rose snapper digestive enzymes pepsin trypsin protease inhibitors
spellingShingle	Lutjanus guttatus spotted rose snapper digestive enzymes pepsin trypsin protease inhibitors Peña,Emyr Hernández,Crisantema Álvarez-González,Carlos Alfonso Ibarra-Castro,Leonardo Puello-Cruz,Ana Hardy,Ronald W Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)
description	Partial characterizations of digestive proteases were studied in three life stages of spotted rose snapper: early (EJ), middle (MJ) and late juvenile (LJ) with corresponding average weights of 21.3 ± 2.6 g (3 months after hatching, MAH), 190 ± 4.4 g (7 MAH), and 400 ± 11.5 g (12 MAH). At sampling points, the digestive tract was dissected into the stomach (St), pyloric caeca (PC), and the intestine in three sections (proximal (PI), middle (MI) and distal intestine (DI)). The effect of pH and temperature and specific inhibitors were evaluated for acid and alkaline proteases. Total acid and alkaline protease activity showed a tendency to increase with juvenile life stage of fish while trypsin activity decreased. Differences were found in acid and alkaline protease activities at different pH and temperatures during juvenile stages. Pepstatin A inhibited total activity in the stomach extract in all juvenile stages. Activity in total alkaline protease inhibition was significantly higher in EJ using TLCK, PMSF, SBTI, Phen and Ovo than in MJ and LJ, while no significant differences were found with TPCK inhibition. Therefore increases in protease activities with fish growth through juvenile stages in which a substitution or diversification in the type of alkaline enzymes exist. These results lead a better comprehension of changes in digestive potential of Lutjanidae fish.
author	Peña,Emyr Hernández,Crisantema Álvarez-González,Carlos Alfonso Ibarra-Castro,Leonardo Puello-Cruz,Ana Hardy,Ronald W
author_facet	Peña,Emyr Hernández,Crisantema Álvarez-González,Carlos Alfonso Ibarra-Castro,Leonardo Puello-Cruz,Ana Hardy,Ronald W
author_sort	Peña,Emyr
title	Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)
title_short	Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)
title_full	Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)
title_fullStr	Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)
title_full_unstemmed	Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)
title_sort	comparative characterization of protease activity in cultured spotted rose snapper juveniles (lutjanus guttatus)
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400003
work_keys_str_mv	AT penaemyr comparativecharacterizationofproteaseactivityinculturedspottedrosesnapperjuvenileslutjanusguttatus AT hernandezcrisantema comparativecharacterizationofproteaseactivityinculturedspottedrosesnapperjuvenileslutjanusguttatus AT alvarezgonzalezcarlosalfonso comparativecharacterizationofproteaseactivityinculturedspottedrosesnapperjuvenileslutjanusguttatus AT ibarracastroleonardo comparativecharacterizationofproteaseactivityinculturedspottedrosesnapperjuvenileslutjanusguttatus AT puellocruzana comparativecharacterizationofproteaseactivityinculturedspottedrosesnapperjuvenileslutjanusguttatus AT hardyronaldw comparativecharacterizationofproteaseactivityinculturedspottedrosesnapperjuvenileslutjanusguttatus
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Comparative characterization of protease activity in cultured spotted rose snapper juveniles (Lutjanus guttatus)

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