Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings

ABSTRACT Variable individual growth rate is a phenomenon observed in fish cohorts that influences the aquaculture performance and fish cohort ecological viability. Our aim was to compare gene expression patterns of key growth genes in zebrafish larvae with different growth rate. The body length of s...

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Autores principales:	Opazo,Rafael, Valladares,Luis, Romero,Jaime
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2017
Materias:	Danio rerio zebrafish growth rate IGF-1R IGFBP-1 aquaculture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000400766
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spelling	oai:scielo:S0718-560X20170004007662017-10-11Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblingsOpazo,RafaelValladares,LuisRomero,Jaime Danio rerio zebrafish growth rate IGF-1R IGFBP-1 aquaculture ABSTRACT Variable individual growth rate is a phenomenon observed in fish cohorts that influences the aquaculture performance and fish cohort ecological viability. Our aim was to compare gene expression patterns of key growth genes in zebrafish larvae with different growth rate. The body length of sibling zebrafish larvae at 6 days post hatching (dph) was measured. The larvae were reared to 20 dph and measured again. Two body-length groups were clearly observed: 4 mm (small larvae) and 5-6 mm (large larvae). Total RNA was isolated from both groups. Growth hormone (gh), growth hormone receptor (ghr), insulin-like growth factor 1 (igf-1), insulin-like growth factor receptor (igf-1r a/b), insulin-like growth factor binding protein 1 (igfbp-1), thyroglobulin (tg), cholecystokinin (cck), and ghrelin were evaluated by quantitative polymerase chain reaction (qPCR). Glucokinase (gck) and igfbp-1 were included as a gene expression marker of larvae nutritional status. Two genes showed significant differences between the body length groups, igfbp-1 (P = 0.01) and igf-1r (P = 0.02). The igfbp-1 suggests than growth rate variability was associated with the larvae nutritional status and this condition affect the gene expression pattern of igf-1r. Therefore these genes are interesting genes markers for growth rate variabilities studies.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.45 n.4 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000400766en10.3856/vol45-issue4-fulltext-12
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Danio rerio zebrafish growth rate IGF-1R IGFBP-1 aquaculture
spellingShingle	Danio rerio zebrafish growth rate IGF-1R IGFBP-1 aquaculture Opazo,Rafael Valladares,Luis Romero,Jaime Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings
description	ABSTRACT Variable individual growth rate is a phenomenon observed in fish cohorts that influences the aquaculture performance and fish cohort ecological viability. Our aim was to compare gene expression patterns of key growth genes in zebrafish larvae with different growth rate. The body length of sibling zebrafish larvae at 6 days post hatching (dph) was measured. The larvae were reared to 20 dph and measured again. Two body-length groups were clearly observed: 4 mm (small larvae) and 5-6 mm (large larvae). Total RNA was isolated from both groups. Growth hormone (gh), growth hormone receptor (ghr), insulin-like growth factor 1 (igf-1), insulin-like growth factor receptor (igf-1r a/b), insulin-like growth factor binding protein 1 (igfbp-1), thyroglobulin (tg), cholecystokinin (cck), and ghrelin were evaluated by quantitative polymerase chain reaction (qPCR). Glucokinase (gck) and igfbp-1 were included as a gene expression marker of larvae nutritional status. Two genes showed significant differences between the body length groups, igfbp-1 (P = 0.01) and igf-1r (P = 0.02). The igfbp-1 suggests than growth rate variability was associated with the larvae nutritional status and this condition affect the gene expression pattern of igf-1r. Therefore these genes are interesting genes markers for growth rate variabilities studies.
author	Opazo,Rafael Valladares,Luis Romero,Jaime
author_facet	Opazo,Rafael Valladares,Luis Romero,Jaime
author_sort	Opazo,Rafael
title	Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings
title_short	Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings
title_full	Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings
title_fullStr	Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings
title_full_unstemmed	Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings
title_sort	comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (danio rerio) siblings
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000400766
work_keys_str_mv	AT opazorafael comparisonofgeneexpressionpatternsofkeygrowthgenesbetweendifferentrategrowthsinzebrafishdanioreriosiblings AT valladaresluis comparisonofgeneexpressionpatternsofkeygrowthgenesbetweendifferentrategrowthsinzebrafishdanioreriosiblings AT romerojaime comparisonofgeneexpressionpatternsofkeygrowthgenesbetweendifferentrategrowthsinzebrafishdanioreriosiblings
_version_	1714205186512125952

Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings

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