Immobilization of marine toxins on carboxylic acid modified surfaces

ABSTRACT. Saxitoxin and gonyautoxin 2 and 3 are among the most toxic components of the Paralytic Shellfish Poison from red tides. Being small molecules, they often require to be immobilized in order to be handled experimentally. Here is presented a methodology for covalently binding the toxins to ca...

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Autores principales:	Bustos,Paulina, Gaete,Diana, Villalobos,Patricio, Conejeros,Pablo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2016
Materias:	PSP ELISA magnetic beads plasmon resonance saxitoxin gonyautoxin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000100022
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spelling	oai:scielo:S0718-560X20160001000222016-05-27Immobilization of marine toxins on carboxylic acid modified surfacesBustos,PaulinaGaete,DianaVillalobos,PatricioConejeros,Pablo PSP ELISA magnetic beads plasmon resonance saxitoxin gonyautoxin ABSTRACT. Saxitoxin and gonyautoxin 2 and 3 are among the most toxic components of the Paralytic Shellfish Poison from red tides. Being small molecules, they often require to be immobilized in order to be handled experimentally. Here is presented a methodology for covalently binding the toxins to carboxilate-modified surfaces. Both toxins were successfully bound to magnetic beads and saxitoxin was additionally bound to a modified golden surface in order to perform a surface plasmon resonance analysis. Success of binding to magnetic beads was evaluated through a standard immune-based toxin assay. Despite the different methods used for each toxin, the maximum binding yield for both toxins occurred when using concentration of 120 µM.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.44 n.1 20162016-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000100022en10.3856/vol44-issue1-fulltext-22
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	PSP ELISA magnetic beads plasmon resonance saxitoxin gonyautoxin
spellingShingle	PSP ELISA magnetic beads plasmon resonance saxitoxin gonyautoxin Bustos,Paulina Gaete,Diana Villalobos,Patricio Conejeros,Pablo Immobilization of marine toxins on carboxylic acid modified surfaces
description	ABSTRACT. Saxitoxin and gonyautoxin 2 and 3 are among the most toxic components of the Paralytic Shellfish Poison from red tides. Being small molecules, they often require to be immobilized in order to be handled experimentally. Here is presented a methodology for covalently binding the toxins to carboxilate-modified surfaces. Both toxins were successfully bound to magnetic beads and saxitoxin was additionally bound to a modified golden surface in order to perform a surface plasmon resonance analysis. Success of binding to magnetic beads was evaluated through a standard immune-based toxin assay. Despite the different methods used for each toxin, the maximum binding yield for both toxins occurred when using concentration of 120 µM.
author	Bustos,Paulina Gaete,Diana Villalobos,Patricio Conejeros,Pablo
author_facet	Bustos,Paulina Gaete,Diana Villalobos,Patricio Conejeros,Pablo
author_sort	Bustos,Paulina
title	Immobilization of marine toxins on carboxylic acid modified surfaces
title_short	Immobilization of marine toxins on carboxylic acid modified surfaces
title_full	Immobilization of marine toxins on carboxylic acid modified surfaces
title_fullStr	Immobilization of marine toxins on carboxylic acid modified surfaces
title_full_unstemmed	Immobilization of marine toxins on carboxylic acid modified surfaces
title_sort	immobilization of marine toxins on carboxylic acid modified surfaces
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000100022
work_keys_str_mv	AT bustospaulina immobilizationofmarinetoxinsoncarboxylicacidmodifiedsurfaces AT gaetediana immobilizationofmarinetoxinsoncarboxylicacidmodifiedsurfaces AT villalobospatricio immobilizationofmarinetoxinsoncarboxylicacidmodifiedsurfaces AT conejerospablo immobilizationofmarinetoxinsoncarboxylicacidmodifiedsurfaces
_version_	1714205158670336000

Immobilization of marine toxins on carboxylic acid modified surfaces

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