Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencing

ABSTRACT The anchovy, Engraulis ringens, is an economically valuable fish, and the most heavily exploited resource in the Humboldt Current System. To analyze its genetic structure and diversity, microsatellite markers were developed using 454 pyrosequencing. A total of 27,352 reads containing di-, t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ferrada-Fuentes,Sandra, Galleguillos,Ricardo, Canales-Aguirre,Cristian B., Herrera-Yañez,Victoria
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2018
Materias:	Engraulis ringens Peruvian anchovy genetic diversity Humboldt Current System Chile
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200452
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-560X2018000200452
record_format	dspace
spelling	oai:scielo:S0718-560X20180002004522018-06-13Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencingFerrada-Fuentes,SandraGalleguillos,RicardoCanales-Aguirre,Cristian B.Herrera-Yañez,Victoria Engraulis ringens Peruvian anchovy genetic diversity Humboldt Current System Chile ABSTRACT The anchovy, Engraulis ringens, is an economically valuable fish, and the most heavily exploited resource in the Humboldt Current System. To analyze its genetic structure and diversity, microsatellite markers were developed using 454 pyrosequencing. A total of 27,352 reads containing di-, tri-, tetra-, penta-, and hexanucleotide microsatellite repeat units were identified from 136,537 reads. Among 80 loci containing more than six repeat motifs, 32 primer sets (40%) produced reproducible PCR products, and all of these loci were polymorphic. Some loci showed deviations from HWE and possible null allele's presence, results of an excess of homozygotes. In an analysis of 45 individuals from one E. ringens population, the number of alleles per locus ranged from 2 to 33, observed heterozygosity ranged from 0.171 to 0.976, and the probability of identity values ranged from 0.006 to 0.513. These microsatellites will be useful for numerous ecological studies focused on this important pelagic fish; including the examination of population genetic structure, estimating effective population size and providing information for fisheries management.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.46 n.2 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200452en10.3856/vol46-issue2-fulltext-19
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Engraulis ringens Peruvian anchovy genetic diversity Humboldt Current System Chile
spellingShingle	Engraulis ringens Peruvian anchovy genetic diversity Humboldt Current System Chile Ferrada-Fuentes,Sandra Galleguillos,Ricardo Canales-Aguirre,Cristian B. Herrera-Yañez,Victoria Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencing
description	ABSTRACT The anchovy, Engraulis ringens, is an economically valuable fish, and the most heavily exploited resource in the Humboldt Current System. To analyze its genetic structure and diversity, microsatellite markers were developed using 454 pyrosequencing. A total of 27,352 reads containing di-, tri-, tetra-, penta-, and hexanucleotide microsatellite repeat units were identified from 136,537 reads. Among 80 loci containing more than six repeat motifs, 32 primer sets (40%) produced reproducible PCR products, and all of these loci were polymorphic. Some loci showed deviations from HWE and possible null allele's presence, results of an excess of homozygotes. In an analysis of 45 individuals from one E. ringens population, the number of alleles per locus ranged from 2 to 33, observed heterozygosity ranged from 0.171 to 0.976, and the probability of identity values ranged from 0.006 to 0.513. These microsatellites will be useful for numerous ecological studies focused on this important pelagic fish; including the examination of population genetic structure, estimating effective population size and providing information for fisheries management.
author	Ferrada-Fuentes,Sandra Galleguillos,Ricardo Canales-Aguirre,Cristian B. Herrera-Yañez,Victoria
author_facet	Ferrada-Fuentes,Sandra Galleguillos,Ricardo Canales-Aguirre,Cristian B. Herrera-Yañez,Victoria
author_sort	Ferrada-Fuentes,Sandra
title	Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencing
title_short	Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencing
title_full	Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencing
title_fullStr	Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencing
title_full_unstemmed	Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencing
title_sort	development and characterization of thirty-two microsatellite markers for the anchovy, engraulis ringens jenyns, 1842 (clupeiformes, engraulidae) via 454 pyrosequencing
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200452
work_keys_str_mv	AT ferradafuentessandra developmentandcharacterizationofthirtytwomicrosatellitemarkersfortheanchovyengraulisringensjenyns1842clupeiformesengraulidaevia454pyrosequencing AT galleguillosricardo developmentandcharacterizationofthirtytwomicrosatellitemarkersfortheanchovyengraulisringensjenyns1842clupeiformesengraulidaevia454pyrosequencing AT canalesaguirrecristianb developmentandcharacterizationofthirtytwomicrosatellitemarkersfortheanchovyengraulisringensjenyns1842clupeiformesengraulidaevia454pyrosequencing AT herrerayanezvictoria developmentandcharacterizationofthirtytwomicrosatellitemarkersfortheanchovyengraulisringensjenyns1842clupeiformesengraulidaevia454pyrosequencing
_version_	1714205200402612224

Development and characterization of thirty-two microsatellite markers for the anchovy, Engraulis ringens Jenyns, 1842 (Clupeiformes, Engraulidae) via 454 pyrosequencing

Ejemplares similares