Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern Pacific

ABSTRACT The expansion of the shrimp industry depends on scientific advances related to the understanding of reproductive endocrinology that allows the development of aquaculture programs based on biotechnology. An insulin like-androgenic gland hormone (IAG) is synthesized in the male-specific andro...

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Autores principales:	Sancho-Blanco,Carolina, Umaña-Castro,Rodolfo, Alfaro-Montoya,Jorge
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2018
Materias:	Penaeus Penaeids IAG hormone aquaculture biotechnology RT-PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200461
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spelling	oai:scielo:S0718-560X20180002004612018-06-13Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern PacificSancho-Blanco,CarolinaUmaña-Castro,RodolfoAlfaro-Montoya,Jorge Penaeus Penaeids IAG hormone aquaculture biotechnology RT-PCR ABSTRACT The expansion of the shrimp industry depends on scientific advances related to the understanding of reproductive endocrinology that allows the development of aquaculture programs based on biotechnology. An insulin like-androgenic gland hormone (IAG) is synthesized in the male-specific androgenic gland (AG) and it is responsible for sex differentiation in crustaceans. In this study, RNA was extracted from different tissues; amplified cDNA was obtained by reverse transcription-PCR. Nucleotide sequences were analyzed following a bioinformatic approach of the encoding insulin-like androgenic gland hormone gene. This contribution presents the isolation of the partial cDNA encoding IAG from Penaeus vannamei (Lv-IAG), Penaeus occidentalis (Lo-IAG) and Penaeus stylirostris (Ls-IAG) from the distal region of terminal ampoules. In addition, the molecular characterization of these sequences is provided and the resulting aminoacid sequences were used to establish the taxonomic positioning of the IAG gene among other Decapoda species. The isolation of these partial sequences provides evidence about the anatomical location of the AG in Penaeus and is the basis for further characterize the complete cDNA of the AG-specific insulin-like encoding transcript for all species of the genus Penaeus from the Pacific coast of America.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.46 n.2 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200461en10.3856/vol46-issue2-fulltext-21
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Penaeus Penaeids IAG hormone aquaculture biotechnology RT-PCR
spellingShingle	Penaeus Penaeids IAG hormone aquaculture biotechnology RT-PCR Sancho-Blanco,Carolina Umaña-Castro,Rodolfo Alfaro-Montoya,Jorge Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern Pacific
description	ABSTRACT The expansion of the shrimp industry depends on scientific advances related to the understanding of reproductive endocrinology that allows the development of aquaculture programs based on biotechnology. An insulin like-androgenic gland hormone (IAG) is synthesized in the male-specific androgenic gland (AG) and it is responsible for sex differentiation in crustaceans. In this study, RNA was extracted from different tissues; amplified cDNA was obtained by reverse transcription-PCR. Nucleotide sequences were analyzed following a bioinformatic approach of the encoding insulin-like androgenic gland hormone gene. This contribution presents the isolation of the partial cDNA encoding IAG from Penaeus vannamei (Lv-IAG), Penaeus occidentalis (Lo-IAG) and Penaeus stylirostris (Ls-IAG) from the distal region of terminal ampoules. In addition, the molecular characterization of these sequences is provided and the resulting aminoacid sequences were used to establish the taxonomic positioning of the IAG gene among other Decapoda species. The isolation of these partial sequences provides evidence about the anatomical location of the AG in Penaeus and is the basis for further characterize the complete cDNA of the AG-specific insulin-like encoding transcript for all species of the genus Penaeus from the Pacific coast of America.
author	Sancho-Blanco,Carolina Umaña-Castro,Rodolfo Alfaro-Montoya,Jorge
author_facet	Sancho-Blanco,Carolina Umaña-Castro,Rodolfo Alfaro-Montoya,Jorge
author_sort	Sancho-Blanco,Carolina
title	Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern Pacific
title_short	Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern Pacific
title_full	Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern Pacific
title_fullStr	Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern Pacific
title_full_unstemmed	Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern Pacific
title_sort	isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp penaeus species from the eastern pacific
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000200461
work_keys_str_mv	AT sanchoblancocarolina isolationandcharacterizationofpartialinsulinlikeandrogenicglandhormonegenefromdistalterminalampoulesofthreemarineshrimppenaeusspeciesfromtheeasternpacific AT umanacastrorodolfo isolationandcharacterizationofpartialinsulinlikeandrogenicglandhormonegenefromdistalterminalampoulesofthreemarineshrimppenaeusspeciesfromtheeasternpacific AT alfaromontoyajorge isolationandcharacterizationofpartialinsulinlikeandrogenicglandhormonegenefromdistalterminalampoulesofthreemarineshrimppenaeusspeciesfromtheeasternpacific
_version_	1714205200744448000

Isolation and characterization of partial insulin-like androgenic gland hormone gene from distal terminal ampoules of three marine shrimp Penaeus species from the Eastern Pacific

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