Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus
ABSTRACT Circulating levels of estradiol, vitellogenin and prostaglandins could be references of oocytes quality in the aquaculture industry. This study evaluated the performance of 17β-estradiol (E2) and vitellogenin (Vtg) levels in plasma and prostaglandins in oocytes (PGE 2) during the r...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
2017
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oai:scielo:S0718-560X20170005009302017-12-07Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticusOrtiz Tirado,JuanValladares,LuisMuñoz,DaysiCaza,JairoManjunatha,BangeppagariR. Kundapur,Rajesh Oreochromis niloticus tilapia estradiol vitellogenin pituitary prostaglandin E2 ABSTRACT Circulating levels of estradiol, vitellogenin and prostaglandins could be references of oocytes quality in the aquaculture industry. This study evaluated the performance of 17β-estradiol (E2) and vitellogenin (Vtg) levels in plasma and prostaglandins in oocytes (PGE 2) during the reproductive period of Oreochromis niloticus in a commercial farm in Ecuador. Adults fish females were treated with estradiol-synthetic (ES) and tilapia pituitary extracts (TP) during 25 days. For quantification of E2 and PGE 2, an enzyme-linked immunosorbent assay (ELISA) was used, while for Vtg relative quantification a Western blot analysis was performed. Circulating E2 concentrations were different during the first week of follow-up (P < 0.05) resulting in different levels of Vtg per treatment (P < 0.05) affecting on gonadal growth. The ovaries had an asynchronous development, where the mature oocytes proportions were higher with ES and TP than control. Relative fecundity was different between treatments (P < 0.05), where TP generated 15.7 ± 5.8 oocytes per g of spawning female. Furthermore, concentrations of PGE 2 were different between treatments (P < 0.05). The information reported in this study will be useful to improve the reproduction methods and have some biological signals before ovulation in tilapia commercial culture.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.45 n.5 20172017-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000500930en10.3856/vol45-issue5-fulltext-8 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Oreochromis niloticus tilapia estradiol vitellogenin pituitary prostaglandin E2 |
| spellingShingle |
Oreochromis niloticus tilapia estradiol vitellogenin pituitary prostaglandin E2 Ortiz Tirado,Juan Valladares,Luis Muñoz,Daysi Caza,Jairo Manjunatha,Bangeppagari R. Kundapur,Rajesh Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus |
| description |
ABSTRACT Circulating levels of estradiol, vitellogenin and prostaglandins could be references of oocytes quality in the aquaculture industry. This study evaluated the performance of 17β-estradiol (E2) and vitellogenin (Vtg) levels in plasma and prostaglandins in oocytes (PGE 2) during the reproductive period of Oreochromis niloticus in a commercial farm in Ecuador. Adults fish females were treated with estradiol-synthetic (ES) and tilapia pituitary extracts (TP) during 25 days. For quantification of E2 and PGE 2, an enzyme-linked immunosorbent assay (ELISA) was used, while for Vtg relative quantification a Western blot analysis was performed. Circulating E2 concentrations were different during the first week of follow-up (P < 0.05) resulting in different levels of Vtg per treatment (P < 0.05) affecting on gonadal growth. The ovaries had an asynchronous development, where the mature oocytes proportions were higher with ES and TP than control. Relative fecundity was different between treatments (P < 0.05), where TP generated 15.7 ± 5.8 oocytes per g of spawning female. Furthermore, concentrations of PGE 2 were different between treatments (P < 0.05). The information reported in this study will be useful to improve the reproduction methods and have some biological signals before ovulation in tilapia commercial culture. |
| author |
Ortiz Tirado,Juan Valladares,Luis Muñoz,Daysi Caza,Jairo Manjunatha,Bangeppagari R. Kundapur,Rajesh |
| author_facet |
Ortiz Tirado,Juan Valladares,Luis Muñoz,Daysi Caza,Jairo Manjunatha,Bangeppagari R. Kundapur,Rajesh |
| author_sort |
Ortiz Tirado,Juan |
| title |
Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus |
| title_short |
Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus |
| title_full |
Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus |
| title_fullStr |
Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus |
| title_full_unstemmed |
Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus |
| title_sort |
levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of oreochromis niloticus |
| publisher |
Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar |
| publishDate |
2017 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000500930 |
| work_keys_str_mv |
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| _version_ |
1714205190226182144 |