Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus

ABSTRACT The present research was focused to characterize the digestive proteases in Macrobrachium carcinus adults using biochemical and electrophoretic techniques. Our results showed that the alkaline proteolytic activity from males and females did not show significant differences (P > 0.05) bet...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Manríquez-Santos,Teresa de Jesús, Álvarez-González,Carlos Alfonso, Peña,Emyr, Camarillo-Coop,Susana, Martínez-García,Rafael, Vega-Villasante,Fernando
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2018
Materias:	Macrobrachium carcinus enzymes nutrition proteases inhibitors
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000300525
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-560X2018000300525
record_format	dspace
spelling	oai:scielo:S0718-560X20180003005252018-08-22Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinusManríquez-Santos,Teresa de JesúsÁlvarez-González,Carlos AlfonsoPeña,EmyrCamarillo-Coop,SusanaMartínez-García,RafaelVega-Villasante,Fernando Macrobrachium carcinus enzymes nutrition proteases inhibitors ABSTRACT The present research was focused to characterize the digestive proteases in Macrobrachium carcinus adults using biochemical and electrophoretic techniques. Our results showed that the alkaline proteolytic activity from males and females did not show significant differences (P > 0.05) between them, the optimum pH for digestive proteases is 8, and is very stable to changes in alkaline pH (8 and 10). The optimum temperature for alkaline proteases is 45°C and is stable from 25 to 45°C. The activity was totally inhibited with phenylmethylsulfonyl fluoride (PMSF), additionally the inhibition with trypsin soybean inhibitor 1 (SBT1) and tosyl-lysine-methyl ketone (TLCK) indicate high effect over serine proteases. Eight active bands were found using sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) zymogram (range 17.8-94.0 kDa), which were partially inhibited with ovalbumin (Ovo), SBT1, phenanthroline (Phen), tosyl-phenylalanine-methyl ketone (TPCK), TLCK and ethylenediaminetetraacetic acid (EDTA), indicating an omnivorous digestive capacity, which remarks that the mainly alkaline protease in M. carcinus hepatopancreas are trypsin like enzymes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.46 n.3 20182018-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000300525en10.3856/vol46-issue3-fulltext-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Macrobrachium carcinus enzymes nutrition proteases inhibitors
spellingShingle	Macrobrachium carcinus enzymes nutrition proteases inhibitors Manríquez-Santos,Teresa de Jesús Álvarez-González,Carlos Alfonso Peña,Emyr Camarillo-Coop,Susana Martínez-García,Rafael Vega-Villasante,Fernando Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus
description	ABSTRACT The present research was focused to characterize the digestive proteases in Macrobrachium carcinus adults using biochemical and electrophoretic techniques. Our results showed that the alkaline proteolytic activity from males and females did not show significant differences (P > 0.05) between them, the optimum pH for digestive proteases is 8, and is very stable to changes in alkaline pH (8 and 10). The optimum temperature for alkaline proteases is 45°C and is stable from 25 to 45°C. The activity was totally inhibited with phenylmethylsulfonyl fluoride (PMSF), additionally the inhibition with trypsin soybean inhibitor 1 (SBT1) and tosyl-lysine-methyl ketone (TLCK) indicate high effect over serine proteases. Eight active bands were found using sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) zymogram (range 17.8-94.0 kDa), which were partially inhibited with ovalbumin (Ovo), SBT1, phenanthroline (Phen), tosyl-phenylalanine-methyl ketone (TPCK), TLCK and ethylenediaminetetraacetic acid (EDTA), indicating an omnivorous digestive capacity, which remarks that the mainly alkaline protease in M. carcinus hepatopancreas are trypsin like enzymes.
author	Manríquez-Santos,Teresa de Jesús Álvarez-González,Carlos Alfonso Peña,Emyr Camarillo-Coop,Susana Martínez-García,Rafael Vega-Villasante,Fernando
author_facet	Manríquez-Santos,Teresa de Jesús Álvarez-González,Carlos Alfonso Peña,Emyr Camarillo-Coop,Susana Martínez-García,Rafael Vega-Villasante,Fernando
author_sort	Manríquez-Santos,Teresa de Jesús
title	Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus
title_short	Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus
title_full	Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus
title_fullStr	Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus
title_full_unstemmed	Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus
title_sort	partial characterization of digestive proteases in adults of bigclaw river shrimp macrobrachium carcinus
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000300525
work_keys_str_mv	AT manriquezsantosteresadejesus partialcharacterizationofdigestiveproteasesinadultsofbigclawrivershrimpmacrobrachiumcarcinus AT alvarezgonzalezcarlosalfonso partialcharacterizationofdigestiveproteasesinadultsofbigclawrivershrimpmacrobrachiumcarcinus AT penaemyr partialcharacterizationofdigestiveproteasesinadultsofbigclawrivershrimpmacrobrachiumcarcinus AT camarillocoopsusana partialcharacterizationofdigestiveproteasesinadultsofbigclawrivershrimpmacrobrachiumcarcinus AT martinezgarciarafael partialcharacterizationofdigestiveproteasesinadultsofbigclawrivershrimpmacrobrachiumcarcinus AT vegavillasantefernando partialcharacterizationofdigestiveproteasesinadultsofbigclawrivershrimpmacrobrachiumcarcinus
_version_	1714205202222940160

Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus

Ejemplares similares