Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms

Recent field and experimental evidence collected in the southern upwelling region off Concepción (36°5'S, 73°3'W) showed an abrupt reduction (<72 h) in the egg production rates (EPR) of copepods when they were fed steadily and solely with the local bloom-forming diatom Thalassiosira rot...

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Autores principales:	Aguilera,Victor M, Escribano,Rubén, Martínez-Oyanedel,José
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2015
Materias:	diatoms blooms food copepods reproduction protein profiles
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400020
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spelling	oai:scielo:S0718-560X20150004000202015-12-04Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatomsAguilera,Victor MEscribano,RubénMartínez-Oyanedel,José diatoms blooms food copepods reproduction protein profiles Recent field and experimental evidence collected in the southern upwelling region off Concepción (36°5'S, 73°3'W) showed an abrupt reduction (<72 h) in the egg production rates (EPR) of copepods when they were fed steadily and solely with the local bloom-forming diatom Thalassiosira rotula. Because diatoms were biochemically similar to dinoflagellate Prorocentrum minimum, a diet which supported higher reproductive outcomes, the fecundity reduction observed in copepod females fed with the diatom may have obeyed to post-ingestive processes, giving rise to resources reallocation. This hypothesis was tested by comparing feeding (clearance and ingestion rates), reproduction (EPR and hatching success) and the structure of protein profiles (i.e., number and intensity of electrophoretic bands) of copepods (adults and eggs) incubated during 96 h with the two food conditions. The structure of protein profiles included molecular sizes that were calculated from the relative mobility of protein standards against the logarithm of their molecular sizes. After assessing the experimental conditions, feeding decreased over time for those females fed with T. rotula, while reproduction was higher in females fed with P. minimum. Electrophoretic profiles resulted similar mostly at a banding region of 100 to 89-kDa, while they showed partial differences around the region of 56-kDa band, especially in those females fed and eggs produced with T. rotula. Due to reproductive volume was impacted while larvae viability, a physiological processes with specific and high nutritional requirements, was independent on food type; post-ingestive processes, such as expression of stress-related proteins deviating resources to metabolic processes others than reproduction, are discussed under framework of nutritional-toxic mechanisms mediating copepod-diatoms relationships in productive upwelling areas.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.43 n.4 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400020en10.3856/vol43-issue4-fulltext-20
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	diatoms blooms food copepods reproduction protein profiles
spellingShingle	diatoms blooms food copepods reproduction protein profiles Aguilera,Victor M Escribano,Rubén Martínez-Oyanedel,José Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms
description	Recent field and experimental evidence collected in the southern upwelling region off Concepción (36°5'S, 73°3'W) showed an abrupt reduction (<72 h) in the egg production rates (EPR) of copepods when they were fed steadily and solely with the local bloom-forming diatom Thalassiosira rotula. Because diatoms were biochemically similar to dinoflagellate Prorocentrum minimum, a diet which supported higher reproductive outcomes, the fecundity reduction observed in copepod females fed with the diatom may have obeyed to post-ingestive processes, giving rise to resources reallocation. This hypothesis was tested by comparing feeding (clearance and ingestion rates), reproduction (EPR and hatching success) and the structure of protein profiles (i.e., number and intensity of electrophoretic bands) of copepods (adults and eggs) incubated during 96 h with the two food conditions. The structure of protein profiles included molecular sizes that were calculated from the relative mobility of protein standards against the logarithm of their molecular sizes. After assessing the experimental conditions, feeding decreased over time for those females fed with T. rotula, while reproduction was higher in females fed with P. minimum. Electrophoretic profiles resulted similar mostly at a banding region of 100 to 89-kDa, while they showed partial differences around the region of 56-kDa band, especially in those females fed and eggs produced with T. rotula. Due to reproductive volume was impacted while larvae viability, a physiological processes with specific and high nutritional requirements, was independent on food type; post-ingestive processes, such as expression of stress-related proteins deviating resources to metabolic processes others than reproduction, are discussed under framework of nutritional-toxic mechanisms mediating copepod-diatoms relationships in productive upwelling areas.
author	Aguilera,Victor M Escribano,Rubén Martínez-Oyanedel,José
author_facet	Aguilera,Victor M Escribano,Rubén Martínez-Oyanedel,José
author_sort	Aguilera,Victor M
title	Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms
title_short	Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms
title_full	Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms
title_fullStr	Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms
title_full_unstemmed	Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms
title_sort	electrophoretic protein profiles of mid-sized copepod calanoides patagoniensis steadily fed bloom-forming diatoms
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2015000400020
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Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms

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