Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi

ABSTRACT: Mexican snook (Centropomus poeyi) is one of the most important commercial species in Southeast Mexico, which has been overexploited by fisheries. For this reason, some recent studies related to its culture have been done. However, the basic biological knowledge of the digestive physiology...

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Autores principales:	Asencio-Alcudia,Gloria Gertrudys, Tovar-Ramírez,Dariel, Latisnere-Barragán,Hever, Martínez-García,Rafael, Contreras-Sánchez,Wilfrido Miguel, Alvarez-González,Carlos Alfonso
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2018
Materias:	Centropomus poeyi snook larviculture gene expression RT-qPCR digestive enzyme
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000500909
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spelling	oai:scielo:S0718-560X20180005009092019-04-03Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyiAsencio-Alcudia,Gloria GertrudysTovar-Ramírez,DarielLatisnere-Barragán,HeverMartínez-García,RafaelContreras-Sánchez,Wilfrido MiguelAlvarez-González,Carlos Alfonso Centropomus poeyi snook larviculture gene expression RT-qPCR digestive enzyme ABSTRACT: Mexican snook (Centropomus poeyi) is one of the most important commercial species in Southeast Mexico, which has been overexploited by fisheries. For this reason, some recent studies related to its culture have been done. However, the basic biological knowledge of the digestive physiology is still unknown. In this study, the gene expression of trypsin, (TRY), bile-salt dependent lipase (BAL), amylase (AMY), and cytosolic non-specific dipeptidase (CNP) was investigated during the larval period from embryo (0 days after hatching, DAH), eleuteroembryo (1 DAH) until 25 DAH using RTq-PCR approach and was normalized with the elongation factor 1-( gene (EF1-α). TRY gene expression showed the maximum peak at 17 DAH; meanwhile, BAL gene expression showed variations during larviculture, however, three peaks were observed at 10, 17 and 25 DAH, which appears to correspond to changes in live food supply (rotifers and Artemia nauplii). AMY gene expression was low detected from hatching onwards and showed a slight peak at 10 DAH when larvae were fed with Artemia nauplii. Finally, CNP gene expression had presented three peaks at 2 DAH (yolk-sac absorption), 19 and 23 DAH when larvae were fed with Artemia nauplii. We conclude that C. poeyi larvae showed early and high digestive enzyme expressions, especially TRY, which indicate that could be possible to start the weaning process at 25 DAH, which could be shorter compared than other Centropomid species.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.46 n.5 20182018-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000500909en10.3856/vol46-issue5-fulltext-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Centropomus poeyi snook larviculture gene expression RT-qPCR digestive enzyme
spellingShingle	Centropomus poeyi snook larviculture gene expression RT-qPCR digestive enzyme Asencio-Alcudia,Gloria Gertrudys Tovar-Ramírez,Dariel Latisnere-Barragán,Hever Martínez-García,Rafael Contreras-Sánchez,Wilfrido Miguel Alvarez-González,Carlos Alfonso Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi
description	ABSTRACT: Mexican snook (Centropomus poeyi) is one of the most important commercial species in Southeast Mexico, which has been overexploited by fisheries. For this reason, some recent studies related to its culture have been done. However, the basic biological knowledge of the digestive physiology is still unknown. In this study, the gene expression of trypsin, (TRY), bile-salt dependent lipase (BAL), amylase (AMY), and cytosolic non-specific dipeptidase (CNP) was investigated during the larval period from embryo (0 days after hatching, DAH), eleuteroembryo (1 DAH) until 25 DAH using RTq-PCR approach and was normalized with the elongation factor 1-( gene (EF1-α). TRY gene expression showed the maximum peak at 17 DAH; meanwhile, BAL gene expression showed variations during larviculture, however, three peaks were observed at 10, 17 and 25 DAH, which appears to correspond to changes in live food supply (rotifers and Artemia nauplii). AMY gene expression was low detected from hatching onwards and showed a slight peak at 10 DAH when larvae were fed with Artemia nauplii. Finally, CNP gene expression had presented three peaks at 2 DAH (yolk-sac absorption), 19 and 23 DAH when larvae were fed with Artemia nauplii. We conclude that C. poeyi larvae showed early and high digestive enzyme expressions, especially TRY, which indicate that could be possible to start the weaning process at 25 DAH, which could be shorter compared than other Centropomid species.
author	Asencio-Alcudia,Gloria Gertrudys Tovar-Ramírez,Dariel Latisnere-Barragán,Hever Martínez-García,Rafael Contreras-Sánchez,Wilfrido Miguel Alvarez-González,Carlos Alfonso
author_facet	Asencio-Alcudia,Gloria Gertrudys Tovar-Ramírez,Dariel Latisnere-Barragán,Hever Martínez-García,Rafael Contreras-Sánchez,Wilfrido Miguel Alvarez-González,Carlos Alfonso
author_sort	Asencio-Alcudia,Gloria Gertrudys
title	Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi
title_short	Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi
title_full	Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi
title_fullStr	Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi
title_full_unstemmed	Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi
title_sort	gene expression of digestive enzymes during the initial ontogeny of mexican snook centropomus poeyi
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000500909
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Gene expression of digestive enzymes during the initial ontogeny of Mexican snook Centropomus poeyi

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