Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration

ABSTRACT Microbiota, the microorganisms that colonize living organisms. Oysters rely exclusively on an innate immune system. This study characterized the cultivable gastrointestinal tract microbiota (GTM) of the oyster Crassostrea gasar and evaluated their influence on immune responses. Antibiotics...

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Autores principales:	Araújo,Jaíse Paiva Bragante de, Farias,Sávio Torres de, Sousa,Oscarina Viana de, Maggioni,Rodrigo, Carvalho,Fátima Cristiane Teles de, Ramos-Queiroga,Fernando, Silva,Patricia Mirella da
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2019
Materias:	Crassostrea gasar bacterial community bivalves hemocytes phagocytosis reactive oxygen species 16S rDNA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000100078
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spelling	oai:scielo:S0718-560X20190001000782019-03-27Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administrationAraújo,Jaíse Paiva Bragante deFarias,Sávio Torres deSousa,Oscarina Viana deMaggioni,RodrigoCarvalho,Fátima Cristiane Teles deRamos-Queiroga,FernandoSilva,Patricia Mirella da Crassostrea gasar bacterial community bivalves hemocytes phagocytosis reactive oxygen species 16S rDNA ABSTRACT Microbiota, the microorganisms that colonize living organisms. Oysters rely exclusively on an innate immune system. This study characterized the cultivable gastrointestinal tract microbiota (GTM) of the oyster Crassostrea gasar and evaluated their influence on immune responses. Antibiotics were used to reduce or alter the microbiota during in vitro and in vivo assays. Haemocyte viability, concentration, and phagocytic capacity, as well as the production of reactive oxygen species (ROS), were evaluated in antibiotic-treated and control oysters. Microbiological analysis of the gastrointestinal tract was also performed; bacteria were selected using culture media and were identified by 16S ribosomal DNA. The results showed that the antibiotics eliminated bacteria in vitro but increased their concentrations in vivo. The GTM was composed of 26% amylolytic bacteria, 21% lipolytic bacteria, 18% proteolytic bacteria, 18% cellulolytic bacteria and 17% lactic acid bacteria; there were no differences in the amounts of these bacterial types between the control and treated oysters. Three major bacterial phyla, Proteobacteria, Firmicutes and Actinobacteria, and seven genera, Labrenzia, Pseudomonas, Halomonas, Shewanella, Vibrio, Bacillus, and Micrococcus, were detected. The concentration, viability, and phagocytic capacity of hemocytes and the production of ROS were not significantly altered by antibiotic treatment. In conclusion, the antibiotics altered the number of heterotrophic bacteria without changing hemocyte function, suggesting that this assay could be useful for verifying the influence of microbiota in host-parasite interactions.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.47 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000100078en10.3856/vol47-issue1-fulltext-9
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Crassostrea gasar bacterial community bivalves hemocytes phagocytosis reactive oxygen species 16S rDNA
spellingShingle	Crassostrea gasar bacterial community bivalves hemocytes phagocytosis reactive oxygen species 16S rDNA Araújo,Jaíse Paiva Bragante de Farias,Sávio Torres de Sousa,Oscarina Viana de Maggioni,Rodrigo Carvalho,Fátima Cristiane Teles de Ramos-Queiroga,Fernando Silva,Patricia Mirella da Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration
description	ABSTRACT Microbiota, the microorganisms that colonize living organisms. Oysters rely exclusively on an innate immune system. This study characterized the cultivable gastrointestinal tract microbiota (GTM) of the oyster Crassostrea gasar and evaluated their influence on immune responses. Antibiotics were used to reduce or alter the microbiota during in vitro and in vivo assays. Haemocyte viability, concentration, and phagocytic capacity, as well as the production of reactive oxygen species (ROS), were evaluated in antibiotic-treated and control oysters. Microbiological analysis of the gastrointestinal tract was also performed; bacteria were selected using culture media and were identified by 16S ribosomal DNA. The results showed that the antibiotics eliminated bacteria in vitro but increased their concentrations in vivo. The GTM was composed of 26% amylolytic bacteria, 21% lipolytic bacteria, 18% proteolytic bacteria, 18% cellulolytic bacteria and 17% lactic acid bacteria; there were no differences in the amounts of these bacterial types between the control and treated oysters. Three major bacterial phyla, Proteobacteria, Firmicutes and Actinobacteria, and seven genera, Labrenzia, Pseudomonas, Halomonas, Shewanella, Vibrio, Bacillus, and Micrococcus, were detected. The concentration, viability, and phagocytic capacity of hemocytes and the production of ROS were not significantly altered by antibiotic treatment. In conclusion, the antibiotics altered the number of heterotrophic bacteria without changing hemocyte function, suggesting that this assay could be useful for verifying the influence of microbiota in host-parasite interactions.
author	Araújo,Jaíse Paiva Bragante de Farias,Sávio Torres de Sousa,Oscarina Viana de Maggioni,Rodrigo Carvalho,Fátima Cristiane Teles de Ramos-Queiroga,Fernando Silva,Patricia Mirella da
author_facet	Araújo,Jaíse Paiva Bragante de Farias,Sávio Torres de Sousa,Oscarina Viana de Maggioni,Rodrigo Carvalho,Fátima Cristiane Teles de Ramos-Queiroga,Fernando Silva,Patricia Mirella da
author_sort	Araújo,Jaíse Paiva Bragante de
title	Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration
title_short	Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration
title_full	Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration
title_fullStr	Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration
title_full_unstemmed	Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration
title_sort	oyster (crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000100078
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Oyster (Crassostrea gasar) gastrointestinal tract microbiota and immunological responses after antibiotic administration

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