Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture

The microdot method is a downscaling methodology of traditional tenfold serial dilution procedure used in microbiology. The microdot method uses 100 µL for serial dilution and count colonies in a spot of 10 µL. In this study we counted colonies directly in a chromogenic agar plate to determine, at t...

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Autores principales:	Strahsburger,Erwin, Retamales,Patricio, Estrada,Juan, Seeger,Michael
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2016
Materias:	microdot method sanitary monitoring aquaculture chromogenic agar
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000400009
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spelling	oai:scielo:S0718-560X20160004000092016-10-28Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquacultureStrahsburger,ErwinRetamales,PatricioEstrada,JuanSeeger,Michael microdot method sanitary monitoring aquaculture chromogenic agar The microdot method is a downscaling methodology of traditional tenfold serial dilution procedure used in microbiology. The microdot method uses 100 µL for serial dilution and count colonies in a spot of 10 µL. In this study we counted colonies directly in a chromogenic agar plate to determine, at the same time, the presence and cell concentration of target bacteria required for sanitary monitoring of Chilean export fishery products. Due to among importers countries the most concerning bacteria included in sanitary monitoring are Escherichia coli, Listeria monocytogenes and Staphylococcus aureus, we used the chromogenic agar; CHROMagar ECC, CHROMagar Listeria and Baird Parker agar, respectively. The results shows no differences between quantitative results obtained with microdot and traditional method during the quantification of a culture of Escherichia coli (1.5 L). The sensitivity and specificity of the microdot method in association with each chromogenic agar was demonstrated in vitro with reference strains. In addition, the usefulness in sanitary monitoring of aquaculture procedures was evaluated in Artemia salina tanks. This method did not detected sanitary problems in surface water. Although other colonies grown in the chromogenic agar plate, their morphological and chromogenic properties not correspond to Escherichia coli, Listeria monocytogenes and Staphylococcus aureus, being identified as Salmonella enterica subsp. enterica, Microbacterium sp., Bacillus sp. and Staphylococcus pasteuri by 16S rRNA gene sequence analysis. Hence, we propose the microdot chromogenic method as a low cost, specific and reliable procedure for sanitary monitoring of aquaculture procedures.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.44 n.4 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000400009en10.3856/vol44-issue4-fulltext-9
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	microdot method sanitary monitoring aquaculture chromogenic agar
spellingShingle	microdot method sanitary monitoring aquaculture chromogenic agar Strahsburger,Erwin Retamales,Patricio Estrada,Juan Seeger,Michael Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture
description	The microdot method is a downscaling methodology of traditional tenfold serial dilution procedure used in microbiology. The microdot method uses 100 µL for serial dilution and count colonies in a spot of 10 µL. In this study we counted colonies directly in a chromogenic agar plate to determine, at the same time, the presence and cell concentration of target bacteria required for sanitary monitoring of Chilean export fishery products. Due to among importers countries the most concerning bacteria included in sanitary monitoring are Escherichia coli, Listeria monocytogenes and Staphylococcus aureus, we used the chromogenic agar; CHROMagar ECC, CHROMagar Listeria and Baird Parker agar, respectively. The results shows no differences between quantitative results obtained with microdot and traditional method during the quantification of a culture of Escherichia coli (1.5 L). The sensitivity and specificity of the microdot method in association with each chromogenic agar was demonstrated in vitro with reference strains. In addition, the usefulness in sanitary monitoring of aquaculture procedures was evaluated in Artemia salina tanks. This method did not detected sanitary problems in surface water. Although other colonies grown in the chromogenic agar plate, their morphological and chromogenic properties not correspond to Escherichia coli, Listeria monocytogenes and Staphylococcus aureus, being identified as Salmonella enterica subsp. enterica, Microbacterium sp., Bacillus sp. and Staphylococcus pasteuri by 16S rRNA gene sequence analysis. Hence, we propose the microdot chromogenic method as a low cost, specific and reliable procedure for sanitary monitoring of aquaculture procedures.
author	Strahsburger,Erwin Retamales,Patricio Estrada,Juan Seeger,Michael
author_facet	Strahsburger,Erwin Retamales,Patricio Estrada,Juan Seeger,Michael
author_sort	Strahsburger,Erwin
title	Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture
title_short	Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture
title_full	Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture
title_fullStr	Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture
title_full_unstemmed	Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture
title_sort	microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000400009
work_keys_str_mv	AT strahsburgererwin microdotmethodusedwithchromogenicagarisausefulprocedureforsanitarymonitoringinaquaculture AT retamalespatricio microdotmethodusedwithchromogenicagarisausefulprocedureforsanitarymonitoringinaquaculture AT estradajuan microdotmethodusedwithchromogenicagarisausefulprocedureforsanitarymonitoringinaquaculture AT seegermichael microdotmethodusedwithchromogenicagarisausefulprocedureforsanitarymonitoringinaquaculture
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Microdot method: used with chromogenic agar is a useful procedure for sanitary monitoring in aquaculture

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