Proteomic characterization of vitellogenins from three species of South American fresh water fish

ABSTRACT Vitellogenins (Vtg) are glycolipophosphoproteins synthesized by oviparous vertebrates as yolk proteins precursors. These proteins have been studied for their role in reproduction and in endocrine disruption. In this work, we report the first proteomic study towards the characterization of V...

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Autores principales:	Urdaneta,Verónica, Camafeita,Emilio, Poleo,Germán, Guerrero,Hilda, Bernal,Carolina, Galindo-Castro,Ivan, Diez,Nardy
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2018
Materias:	Pseudoplatystoma fasciatum Piaractus brachypomus Colossoma macropomum vitellogenin proteomics mass spectrometry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000100187
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spelling	oai:scielo:S0718-560X20180001001872018-04-17Proteomic characterization of vitellogenins from three species of South American fresh water fishUrdaneta,VerónicaCamafeita,EmilioPoleo,GermánGuerrero,HildaBernal,CarolinaGalindo-Castro,IvanDiez,Nardy Pseudoplatystoma fasciatum Piaractus brachypomus Colossoma macropomum vitellogenin proteomics mass spectrometry ABSTRACT Vitellogenins (Vtg) are glycolipophosphoproteins synthesized by oviparous vertebrates as yolk proteins precursors. These proteins have been studied for their role in reproduction and in endocrine disruption. In this work, we report the first proteomic study towards the characterization of Vtg from Pseudoplatystoma fasciatum, Piaractus brachypomus and Colossoma macropomum. Male specimens of each of the three fish species were estradiol-induced (experimental) and non-induced (control). The initial Vtg characterization was made by 2D protein gel electrophoresis of both groups The identification of the high molecular weight spots, presumed to be Vtgs, was assessed by MALDI-TOF mass spectrometry analysis. A post-translational modification study was performed by differential staining of 2D gels in order to visualize phosphoproteins and glycoproteins. Plasma samples from the three species, induced with estrogen, showed three high molecular weight spots with variable isoelectric points. Post-translational modifications showed that Vtgs from P. brachypomus and C. macropomum presented a phosphorylated and glycosylated subunit, while the same subunit in P. fasciatum was only glycosylated. This characterization will help in the development of reliable immunoassays, which could be used for studies of endocrine disruption or for the improvement of artificial spawning, by uncovering the time of fish maturation or sex determination.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.46 n.1 20182018-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000100187en10.3856/vol46-issue1-fulltext-18
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pseudoplatystoma fasciatum Piaractus brachypomus Colossoma macropomum vitellogenin proteomics mass spectrometry
spellingShingle	Pseudoplatystoma fasciatum Piaractus brachypomus Colossoma macropomum vitellogenin proteomics mass spectrometry Urdaneta,Verónica Camafeita,Emilio Poleo,Germán Guerrero,Hilda Bernal,Carolina Galindo-Castro,Ivan Diez,Nardy Proteomic characterization of vitellogenins from three species of South American fresh water fish
description	ABSTRACT Vitellogenins (Vtg) are glycolipophosphoproteins synthesized by oviparous vertebrates as yolk proteins precursors. These proteins have been studied for their role in reproduction and in endocrine disruption. In this work, we report the first proteomic study towards the characterization of Vtg from Pseudoplatystoma fasciatum, Piaractus brachypomus and Colossoma macropomum. Male specimens of each of the three fish species were estradiol-induced (experimental) and non-induced (control). The initial Vtg characterization was made by 2D protein gel electrophoresis of both groups The identification of the high molecular weight spots, presumed to be Vtgs, was assessed by MALDI-TOF mass spectrometry analysis. A post-translational modification study was performed by differential staining of 2D gels in order to visualize phosphoproteins and glycoproteins. Plasma samples from the three species, induced with estrogen, showed three high molecular weight spots with variable isoelectric points. Post-translational modifications showed that Vtgs from P. brachypomus and C. macropomum presented a phosphorylated and glycosylated subunit, while the same subunit in P. fasciatum was only glycosylated. This characterization will help in the development of reliable immunoassays, which could be used for studies of endocrine disruption or for the improvement of artificial spawning, by uncovering the time of fish maturation or sex determination.
author	Urdaneta,Verónica Camafeita,Emilio Poleo,Germán Guerrero,Hilda Bernal,Carolina Galindo-Castro,Ivan Diez,Nardy
author_facet	Urdaneta,Verónica Camafeita,Emilio Poleo,Germán Guerrero,Hilda Bernal,Carolina Galindo-Castro,Ivan Diez,Nardy
author_sort	Urdaneta,Verónica
title	Proteomic characterization of vitellogenins from three species of South American fresh water fish
title_short	Proteomic characterization of vitellogenins from three species of South American fresh water fish
title_full	Proteomic characterization of vitellogenins from three species of South American fresh water fish
title_fullStr	Proteomic characterization of vitellogenins from three species of South American fresh water fish
title_full_unstemmed	Proteomic characterization of vitellogenins from three species of South American fresh water fish
title_sort	proteomic characterization of vitellogenins from three species of south american fresh water fish
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000100187
work_keys_str_mv	AT urdanetaveronica proteomiccharacterizationofvitellogeninsfromthreespeciesofsouthamericanfreshwaterfish AT camafeitaemilio proteomiccharacterizationofvitellogeninsfromthreespeciesofsouthamericanfreshwaterfish AT poleogerman proteomiccharacterizationofvitellogeninsfromthreespeciesofsouthamericanfreshwaterfish AT guerrerohilda proteomiccharacterizationofvitellogeninsfromthreespeciesofsouthamericanfreshwaterfish AT bernalcarolina proteomiccharacterizationofvitellogeninsfromthreespeciesofsouthamericanfreshwaterfish AT galindocastroivan proteomiccharacterizationofvitellogeninsfromthreespeciesofsouthamericanfreshwaterfish AT dieznardy proteomiccharacterizationofvitellogeninsfromthreespeciesofsouthamericanfreshwaterfish
_version_	1714205196052070400

Proteomic characterization of vitellogenins from three species of South American fresh water fish

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