Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170

The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single stra...

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Autores principales:	Luis-Villaseñor,Irasema E, Voltolina,Domenico, Gomez-Gil,Bruno, Ascencio,Felipe, Campa-Córdova,Ángel I, Audelo-Naranjo,Juan M, Zamudio-Armenta,Olga O
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2015
Materias:	Litopenaeus vannamei Bacillus mix Vibrio parahaemolyticus bacterial community aquaculture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400015
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spelling	oai:scielo:S0718-560X20150004000152015-12-04Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170Luis-Villaseñor,Irasema EVoltolina,DomenicoGomez-Gil,BrunoAscencio,FelipeCampa-Córdova,Ángel IAudelo-Naranjo,Juan MZamudio-Armenta,Olga O Litopenaeus vannamei Bacillus mix Vibrio parahaemolyticus bacterial community aquaculture The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism) technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B) and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5*10(5) CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus) and negative (not injected) controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.43 n.4 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400015en10.3856/vol43-issue4-fulltext-15
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Litopenaeus vannamei Bacillus mix Vibrio parahaemolyticus bacterial community aquaculture
spellingShingle	Litopenaeus vannamei Bacillus mix Vibrio parahaemolyticus bacterial community aquaculture Luis-Villaseñor,Irasema E Voltolina,Domenico Gomez-Gil,Bruno Ascencio,Felipe Campa-Córdova,Ángel I Audelo-Naranjo,Juan M Zamudio-Armenta,Olga O Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170
description	The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism) technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B) and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5*10(5) CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus) and negative (not injected) controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp.
author	Luis-Villaseñor,Irasema E Voltolina,Domenico Gomez-Gil,Bruno Ascencio,Felipe Campa-Córdova,Ángel I Audelo-Naranjo,Juan M Zamudio-Armenta,Olga O
author_facet	Luis-Villaseñor,Irasema E Voltolina,Domenico Gomez-Gil,Bruno Ascencio,Felipe Campa-Córdova,Ángel I Audelo-Naranjo,Juan M Zamudio-Armenta,Olga O
author_sort	Luis-Villaseñor,Irasema E
title	Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170
title_short	Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170
title_full	Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170
title_fullStr	Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170
title_full_unstemmed	Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170
title_sort	probiotic modulation of the gut bacterial community of juvenile litopenaeus vannamei challenged with vibrio parahaemolyticus caim 170
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400015
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Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170

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