Denitrifying community structure variability in the Colombian Pacific

ABSTRACT Denitrifying communities were detected in the water column of the Colombian Pacific Basin, with oxygen levels between 6 and 56 μΜ. They were analyzed by Terminal Restriction Fragment Length Polymorphism (TRFLP) of functional genes nitrite reductase (nirS) and nitrous oxide...

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Autores principales:	Castro-González,Maribeb, Rodríguez-Rubio,Efraín, Castro,Angela
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2018
Materias:	denitrifying communities functional genes Oxygen Minimum Zones Colombian Pacific
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200392
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spelling	oai:scielo:S0718-560X20180002003922018-06-13Denitrifying community structure variability in the Colombian PacificCastro-González,MaribebRodríguez-Rubio,EfraínCastro,Angela denitrifying communities functional genes Oxygen Minimum Zones Colombian Pacific ABSTRACT Denitrifying communities were detected in the water column of the Colombian Pacific Basin, with oxygen levels between 6 and 56 μΜ. They were analyzed by Terminal Restriction Fragment Length Polymorphism (TRFLP) of functional genes nitrite reductase (nirS) and nitrous oxide reductase (nosZ), and by pyrosequencing the gene nosZ. These genes are responsible for the production and consumption of the greenhouse gas N2O. The TRFLP analysis indicated that throughout the Colombia current, the composition of the nirS-type denitrifying community was more homogeneous than the nosZ-type community. The nosZ-type community presented the highest richness (up to 11 TRF) and diversity (Simpson Index 1/D = 4.85 and Shannon Index = 0.76) of terminal restriction fragments, as well as the higher richness of operational taxonomic units-OTUs (up to 71), obtained via pyrosequencing. Out of 84% of OTUs, 48% were assigned to the genus Pseudomonas, and 36% to environmental clones. These results indicate the presence of a nirS-community inhabiting the Colombian Pacific Basin, with low diversity and mostly restricted within 300 m depth. The nosZ- community showed similar richness and diversity of TRFs and OTUs to that reported for the South Pacific Ocean. These results demonstrate that although both denitrifying communities are present in the area, these communities differ in structure and dominance compared to previous reports for other denitrifying microorganisms.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.46 n.2 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200392en10.3856/vol46-issue2-fulltext-15
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	denitrifying communities functional genes Oxygen Minimum Zones Colombian Pacific
spellingShingle	denitrifying communities functional genes Oxygen Minimum Zones Colombian Pacific Castro-González,Maribeb Rodríguez-Rubio,Efraín Castro,Angela Denitrifying community structure variability in the Colombian Pacific
description	ABSTRACT Denitrifying communities were detected in the water column of the Colombian Pacific Basin, with oxygen levels between 6 and 56 μΜ. They were analyzed by Terminal Restriction Fragment Length Polymorphism (TRFLP) of functional genes nitrite reductase (nirS) and nitrous oxide reductase (nosZ), and by pyrosequencing the gene nosZ. These genes are responsible for the production and consumption of the greenhouse gas N2O. The TRFLP analysis indicated that throughout the Colombia current, the composition of the nirS-type denitrifying community was more homogeneous than the nosZ-type community. The nosZ-type community presented the highest richness (up to 11 TRF) and diversity (Simpson Index 1/D = 4.85 and Shannon Index = 0.76) of terminal restriction fragments, as well as the higher richness of operational taxonomic units-OTUs (up to 71), obtained via pyrosequencing. Out of 84% of OTUs, 48% were assigned to the genus Pseudomonas, and 36% to environmental clones. These results indicate the presence of a nirS-community inhabiting the Colombian Pacific Basin, with low diversity and mostly restricted within 300 m depth. The nosZ- community showed similar richness and diversity of TRFs and OTUs to that reported for the South Pacific Ocean. These results demonstrate that although both denitrifying communities are present in the area, these communities differ in structure and dominance compared to previous reports for other denitrifying microorganisms.
author	Castro-González,Maribeb Rodríguez-Rubio,Efraín Castro,Angela
author_facet	Castro-González,Maribeb Rodríguez-Rubio,Efraín Castro,Angela
author_sort	Castro-González,Maribeb
title	Denitrifying community structure variability in the Colombian Pacific
title_short	Denitrifying community structure variability in the Colombian Pacific
title_full	Denitrifying community structure variability in the Colombian Pacific
title_fullStr	Denitrifying community structure variability in the Colombian Pacific
title_full_unstemmed	Denitrifying community structure variability in the Colombian Pacific
title_sort	denitrifying community structure variability in the colombian pacific
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200392
work_keys_str_mv	AT castrogonzalezmaribeb denitrifyingcommunitystructurevariabilityinthecolombianpacific AT rodriguezrubioefrain denitrifyingcommunitystructurevariabilityinthecolombianpacific AT castroangela denitrifyingcommunitystructurevariabilityinthecolombianpacific
_version_	1714205199705309184

Denitrifying community structure variability in the Colombian Pacific

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