Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry
As an alternative use of natural marine resources, processing waste from whitemouth croaker was used as raw material for the production of fish protein hydrolysate (FPH). The biometric characterization of the raw material was determined, and the optimal conditions of the protease established using D...
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Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
2016
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oai:scielo:S0718-560X20160005000092016-12-15Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industryAmorim,Ricardo Gaya Oliveira deDeschamps,Francisco CarlosPessatti,Marcos Luiz Micropogonias furnieri fish protein hydrolysate fish waste protease As an alternative use of natural marine resources, processing waste from whitemouth croaker was used as raw material for the production of fish protein hydrolysate (FPH). The biometric characterization of the raw material was determined, and the optimal conditions of the protease established using DH (degree of hydrolysis) as the criterion. Bromatological analyses were performed according to AOAC methods. The DH were determined by the TCA (trichloroacetic acid) method and expressed as a percentage of solubilized protein after hydrolysis compared to the total protein in raw material. The average yields of viscera, heads and carcasses were 7.4, 36.3 and 56.3%, respectively. The optimal conditions for enzyme action on the viscera were: 55°C, hydrolysis time of 90 min, and enzyme concentration of 0.4 mg mL-1. For the muscle, the conditions established were: 45°C, 90 min, and 0.8 mg mL-1. The dry matter of hydrolysate from viscera yielded 73.5% supernatant (soluble fraction) and 27.5% precipitate (insoluble fraction), while that from muscle yielded 45.7% and 54.3%, respectively. The hydrolysate from viscera presented 86.0% proteins, 0.4% lipids and 4.4% ashes in the soluble fraction, and 51.0% proteins, 25.8% lipids and 5.1% ashes in the insoluble fraction. The hydrolysate from muscle presented 86.0% proteins, 0.4% lipids and 9.0% ashes in the soluble fraction, and 87.0% proteins, 4.4% lipids and 2.4% ashes in the insoluble fraction. A lower degree of hydrolysis of hydrolysate from muscle than that produced from viscera was observed, demonstrating the different interactions between the enzyme and the residues studied.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.44 n.5 20162016-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000500009en10.3856/vol44-issue5-fulltext-9 |
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Micropogonias furnieri fish protein hydrolysate fish waste protease |
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Micropogonias furnieri fish protein hydrolysate fish waste protease Amorim,Ricardo Gaya Oliveira de Deschamps,Francisco Carlos Pessatti,Marcos Luiz Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry |
description |
As an alternative use of natural marine resources, processing waste from whitemouth croaker was used as raw material for the production of fish protein hydrolysate (FPH). The biometric characterization of the raw material was determined, and the optimal conditions of the protease established using DH (degree of hydrolysis) as the criterion. Bromatological analyses were performed according to AOAC methods. The DH were determined by the TCA (trichloroacetic acid) method and expressed as a percentage of solubilized protein after hydrolysis compared to the total protein in raw material. The average yields of viscera, heads and carcasses were 7.4, 36.3 and 56.3%, respectively. The optimal conditions for enzyme action on the viscera were: 55°C, hydrolysis time of 90 min, and enzyme concentration of 0.4 mg mL-1. For the muscle, the conditions established were: 45°C, 90 min, and 0.8 mg mL-1. The dry matter of hydrolysate from viscera yielded 73.5% supernatant (soluble fraction) and 27.5% precipitate (insoluble fraction), while that from muscle yielded 45.7% and 54.3%, respectively. The hydrolysate from viscera presented 86.0% proteins, 0.4% lipids and 4.4% ashes in the soluble fraction, and 51.0% proteins, 25.8% lipids and 5.1% ashes in the insoluble fraction. The hydrolysate from muscle presented 86.0% proteins, 0.4% lipids and 9.0% ashes in the soluble fraction, and 87.0% proteins, 4.4% lipids and 2.4% ashes in the insoluble fraction. A lower degree of hydrolysis of hydrolysate from muscle than that produced from viscera was observed, demonstrating the different interactions between the enzyme and the residues studied. |
author |
Amorim,Ricardo Gaya Oliveira de Deschamps,Francisco Carlos Pessatti,Marcos Luiz |
author_facet |
Amorim,Ricardo Gaya Oliveira de Deschamps,Francisco Carlos Pessatti,Marcos Luiz |
author_sort |
Amorim,Ricardo Gaya Oliveira de |
title |
Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry |
title_short |
Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry |
title_full |
Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry |
title_fullStr |
Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry |
title_full_unstemmed |
Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry |
title_sort |
protein hydrolysate waste of whitemouth croaker (micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry |
publisher |
Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar |
publishDate |
2016 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000500009 |
work_keys_str_mv |
AT amorimricardogayaoliveirade proteinhydrolysatewasteofwhitemouthcroakermicropogoniasfurnieriasawayofaddingvaluetofishandreducingtheenvironmentalliabilitiesofthefishingindustry AT deschampsfranciscocarlos proteinhydrolysatewasteofwhitemouthcroakermicropogoniasfurnieriasawayofaddingvaluetofishandreducingtheenvironmentalliabilitiesofthefishingindustry AT pessattimarcosluiz proteinhydrolysatewasteofwhitemouthcroakermicropogoniasfurnieriasawayofaddingvaluetofishandreducingtheenvironmentalliabilitiesofthefishingindustry |
_version_ |
1714205172365787136 |