Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish

The Patagonian toothfish or Chilean sea bass (Dissostichus eleginoides), found in the Southern Ocean surrounding Antarctica, is an important fishery species for Chile. This high-value species is regarded as overfished, making it an attractive target for aquaculture. When developing a reproduction pr...

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Autores principales:	Araneda,Cristian, Lam,Natalia, Iturra,Patricia, Jilberto,Felipe, Cordova,Valentina, Gallardo,Pablo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2017
Materias:	Dissostichus eleginoides Chilean sea bass heterozygosity microsatellites genetic diversity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000100018
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spelling	oai:scielo:S0718-560X20170001000182017-04-21Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfishAraneda,CristianLam,NataliaIturra,PatriciaJilberto,FelipeCordova,ValentinaGallardo,Pablo Dissostichus eleginoides Chilean sea bass heterozygosity microsatellites genetic diversity The Patagonian toothfish or Chilean sea bass (Dissostichus eleginoides), found in the Southern Ocean surrounding Antarctica, is an important fishery species for Chile. This high-value species is regarded as overfished, making it an attractive target for aquaculture. When developing a reproduction program for any aquaculture species, it is important to implement genetic tools to evaluate diversity, inbreeding, and parentage. We calculated genetic diversity and paternity/maternity exclusion probabilities based on five commonly-used microsatellite loci in a natural population of Patagonian toothfish from southern Chile (n = 34) in order to evaluate the potential utility of these five markers in stock management. The observed number of alleles per locus (Na) and observed heterozygosities (H O) are within range as described by studies performed in other sub-Antarctic regions. All five loci were strongly polymorphic, with H O > 0.6 and Na > 7, and paternity exclusion probabilities were high (PE > 0.99), indicating the potential utility of these loci in paternity/maternity exclusion analyses of Patagonian toothfish.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.45 n.1 20172017-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000100018en10.3856/vol45-issue1-fulltext-18
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Dissostichus eleginoides Chilean sea bass heterozygosity microsatellites genetic diversity
spellingShingle	Dissostichus eleginoides Chilean sea bass heterozygosity microsatellites genetic diversity Araneda,Cristian Lam,Natalia Iturra,Patricia Jilberto,Felipe Cordova,Valentina Gallardo,Pablo Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish
description	The Patagonian toothfish or Chilean sea bass (Dissostichus eleginoides), found in the Southern Ocean surrounding Antarctica, is an important fishery species for Chile. This high-value species is regarded as overfished, making it an attractive target for aquaculture. When developing a reproduction program for any aquaculture species, it is important to implement genetic tools to evaluate diversity, inbreeding, and parentage. We calculated genetic diversity and paternity/maternity exclusion probabilities based on five commonly-used microsatellite loci in a natural population of Patagonian toothfish from southern Chile (n = 34) in order to evaluate the potential utility of these five markers in stock management. The observed number of alleles per locus (Na) and observed heterozygosities (H O) are within range as described by studies performed in other sub-Antarctic regions. All five loci were strongly polymorphic, with H O > 0.6 and Na > 7, and paternity exclusion probabilities were high (PE > 0.99), indicating the potential utility of these loci in paternity/maternity exclusion analyses of Patagonian toothfish.
author	Araneda,Cristian Lam,Natalia Iturra,Patricia Jilberto,Felipe Cordova,Valentina Gallardo,Pablo
author_facet	Araneda,Cristian Lam,Natalia Iturra,Patricia Jilberto,Felipe Cordova,Valentina Gallardo,Pablo
author_sort	Araneda,Cristian
title	Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish
title_short	Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish
title_full	Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish
title_fullStr	Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish
title_full_unstemmed	Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish
title_sort	utility of five ssr markers for genetic diversity and paternity exclusion analysis in the patagonian toothfish
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000100018
work_keys_str_mv	AT aranedacristian utilityoffivessrmarkersforgeneticdiversityandpaternityexclusionanalysisinthepatagoniantoothfish AT lamnatalia utilityoffivessrmarkersforgeneticdiversityandpaternityexclusionanalysisinthepatagoniantoothfish AT iturrapatricia utilityoffivessrmarkersforgeneticdiversityandpaternityexclusionanalysisinthepatagoniantoothfish AT jilbertofelipe utilityoffivessrmarkersforgeneticdiversityandpaternityexclusionanalysisinthepatagoniantoothfish AT cordovavalentina utilityoffivessrmarkersforgeneticdiversityandpaternityexclusionanalysisinthepatagoniantoothfish AT gallardopablo utilityoffivessrmarkersforgeneticdiversityandpaternityexclusionanalysisinthepatagoniantoothfish
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Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the Patagonian toothfish

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