Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)

ABSTRACT The objectives of the present study were to characterize biochemically the proteases present in gastric juices from adults of the southern king crab (Lithodes santolla) and the Chilean rock crab (Cancer edwardsii), to determine the classes of proteases using specific inhibitors, as well as...

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Autores principales:	Bañuelos-Vargas,Isaura, Cárdenas-Chávez,Fredy, Paschke,Kurt, Román-Reyes,José Cristóbal, Salazar-Leyva,Jesús Aarón, Martínez-Montaño,Emmanuel
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2018
Materias:	Lithodes santolla Cancer edwardsii digestive physiology proteases protein inhibitors subpolar crabs
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200289
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spelling	oai:scielo:S0718-560X20180002002892018-06-13Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)Bañuelos-Vargas,IsauraCárdenas-Chávez,FredyPaschke,KurtRomán-Reyes,José CristóbalSalazar-Leyva,Jesús AarónMartínez-Montaño,Emmanuel Lithodes santolla Cancer edwardsii digestive physiology proteases protein inhibitors subpolar crabs ABSTRACT The objectives of the present study were to characterize biochemically the proteases present in gastric juices from adults of the southern king crab (Lithodes santolla) and the Chilean rock crab (Cancer edwardsii), to determine the classes of proteases using specific inhibitors, as well as to evaluate the effect of temperature and pH that affect the proteolytic activity. The enzyme activity analysis revealed non-significant differences between the activities of serine proteases (trypsin and chymotrypsin) in both species. On the other hand, acid protease activity in C. edwardsii was significantly higher than in L. santolla. The activity of aspartate proteases was inhibited by Pepstatin A in both species, with no significant differences in the extent of inhibition between them. The maximal enzyme activity detected for both species occurred at alkaline pH. The optimum pH for proteolytic activity was ranged between 7 and 9 for C. edwardsii, and between 7 and 11 for L. santolla. Moreover, the highest proteolytic activity was recorded at 60°C in both species. However, at this temperature, enzyme activity was unstable after 20 min. Finally, collagenase-like activity in the gastric juices was detected for both species; this activity deserves further investigation, considering its biotechnological potential.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.46 n.2 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200289en10.3856/vol46-issue2-fulltext-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lithodes santolla Cancer edwardsii digestive physiology proteases protein inhibitors subpolar crabs
spellingShingle	Lithodes santolla Cancer edwardsii digestive physiology proteases protein inhibitors subpolar crabs Bañuelos-Vargas,Isaura Cárdenas-Chávez,Fredy Paschke,Kurt Román-Reyes,José Cristóbal Salazar-Leyva,Jesús Aarón Martínez-Montaño,Emmanuel Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)
description	ABSTRACT The objectives of the present study were to characterize biochemically the proteases present in gastric juices from adults of the southern king crab (Lithodes santolla) and the Chilean rock crab (Cancer edwardsii), to determine the classes of proteases using specific inhibitors, as well as to evaluate the effect of temperature and pH that affect the proteolytic activity. The enzyme activity analysis revealed non-significant differences between the activities of serine proteases (trypsin and chymotrypsin) in both species. On the other hand, acid protease activity in C. edwardsii was significantly higher than in L. santolla. The activity of aspartate proteases was inhibited by Pepstatin A in both species, with no significant differences in the extent of inhibition between them. The maximal enzyme activity detected for both species occurred at alkaline pH. The optimum pH for proteolytic activity was ranged between 7 and 9 for C. edwardsii, and between 7 and 11 for L. santolla. Moreover, the highest proteolytic activity was recorded at 60°C in both species. However, at this temperature, enzyme activity was unstable after 20 min. Finally, collagenase-like activity in the gastric juices was detected for both species; this activity deserves further investigation, considering its biotechnological potential.
author	Bañuelos-Vargas,Isaura Cárdenas-Chávez,Fredy Paschke,Kurt Román-Reyes,José Cristóbal Salazar-Leyva,Jesús Aarón Martínez-Montaño,Emmanuel
author_facet	Bañuelos-Vargas,Isaura Cárdenas-Chávez,Fredy Paschke,Kurt Román-Reyes,José Cristóbal Salazar-Leyva,Jesús Aarón Martínez-Montaño,Emmanuel
author_sort	Bañuelos-Vargas,Isaura
title	Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)
title_short	Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)
title_full	Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)
title_fullStr	Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)
title_full_unstemmed	Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)
title_sort	partial biochemical characterization of digestive proteases present in the gastric juices of two chilean crustaceans, lithodes santolla (molina, 1782) and cancer edwardsii (bell, 1835)
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200289
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Partial biochemical characterization of digestive proteases present in the gastric juices of two Chilean crustaceans, Lithodes santolla (Molina, 1782) and Cancer edwardsii (Bell, 1835)

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