Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)

Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND), was isolated from the hepatopancreas of moribund whiteleg shrimp of commercial farms from Guasave, Sinaloa, Mexico. The isolates were screened on thiosulfate citrate bile salt sucrose agar plates for the...

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Autores principales:	López-León,Patricia, Luna-González,Antonio, Escamilla-Montes,Ruth, Flores-Miranda,María del Carmen, Fierro-Coronado,Jesús A, Álvarez-Ruiz,Píndaro, Diarte-Plata,Genaro
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2016
Materias:	Litopenaeus vannamei Vibrio parahaemolyticus AHPND biofilm aquaculture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000300005
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spelling	oai:scielo:S0718-560X20160003000052016-10-03Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)López-León,PatriciaLuna-González,AntonioEscamilla-Montes,RuthFlores-Miranda,María del CarmenFierro-Coronado,Jesús AÁlvarez-Ruiz,PíndaroDiarte-Plata,Genaro Litopenaeus vannamei Vibrio parahaemolyticus AHPND biofilm aquaculture Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND), was isolated from the hepatopancreas of moribund whiteleg shrimp of commercial farms from Guasave, Sinaloa, Mexico. The isolates were screened on thiosulfate citrate bile salt sucrose agar plates for the selection of green colonies and further characterized through PCR with AP3 primers, 89F/R primers, hemolysin genes, hemolytic and enzymatic activity, hydrophobicity, autoaggregation, and biofilm formation. Bioassays by immersion challenge were conducted to confirm the pathogenicity of selected bacterial strains. In addition, the LC50 was calculated for each isolate. All isolates (35) belonged to V. parahaemolyticus, but three isolates did not correspond to strains that cause AHPND since they were negative with 89F/R primers. All isolates were a-hemolytic and showed biofilm formation (from moderate to strong). Isolates were hydrophobic or hydrophilic and showed high autoaggregation capacity. Eight strains did not kill shrimp and eleven were pathogenic, but differences in virulence were found among them perhaps due to α-hemolysis and differences in biofilm formation and hydrophobicity. Therefore, performed characterization may help to understand the pathogenicity of V. parahaemolyticus. Finally, results showed that smaller shrimp are less resistant to V. parahaemolyticus infection.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.44 n.3 20162016-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000300005en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Litopenaeus vannamei Vibrio parahaemolyticus AHPND biofilm aquaculture
spellingShingle	Litopenaeus vannamei Vibrio parahaemolyticus AHPND biofilm aquaculture López-León,Patricia Luna-González,Antonio Escamilla-Montes,Ruth Flores-Miranda,María del Carmen Fierro-Coronado,Jesús A Álvarez-Ruiz,Píndaro Diarte-Plata,Genaro Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)
description	Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND), was isolated from the hepatopancreas of moribund whiteleg shrimp of commercial farms from Guasave, Sinaloa, Mexico. The isolates were screened on thiosulfate citrate bile salt sucrose agar plates for the selection of green colonies and further characterized through PCR with AP3 primers, 89F/R primers, hemolysin genes, hemolytic and enzymatic activity, hydrophobicity, autoaggregation, and biofilm formation. Bioassays by immersion challenge were conducted to confirm the pathogenicity of selected bacterial strains. In addition, the LC50 was calculated for each isolate. All isolates (35) belonged to V. parahaemolyticus, but three isolates did not correspond to strains that cause AHPND since they were negative with 89F/R primers. All isolates were a-hemolytic and showed biofilm formation (from moderate to strong). Isolates were hydrophobic or hydrophilic and showed high autoaggregation capacity. Eight strains did not kill shrimp and eleven were pathogenic, but differences in virulence were found among them perhaps due to α-hemolysis and differences in biofilm formation and hydrophobicity. Therefore, performed characterization may help to understand the pathogenicity of V. parahaemolyticus. Finally, results showed that smaller shrimp are less resistant to V. parahaemolyticus infection.
author	López-León,Patricia Luna-González,Antonio Escamilla-Montes,Ruth Flores-Miranda,María del Carmen Fierro-Coronado,Jesús A Álvarez-Ruiz,Píndaro Diarte-Plata,Genaro
author_facet	López-León,Patricia Luna-González,Antonio Escamilla-Montes,Ruth Flores-Miranda,María del Carmen Fierro-Coronado,Jesús A Álvarez-Ruiz,Píndaro Diarte-Plata,Genaro
author_sort	López-León,Patricia
title	Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)
title_short	Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)
title_full	Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)
title_fullStr	Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)
title_full_unstemmed	Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)
title_sort	isolation and characterization of infectious vibrio parahaemolyticus, the causative agent of ahpnd, from the whiteleg shrimp (litopenaeus vannamei)
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000300005
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Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)

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