Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry

As an alternative use of natural marine resources, processing waste from whitemouth croaker was used as raw material for the production of fish protein hydrolysate (FPH). The biometric characterization of the raw material was determined, and the optimal conditions of the protease established using D...

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Autores principales: Amorim,Ricardo Gaya Oliveira de, Deschamps,Francisco Carlos, Pessatti,Marcos Luiz
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2016
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000500009
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spelling oai:scielo:S0718-560X20160005000092016-12-15Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industryAmorim,Ricardo Gaya Oliveira deDeschamps,Francisco CarlosPessatti,Marcos Luiz Micropogonias furnieri fish protein hydrolysate fish waste protease As an alternative use of natural marine resources, processing waste from whitemouth croaker was used as raw material for the production of fish protein hydrolysate (FPH). The biometric characterization of the raw material was determined, and the optimal conditions of the protease established using DH (degree of hydrolysis) as the criterion. Bromatological analyses were performed according to AOAC methods. The DH were determined by the TCA (trichloroacetic acid) method and expressed as a percentage of solubilized protein after hydrolysis compared to the total protein in raw material. The average yields of viscera, heads and carcasses were 7.4, 36.3 and 56.3%, respectively. The optimal conditions for enzyme action on the viscera were: 55°C, hydrolysis time of 90 min, and enzyme concentration of 0.4 mg mL-1. For the muscle, the conditions established were: 45°C, 90 min, and 0.8 mg mL-1. The dry matter of hydrolysate from viscera yielded 73.5% supernatant (soluble fraction) and 27.5% precipitate (insoluble fraction), while that from muscle yielded 45.7% and 54.3%, respectively. The hydrolysate from viscera presented 86.0% proteins, 0.4% lipids and 4.4% ashes in the soluble fraction, and 51.0% proteins, 25.8% lipids and 5.1% ashes in the insoluble fraction. The hydrolysate from muscle presented 86.0% proteins, 0.4% lipids and 9.0% ashes in the soluble fraction, and 87.0% proteins, 4.4% lipids and 2.4% ashes in the insoluble fraction. A lower degree of hydrolysis of hydrolysate from muscle than that produced from viscera was observed, demonstrating the different interactions between the enzyme and the residues studied.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.44 n.5 20162016-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000500009en10.3856/vol44-issue5-fulltext-9
institution Scielo Chile
collection Scielo Chile
language English
topic Micropogonias furnieri
fish protein hydrolysate
fish waste
protease
spellingShingle Micropogonias furnieri
fish protein hydrolysate
fish waste
protease
Amorim,Ricardo Gaya Oliveira de
Deschamps,Francisco Carlos
Pessatti,Marcos Luiz
Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry
description As an alternative use of natural marine resources, processing waste from whitemouth croaker was used as raw material for the production of fish protein hydrolysate (FPH). The biometric characterization of the raw material was determined, and the optimal conditions of the protease established using DH (degree of hydrolysis) as the criterion. Bromatological analyses were performed according to AOAC methods. The DH were determined by the TCA (trichloroacetic acid) method and expressed as a percentage of solubilized protein after hydrolysis compared to the total protein in raw material. The average yields of viscera, heads and carcasses were 7.4, 36.3 and 56.3%, respectively. The optimal conditions for enzyme action on the viscera were: 55°C, hydrolysis time of 90 min, and enzyme concentration of 0.4 mg mL-1. For the muscle, the conditions established were: 45°C, 90 min, and 0.8 mg mL-1. The dry matter of hydrolysate from viscera yielded 73.5% supernatant (soluble fraction) and 27.5% precipitate (insoluble fraction), while that from muscle yielded 45.7% and 54.3%, respectively. The hydrolysate from viscera presented 86.0% proteins, 0.4% lipids and 4.4% ashes in the soluble fraction, and 51.0% proteins, 25.8% lipids and 5.1% ashes in the insoluble fraction. The hydrolysate from muscle presented 86.0% proteins, 0.4% lipids and 9.0% ashes in the soluble fraction, and 87.0% proteins, 4.4% lipids and 2.4% ashes in the insoluble fraction. A lower degree of hydrolysis of hydrolysate from muscle than that produced from viscera was observed, demonstrating the different interactions between the enzyme and the residues studied.
author Amorim,Ricardo Gaya Oliveira de
Deschamps,Francisco Carlos
Pessatti,Marcos Luiz
author_facet Amorim,Ricardo Gaya Oliveira de
Deschamps,Francisco Carlos
Pessatti,Marcos Luiz
author_sort Amorim,Ricardo Gaya Oliveira de
title Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry
title_short Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry
title_full Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry
title_fullStr Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry
title_full_unstemmed Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry
title_sort protein hydrolysate waste of whitemouth croaker (micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry
publisher Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2016000500009
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