Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock
This study evaluated sequential double-temperature shock (heat and cold) to induce triploidy in Rhamdia quelen. Fertilized eggs were heat shocked at 37, 39 and 41°C at 2 min post-fertilization and then cold shocked at 1°C for 20 min. Intact eggs were used as controls. Egg viability in control groups...
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Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
2017
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oai:scielo:S0718-560X20170001000222017-04-21Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shockGarcía,SilvanoYasui,George SBernardes-Júnior,Jurandir JCorrêa da Silva,BrunoAmaral-Júnior,HiltonZaniboni-Filho,Evoy Rhamdia quelen silver catfish polyploidy temperature shock flow cytometry This study evaluated sequential double-temperature shock (heat and cold) to induce triploidy in Rhamdia quelen. Fertilized eggs were heat shocked at 37, 39 and 41°C at 2 min post-fertilization and then cold shocked at 1°C for 20 min. Intact eggs were used as controls. Egg viability in control groups was higher (65.5 ± 37.0%) than that at 37°C (58.2 ± 37.7%), 39°C (1.8 ± 0.3%) and 41°C (0%). The survival of control hatchery (64.6 ± 36.8%) was higher compared to treatments at 37°C (24.4 ± 15.5%) and 39°C (0.6 ± 0.07%). Triploid percentages were 98.5% at 37°C and 100% at 39°C. Therefore, while double-temperature shock induced 100% triploidy in R. quelen, it also significantly decreased embryo survival and increased the deformity larvae.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.45 n.1 20172017-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000100022en10.3856/vol45-issue1-fulltext-22 |
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Scielo Chile |
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Scielo Chile |
language |
English |
topic |
Rhamdia quelen silver catfish polyploidy temperature shock flow cytometry |
spellingShingle |
Rhamdia quelen silver catfish polyploidy temperature shock flow cytometry García,Silvano Yasui,George S Bernardes-Júnior,Jurandir J Corrêa da Silva,Bruno Amaral-Júnior,Hilton Zaniboni-Filho,Evoy Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock |
description |
This study evaluated sequential double-temperature shock (heat and cold) to induce triploidy in Rhamdia quelen. Fertilized eggs were heat shocked at 37, 39 and 41°C at 2 min post-fertilization and then cold shocked at 1°C for 20 min. Intact eggs were used as controls. Egg viability in control groups was higher (65.5 ± 37.0%) than that at 37°C (58.2 ± 37.7%), 39°C (1.8 ± 0.3%) and 41°C (0%). The survival of control hatchery (64.6 ± 36.8%) was higher compared to treatments at 37°C (24.4 ± 15.5%) and 39°C (0.6 ± 0.07%). Triploid percentages were 98.5% at 37°C and 100% at 39°C. Therefore, while double-temperature shock induced 100% triploidy in R. quelen, it also significantly decreased embryo survival and increased the deformity larvae. |
author |
García,Silvano Yasui,George S Bernardes-Júnior,Jurandir J Corrêa da Silva,Bruno Amaral-Júnior,Hilton Zaniboni-Filho,Evoy |
author_facet |
García,Silvano Yasui,George S Bernardes-Júnior,Jurandir J Corrêa da Silva,Bruno Amaral-Júnior,Hilton Zaniboni-Filho,Evoy |
author_sort |
García,Silvano |
title |
Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock |
title_short |
Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock |
title_full |
Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock |
title_fullStr |
Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock |
title_full_unstemmed |
Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock |
title_sort |
induction of triploidy in rhamdia quelen (siluriformes, heptapteridae) by double-temperature shock |
publisher |
Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar |
publishDate |
2017 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000100022 |
work_keys_str_mv |
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